LOCUS       AA939849                 532 bp    mRNA    linear   EST 01-MAY-1998
DEFINITION  vz95e10.r1 Soares_thymus_2NbMT Mus musculus cDNA clone
            IMAGE:1345002 5' similar to gb:U05252 Mus musculus nuclear matrix
            attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AA939849
VERSION     AA939849.1
DBLINK      BioSample: SAMN00155149
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:693794
            Seq primer: -28m13 rev2 ET from Amersham
            High quality sequence stop: 443.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:1345002"
                     /sex="male"
                     /tissue_type="Thymus"
                     /clone_lib="SAMN00155149 Soares_thymus_2NbMT"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through two
                     rounds of normalization, and was constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT          120 a          120 c          146 g          146 t
ORIGIN      
        1 ctgatcggtg ttgacacaag cccagggtgc aggttcggaa gaggggtccg gacagagggc
       61 tgactgccgt gagagagctg ccaggggatg gaggctgctc agctgtgttt cctgggacag
      121 gctgctggcc aaaattgacg tggttggcac cttgctggga tagttcagac agactatcca
      181 tctcaaccat catatccttt gtcttcttga aatgtttgta ccaccttcca aattcttgac
      241 attttgctgc tgagacattt gcatagtacg tgctgttcac aatggaggag atcatgctct
      301 gtgaaagggg gcactccttg gccaacgaac tctggttcat atctttcagt aagtccttca
      361 gagcattcct tactgtggtg tgcgaccatt gttcaggagg caagtcttct agtttagggc
      421 aactgtgtaa ctgaattttg agggtgacca catgatacac atcttgaagc atgtctgcca
      481 ccgtggcatc aggggcatct gtcacatacg acagtggaac tgggattcac tt
//