LOCUS AA939849 532 bp mRNA linear EST 01-MAY-1998 DEFINITION vz95e10.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:1345002 5' similar to gb:U05252 Mus musculus nuclear matrix attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence. ACCESSION AA939849 VERSION AA939849.1 DBLINK BioSample: SAMN00155149 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 532) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:693794 Seq primer: -28m13 rev2 ET from Amersham High quality sequence stop: 443. FEATURES Location/Qualifiers source 1..532 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:1345002" /sex="male" /tissue_type="Thymus" /clone_lib="SAMN00155149 Soares_thymus_2NbMT" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 120 a 120 c 146 g 146 t ORIGIN 1 ctgatcggtg ttgacacaag cccagggtgc aggttcggaa gaggggtccg gacagagggc 61 tgactgccgt gagagagctg ccaggggatg gaggctgctc agctgtgttt cctgggacag 121 gctgctggcc aaaattgacg tggttggcac cttgctggga tagttcagac agactatcca 181 tctcaaccat catatccttt gtcttcttga aatgtttgta ccaccttcca aattcttgac 241 attttgctgc tgagacattt gcatagtacg tgctgttcac aatggaggag atcatgctct 301 gtgaaagggg gcactccttg gccaacgaac tctggttcat atctttcagt aagtccttca 361 gagcattcct tactgtggtg tgcgaccatt gttcaggagg caagtcttct agtttagggc 421 aactgtgtaa ctgaattttg agggtgacca catgatacac atcttgaagc atgtctgcca 481 ccgtggcatc aggggcatct gtcacatacg acagtggaac tgggattcac tt //