LOCUS AA789431 361 bp mRNA linear EST 06-FEB-1998 DEFINITION vw10c09.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:1243408 5' similar to gb:M97287 DNA-BINDING PROTEIN SATB1 (HUMAN); gb:U05252 Mus musculus nuclear matrix attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence. ACCESSION AA789431 VERSION AA789431.1 DBLINK BioSample: SAMN00155149 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 361) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:657096 Trace considered overall poor quality Seq primer: -28m13 rev2 ET from Amersham Sequence considered overall poor quality. FEATURES Location/Qualifiers source 1..361 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:1243408" /sex="male" /tissue_type="Thymus" /clone_lib="SAMN00155149 Soares_thymus_2NbMT" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 87 a 82 c 103 g 89 t ORIGIN 1 tttcataatg ttccacaacg cagaaaactg gtaaaatggt tcctttccaa aggttggttt 61 tcatgaggat ggcccagtgt ttttaaagga gttccctgaa attttcacct gtggtcccaa 121 agctttccct ttccaaaagg gtcccggttt ggtccaaggg gggaaaattt gggggttgga 181 cccttgggtt actcccaatt gttaggaatt ttgggagtgc ctttccccga ggttgcacgc 241 gtaaaaagaa ccaacacagt cacagtcttt aaagttaaaa ctgccggatc agggcgtgga 301 accgggacag ccggggcaac ggtgcgagcg gcaggcgagg tacggttccc acggacggcg 361 c //