LOCUS       AA771049                 486 bp    mRNA    linear   EST 29-JAN-1998
DEFINITION  vt15h08.r1 Barstead mouse myotubes MPLRB5 Mus musculus cDNA clone
            IMAGE:1163199 5' similar to gb:X14194 Mouse mRNA for entactin
            (MOUSE), mRNA sequence.
ACCESSION   AA771049
VERSION     AA771049.1
DBLINK      BioSample: SAMN00155514
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:629111
            Seq primer: -28m13 rev2 ET from Amersham.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C3H"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:1163199"
                     /cell_line="C2C12"
                     /clone_lib="SAMN00155514 Barstead mouse myotubes MPLRB5"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: EcoRI; Site_2: NotI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     [AATTCGGATCCTTG], digested with Not I and cloned into the
                     Not I and Eco RI sites of the modified pT7T3 vector.
                     Library constructed by Bob Barstead. The C2C12 cell line
                     (available from ATCC, catalog # CRL-1772) differentiates
                     rapidly, forming contractile myotubes and producing
                     characteristic muscle proteins."
BASE COUNT          138 a          130 c          103 g          115 t
ORIGIN      
        1 aattcggatc cttgcccacc tctgcttgcc cactccaggg agcaggacct gccgatgtcc
       61 tgacaacacc ctgggagttg actgcattga acggaaatga tgtcaagaat tccttgttcc
      121 ctctccaagt atttcatagt aacactctac ttgaagtgac ttgatccaga ctgaaaagtg
      181 tcctgcagtg gaatgaccac caggcccaga tctagcctag cctgagctct cacaacctct
      241 ccttaacctt ccctagaaca attaccttta gctcagtaat gggaaaatct ctaccctaac
      301 atgagggcag gacacacaca cacacacaca caccctttga tctcagtctt tagacaagtt
      361 tataaagttc tggataaaaa gccaagggta gtggtacatg cctgcaaccc tagcactagg
      421 gaggcagagg caggagaaat cgggagttca aggcccatta tcctgtctgt atagcagggt
      481 ttagaa
//