LOCUS       AA475595                 454 bp    mRNA    linear   EST 18-JUN-1997
DEFINITION  vh15g09.r1 Soares_mammary_gland_NbMMG Mus musculus cDNA clone
            IMAGE:875584 5' similar to gb:U05252 Mus musculus nuclear matrix
            attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AA475595
VERSION     AA475595.1
DBLINK      BioSample: SAMN00155466
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:515064
            Seq primer: -28m13 rev2 ET from Amersham.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:875584"
                     /sex="male"
                     /tissue_type="mammary gland"
                     /clone_lib="SAMN00155466 Soares_mammary_gland_NbMMG"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Organ: mammary gland; Vector: pT7T3D-PacI; Site_1:
                     Not I; Site_2: Eco RI; 1st strand cDNA was primed with a
                     Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATGGTTTTTTTTTTTTTTTTTTTTTTT
                     T 3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Minoru Ko, Wayne State Univ. Library
                     constructed and normalized by Bento Soares and M.Fatima
                     Bonaldo."
BASE COUNT          120 a           99 c           98 g          137 t
ORIGIN      
        1 ggggagcaca gacgttaatg ccgacttgaa agactgagag aactgtattc ttttcagcca
       61 caccaccggt atttcctaac aacatgagaa gtccaccttg tgttcactca gacaaacctt
      121 cattgtttat tgtttggcca atgaatcttc ggaaacttgc acaaacagga aggaagttgg
      181 aaggacagga cagccagcac tcaaggtttt actgtgtttt acaaaactgc ttggcagccc
      241 cgggtgaagc gtcaaggact gtttggtaga atttgtgttc aagggctgca cccaggtgtt
      301 gtaccctgtc agcatgatac cagaaattgg ttttcctttt gattattatt attctggagc
      361 ctcaaataag cattataact tctgtggtta tgattgcctt ttctttagta attcttctgt
      421 aacccaccac actagtcttt ggaaactggc ccct
//