LOCUS       AA273395                 283 bp    mRNA    linear   EST 28-MAR-1997
DEFINITION  vb97c12.r1 Soares mouse lymph node NbMLN Mus musculus cDNA clone
            IMAGE:764950 5' similar to gb:U05252 Mus musculus nuclear matrix
            attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AA273395
VERSION     AA273395.1
DBLINK      BioSample: SAMN00155172
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:465870
            Putative full length read
            vector to vector length is 284
            Seq primer: -28m13 rev2 ET from Amersham.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:764950"
                     /sex="male"
                     /tissue_type="lymph node"
                     /clone_lib="SAMN00155172 Soares mouse lymph node NbMLN"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Organ: lymph node; Vector: pT7T3D-PacI; Site_1: Not
                     I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I
                     - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGATACTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library constructed and
                     normalized by Bento Soares and M.Fatima Bonaldo."
BASE COUNT           67 a           59 c           62 g           95 t
ORIGIN      
        1 ggaaggacag gacagccagc actcaaggtt ttactgtgtt ttacaaaact gcttggcagc
       61 cccgggtgaa gcgtcaagga ctgtttggta gaatttgtgt tcaagggctg cacccaggtg
      121 ttgtaccctg tcagcatgat accagaaatt ggttttcctt ttgattatta ttattctgga
      181 gcctcaaata agcattataa cttctgtggt tatgattgcc ttttctttag taattcttct
      241 gtaacccgcc acactagtct ttggaaactg gccccttatt tta
//