LOCUS       AA153643                 464 bp    mRNA    linear   EST 19-FEB-1997
DEFINITION  mq58e05.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:582944
            5' similar to gb:M97287 DNA-BINDING PROTEIN SATB1 (HUMAN);
            gb:U05252 Mus musculus nuclear matrix attachment DNA-binding
            protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AA153643
VERSION     AA153643.1
DBLINK      BioSample: SAMN00155149
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:357592
            Seq primer: -28M13 rev2 from Amersham
            High quality sequence stop: 331.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:582944"
                     /sex="male"
                     /tissue_type="Thymus"
                     /clone_lib="SAMN00155149 Soares_thymus_2NbMT"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through two
                     rounds of normalization, and was constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT          113 a          120 c          102 g          129 t
ORIGIN      
        1 cttcagagca ttccttactg tggtgtgcga ccattgttca ggaggcaagt cttctagttt
       61 agggcaactg tgtaactgaa ttttgagggt gaccacatga tacacatctt gaagcatgtc
      121 tgccaccgtg gcatcagggg catctgtcac atacgacagt ggaactggat tccacttccc
      181 aacctggatg agccctttgg cttgggcagc agagctgtgt gaatagccta gagacagcaa
      241 cgccatctct atcagctggt tgaaaagcat atcctttctc accaatacaa attccgcgtg
      301 ctcctccttg caatcatact caatggcgtt ttcataatgt tccaccacgc agaaaactgg
      361 gtaacatggt tcctttccta aagttgggtt tccaatgaga tgccccgagt gttttaaaag
      421 gaattcccgg caacttttcc acttgtggcc ccaaaccttc cctt
//