LOCUS AA123879 592 bp mRNA linear EST 17-FEB-1997 DEFINITION mp96g08.r1 Soares_thymus_2NbMT Mus musculus cDNA clone IMAGE:577118 5' similar to gb:M97287 DNA-BINDING PROTEIN SATB1 (HUMAN); gb:U05252 Mus musculus nuclear matrix attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence. ACCESSION AA123879 VERSION AA123879.1 DBLINK BioSample: SAMN00155149 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 592) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:351766 Seq primer: -28M13 rev2 from Amersham High quality sequence stop: 411. FEATURES Location/Qualifiers source 1..592 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:577118" /sex="male" /tissue_type="Thymus" /clone_lib="SAMN00155149 Soares_thymus_2NbMT" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 133 a 129 c 164 g 166 t ORIGIN 1 catagaccta ctgaaagggg gagggtggtt caagtaatgt tggtttaagg actgctgggc 61 taagagtctg ttcactgcat actgctggtt cagcaactga gccatcacca gctgttggtt 121 gaccagctga ggactgatcg gtgttgacac aagcccaggg tgcaggttcg gaagatgggt 181 ccggacagag ggctgactgc cgtgagatag ctgcgcaggg gatggaggct gctcagctgt 241 gttttctggg acaggctgct ggccaaaatt tacgtggttg gcaccttgct gggatagttc 301 agacagacta tccatctcaa ccatcatatc ctttgtcttc ttgaaatgta tgtaccacct 361 tccaaattct tgacattttg ctgctgagac atttgcatag tacgtgctgt tcacaatgga 421 ggagatcatg ctctgtgaaa gggggcactc cttggccaac gaactctggt tcatatcttt 481 caggaagtcc ttcagagcca ttccctaccg gggctggata cgcattgtcc aggaggcaag 541 tcatccaggt ttggtgcaat tttttactta tttttttggt gaccacatga ta //