LOCUS VTR91302.1 503 aa PRT BCT 03-FEB-2020
DEFINITION Gemmata massiliana trna-i a37 thiotransferase enzyme :
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Pirellula staleyi (strain ATCC 27377 / DSM 6068 / ICPB
4128) GN=miaB PE=3 SV=1: UPF0004: Radical_SAM: TRAM protein.
ACCESSION LR593886-603
PROTEIN_ID VTR91302.1
SOURCE Gemmata massiliana
ORGANISM Gemmata massiliana
Bacteria; Planctomycetes; Planctomycetia; Gemmatales; Gemmataceae;
Gemmata.
REFERENCE 1
AUTHORS
CONSRTM Science for Life Laboratories
JOURNAL Submitted (14-MAY-2019) to the INSDC. DEPARTMENT OF CELL AND
MOLECULAR BIOLOGY, Uppsala University, Molecular Evolution,
Biomedicinskt centrum (BMC), Husargatan 3, 752 37 Uppsala, Sweden
FEATURES Qualifiers
source /organism="Gemmata massiliana"
/chromosome="1"
/isolate="Soil9"
/mol_type="genomic DNA"
/isolation_source="soil"
/db_xref="taxon:1210884"
protein /locus_tag="SOIL9_64120"
/note="BLAST_uniprot:hit_4 ;
ACCESSION=tr|L0DKZ9|L0DKZ9_SINAD ;
ALN/Q_length_ratio=0.922 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Singulisphaera acidiphila (strain ATCC BAA-1392 / DSM
18658 / VKM B-2454 / MOB10) GN=miaB PE=3 SV=1 ; EVALUE=0.0
; Q/S_length_ratio=1.059"
/note="BLAST_uniprot:hit_2 ;
ACCESSION=tr|F0SG12|F0SG12_PLABD ;
ALN/Q_length_ratio=0.928 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Planctomyces brasiliensis (strain ATCC 49424 / DSM 5305
/ JCM 21570 / NBRC 103401 / IFAM 1448) GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.012"
/note="BLAST_uniprot:hit_12 ;
ACCESSION=tr|A3ZWN1|A3ZWN1_9PLAN ;
ALN/Q_length_ratio=0.926 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Blastopirellula marina DSM 3645 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.059"
/note="BLAST_uniprot:hit_5 ;
ACCESSION=tr|A6CGG9|A6CGG9_9PLAN ;
ALN/Q_length_ratio=0.926 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Planctomyces maris DSM 8797 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=0.986"
/note="BLAST_uniprot:hit_7 ;
ACCESSION=tr|L7CFS1|L7CFS1_RHOBT ;
ALN/Q_length_ratio=0.928 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula baltica SWK14 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.057"
/note="BLAST_uniprot:hit_15 ;
ACCESSION=tr|K5DKK7|K5DKK7_RHOBT ;
ALN/Q_length_ratio=0.903 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula baltica SH28 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.086"
/note="BLAST_uniprot:hit_8 ;
ACCESSION=tr|F2AU92|F2AU92_RHOBT ;
ALN/Q_length_ratio=0.928 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula baltica WH47 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.050"
/note="BLAST_uniprot:hit_14 ;
ACCESSION=tr|M2A9S4|M2A9S4_9PLAN ;
ALN/Q_length_ratio=0.903 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula europaea 6C GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.098"
/note="BLAST_uniprot:hit_10 ;
ACCESSION=tr|M5T979|M5T979_9PLAN ;
ALN/Q_length_ratio=0.928 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula sp. SWK7 GN=miaB PE=3 SV=1 ; EVALUE=0.0
; Q/S_length_ratio=1.072"
/note="BLAST_uniprot:hit_9 ; ACCESSION=Q7ULM9 ;
ALN/Q_length_ratio=0.928 ;
DESCRIPTION=tRNA-2-methylthio-N(6)-dimethylallyladenosine
synthase OS=Rhodopirellula baltica (strain SH1) GN=miaB
PE=3 SV=1 ; EVALUE=0.0 ; Q/S_length_ratio=1.050"
/note="BLAST_uniprot:hit_3 ;
ACCESSION=tr|M5S8A6|M5S8A6_9PLAN ;
ALN/Q_length_ratio=0.924 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula maiorica SM1 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.068"
/note="BLAST_uniprot:hit_13 ;
ACCESSION=tr|M5UEP0|M5UEP0_9PLAN ;
ALN/Q_length_ratio=0.928 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula sallentina SM41 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.072"
/note="BLAST_uniprot:hit_11 ;
ACCESSION=tr|M5S4P0|M5S4P0_9PLAN ;
ALN/Q_length_ratio=0.928 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Rhodopirellula europaea SH398 GN=miaB PE=3 SV=1 ;
EVALUE=0.0 ; Q/S_length_ratio=1.068"
/note="BLAST_uniprot:hit_6 ;
ACCESSION=tr|D5SWY7|D5SWY7_PLAL2 ;
ALN/Q_length_ratio=0.917 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Planctomyces limnophilus (strain ATCC 43296 / DSM 3776
/ IFAM 1008 / 290) GN=miaB PE=3 SV=1 ; EVALUE=0.0 ;
Q/S_length_ratio=1.004"
/note="BLAST_uniprot:hit_1 ;
ACCESSION=tr|D2R676|D2R676_PIRSD ;
ALN/Q_length_ratio=0.920 ; DESCRIPTION=
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
OS=Pirellula staleyi (strain ATCC 27377 / DSM 6068 / ICPB
4128) GN=miaB PE=3 SV=1 ; EVALUE=0.0 ;
Q/S_length_ratio=1.059"
/note="Pfam_scan:hit_1 (4..103); Pfam:PF00919.15:UPF0004;
Pfam_type:Family;HMM_aln_Length:97; HMM_Length:98;
EVALUE:2.7e-30; BITSCORE: 104.0"
/note="Pfam_scan:hit_2 (163..336);
Pfam:PF04055.16:Radical_SAM;
Pfam_type:Domain;HMM_aln_Length:163; HMM_Length:166;
EVALUE:1.5e-28; BITSCORE: 100.0"
/note="Pfam_scan:hit_3 (390..464); Pfam:PF01938.15:TRAM;
Pfam_type:Domain;HMM_aln_Length:60; HMM_Length:61;
EVALUE:9.8e-08; BITSCORE: 31.6"
/note="GO_domain:GO:0005737"
/note="GO_domain:GO:0006399"
/note="GO_domain:GO:0003674"
BEGIN
1 MPQKVYIETV GCQMNVLDSE LVIGALRKRG YDLTDSPADA DVLLFNTCSV REHAEDKTYS
61 ALGRVRPIKR DNPDAVIGVL GCMAQKDQDA IRKRAPFVDM IVGTGQLGAV PDLVDKVRAT
121 RAPQLAVSLG RTDGGRNEVE ASFVSYDPAR DPSMRPTPFQ AFVRIQIGCD KFCTYCVVPS
181 TRGPEQSRPP EHIWNEVRQL VDQGCKEVTL LGQTVNSYVY ESGDTRTRLS DLIAGMHDVA
241 GLERIKFVTN YPKDMTDDLL DVVRELPKVV KYLHVPVQSG CDEVLKRMKR NYTAAYYMEM
301 LARCRELVPG VSVSSDFIVG FCGETEESFQ KSMELVRAAQ FKNSFIFKYS ERAGTKAADR
361 YPDDVPEDVK KRRNNDLLAV QNENSRIDHR AQVGKVVEVL VEGPSRREQR ASRDREAPGH
421 VTQLTGRSMA DHIVVFDGTE RLVGHTVRIA VTDASPFTLY GNVLTDEVVG VDATDREPRR
481 TPSTSSLGRD TGSRPERFSL PLV
//