LOCUS APA96610.1 158 aa PRT BCT 30-JAN-2018
DEFINITION Nocardia seriolae SsrA-binding protein protein.
ACCESSION CP017839-2519
PROTEIN_ID APA96610.1
SOURCE Nocardia seriolae
ORGANISM Nocardia seriolae
Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
Nocardiaceae; Nocardia.
REFERENCE 1 (bases 1 to 8304518)
AUTHORS Han,H.-J.
TITLE Genome sequence of Nocardia seriolae strain EM150506, isolated from
Anguila japonica
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8304518)
AUTHORS Han,H.-J.
TITLE Direct Submission
JOURNAL Submitted (21-OCT-2016) Pathology Research Division, National
Institute of Fisheries Science, #216, Gijanghaean-ro, Gijang-eup,
Gijang-gun, Busan 46083, South Korea
COMMENT DNA/bacteria available from National Institute of Fisheries Science
(NIFS) in South Korea.
##Genome-Assembly-Data-START##
Assembly Method :: SMRT analysis v. 2.3.0
Genome Coverage :: 156x
Sequencing Technology :: PacBio
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Nocardia seriolae"
/mol_type="genomic DNA"
/strain="EM150506"
/host="Anguilla japonica"
/db_xref="taxon:37332"
/geo_loc_name="South Korea"
/collection_date="2015-05-06"
protein /locus_tag="NS506_02547"
/function="Cytoplasm; RNA-binding."
/note="Required for rescue of stalled ribosomes mediated
by trans-translation. Binds to transfer-messenger RNA
(tmRNA), required for stable association of tmRNA with
ribosomes. tmRNA and SmpB together mimic tRNA shape,
replacing the anticodon stem-loop with SmpB. tmRNA is
encoded by the ssrA gen; the 2 termini fold to resemble
tRNA(Ala) and it encodes a 'tag peptide', a short internal
open reading frame. During trans-translation Ala-
aminoacylated tmRNA acts like a tRNA, entering the A-site
of stalled ribosomes, displacing the stalled mRNA. The
ribosome then switches to translate the ORF on the tmRN;
the nascent peptide is terminated with the 'tag peptide'
encoded by the tmRNA and targeted for degradation. The
ribosome is freed to recommence translation, which seems
to be the essential function of trans- translation;
Belongs to the SmpB family"
/transl_table=11
/db_xref="UniProtKB/Swiss-Prot:Q5YR60"
BEGIN
1 MKEKGRKVIA SNKKAKHNYS IIETYEAGVA LVGTEVKSLR EGKASLVDAY ASIDDGEVWL
61 RGLHVPEYGH GTWTNHAPRR TRKLLLHRRE IDKLLGKTKE SSLTLVPLSM YFSDGKVKVE
121 LALAKGKQDY DKRQDLARRD TEREITRELG RRIKGMRG
//