LOCUS AMU95041.1 1004 aa PRT BCT 21-JUN-2016
DEFINITION Sphingopyxis terrae subsp. terrae NBRC 15098 isoleucine-
-tRNA ligase protein.
ACCESSION CP013342-2069
PROTEIN_ID AMU95041.1
SOURCE Sphingopyxis terrae subsp. terrae NBRC 15098
ORGANISM Sphingopyxis terrae subsp. terrae NBRC 15098
Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
Sphingomonadaceae; Sphingopyxis.
REFERENCE 1 (bases 1 to 3979087)
AUTHORS Ohtsubo,Y., Nonoyama,S., Nagata,Y., Numata,M., Tsuchikane,K.,
Hosoyama,A., Yamazoe,A., Tsuda,M., Fujita,N. and Kawai,F.
TITLE Complete Genome Sequence of Sphingopyxis terrae Strain 203-1 (NBRC
111660), a Polyethylene Glycol Degrader
JOURNAL Genome Announc 4 (3) (2016)
PUBMED 27284143
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3979087)
AUTHORS Yoshiyuki,O.
TITLE Direct Submission
JOURNAL Submitted (30-NOV-2015) Department of Life Sciences, Tohoku
University, Katahira 2-1-1, Sendai 980-8577, Japan
COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (released 2013). Information about the Pipeline can be
found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
Annotation was conducted using PGAP and MiGAP, and PGAP annotation
was curated by referencing to the MiGAP annotation with the aid of
annotation support tool of GenomeMatcher software.
##Genome-Assembly-Data-START##
Assembly Method :: newbler v. 2.8
Genome Coverage :: 110x
Sequencing Technology :: Illumina MiSeq
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 11/30/2015 14:33:14
Annotation Pipeline :: NCBI Prokaryotic Genome Annotation
Pipeline
Annotation Method :: Best-placed reference protein set;
GeneMarkS+
Annotation Software revision :: 3.0
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes :: 3,797
CDS :: 3,699
Pseudo Genes :: 49
rRNAs :: 1, 1, 1 (5S, 16S, 23S)
complete rRNAs :: 1, 1, 1 (5S, 16S, 23S)
tRNAs :: 45
ncRNAs :: 1
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Sphingopyxis terrae subsp. terrae NBRC 15098"
/mol_type="genomic DNA"
/strain="203-1"
/sub_species="terrae"
/culture_collection="NBRC:15098"
/type_material="type strain of Sphingomonas terrae"
/db_xref="taxon:1219058"
/geo_loc_name="Japan: Kyoto"
/collection_date="1974"
protein /gene="ileS"
/locus_tag="AOA14_10530"
/EC_number="6.1.1.5"
/inference="EXISTENCE: similar to AA
sequence:RefSeq:WP_003040964.1"
/note="IleRS; catalyzes the formation of
isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since
isoleucine and other amino acids such as valine are
similar, there are additional editing function in this
enzyme; one is involved in hydrolysis of activated
valine-AMP and the other is involved in deacylation of
mischarged Val-tRNA(Ile); there are two active sites, one
for aminoacylation and one for editing; class-I
aminoacyl-tRNA synthetase family; some organisms carry two
different copies of this enzyme; Derived by automated
computational analysis using gene prediction method:
Protein Homology."
/transl_table=11
BEGIN
1 MTDSTSAPDQ RDYRDTVFLP KTDFPMKAGL PQKEPLILAK WLESNLEAQI REARRGRDQF
61 ILHDGPPYAN GDMHIGHALN HILKDMVVRT QTLKGKDAPY VPGWDCHGLP IEWKVEEQYR
121 KKKLNKDEVP VEEFRAECRA YAQHWVDTQR EQLKRLGIGG DWDHPYLTMD YEAEATIVRE
181 LLKFAANDML YRGAKPVMWS PVEKTALAEA EVEYEDIVST QIDVAFEIVE SPIAELVGAH
241 AVIWTTTPWT IPVNQALAYG PEVEYVLVAT ARGQAKMLNP GDPMPLVEDL KYLVAAPLLD
301 EFHKRIKHSV HGFAPGTVVK TIKGSDLAGT IARHPMHALG GFFARPRPFL AGDFVTTDSG
361 TGLVHMSPDH GEDDFDLCKA NGLDPVFAVE GDGKYREDWG WLGGQGSVIN PKFNAPDGPI
421 CSDLREAGAL LAASADYKHS YPHSWRSKAK IIFRCTPQWF VPMDKVMTHI EPKTRSERRW
481 EGEGGAINPA EEGLCDAPTL RQAAMQAIDD TRFVPAKGRN RIGSMVEGRP DWVLSRQRAW
541 GVPITLFVDR KTGQYLNDPL VNDRIVAAVK EAGVDAWSDA RAQEYLGDSY NAADYERITD
601 ILDVWFDSGC THAFVLESGR WPALIRHDGG THSADLYLEG SDQHRGWFQS SLLESCGTRG
661 QAPYKAVLTH GFTMDAKGFK MSKSLGNTIS PIDLMRDYGA DILRLWALSV DFTEDHRIGK
721 EILAGVADQY RKLRNTFRYL LGALDGFTEE ERITDVAAMP ELERYMLSLL ADLDAKLRQA
781 VDDFDYNAYT RLLADFCNED LSAFYFDIRK DVLYCDLGPA APLGTDTRRA YRSVLDVLFH
841 ALVRYAAPVL VFTSEEVWGT RYPDAGSVHL LEWPDFAALL RHPCEGGGPA SNDGEKLDSR
901 LRGNDELIEK WAQIRGTRAL VTERIEPLRR EKTIRSSLEA EVWLPSEAGD VDYEEIFITS
961 TVHQGDWDVK RTENHKCGRC WRHLPEVTAD GALCNRCETV LDAQ
//