LOCUS AMU95041.1 1004 aa PRT BCT 21-JUN-2016 DEFINITION Sphingopyxis terrae subsp. terrae NBRC 15098 isoleucine- -tRNA ligase protein. ACCESSION CP013342-2069 PROTEIN_ID AMU95041.1 SOURCE Sphingopyxis terrae subsp. terrae NBRC 15098 ORGANISM Sphingopyxis terrae subsp. terrae NBRC 15098 Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis. REFERENCE 1 (bases 1 to 3979087) AUTHORS Ohtsubo,Y., Nonoyama,S., Nagata,Y., Numata,M., Tsuchikane,K., Hosoyama,A., Yamazoe,A., Tsuda,M., Fujita,N. and Kawai,F. TITLE Complete Genome Sequence of Sphingopyxis terrae Strain 203-1 (NBRC 111660), a Polyethylene Glycol Degrader JOURNAL Genome Announc 4 (3) (2016) PUBMED 27284143 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3979087) AUTHORS Yoshiyuki,O. TITLE Direct Submission JOURNAL Submitted (30-NOV-2015) Department of Life Sciences, Tohoku University, Katahira 2-1-1, Sendai 980-8577, Japan COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation was conducted using PGAP and MiGAP, and PGAP annotation was curated by referencing to the MiGAP annotation with the aid of annotation support tool of GenomeMatcher software. ##Genome-Assembly-Data-START## Assembly Method :: newbler v. 2.8 Genome Coverage :: 110x Sequencing Technology :: Illumina MiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 11/30/2015 14:33:14 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 3.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 3,797 CDS :: 3,699 Pseudo Genes :: 49 rRNAs :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs :: 1, 1, 1 (5S, 16S, 23S) tRNAs :: 45 ncRNAs :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Sphingopyxis terrae subsp. terrae NBRC 15098" /mol_type="genomic DNA" /strain="203-1" /sub_species="terrae" /culture_collection="NBRC:15098" /type_material="type strain of Sphingomonas terrae" /db_xref="taxon:1219058" /geo_loc_name="Japan: Kyoto" /collection_date="1974" protein /gene="ileS" /locus_tag="AOA14_10530" /EC_number="6.1.1.5" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_003040964.1" /note="IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family; some organisms carry two different copies of this enzyme; Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MTDSTSAPDQ RDYRDTVFLP KTDFPMKAGL PQKEPLILAK WLESNLEAQI REARRGRDQF 61 ILHDGPPYAN GDMHIGHALN HILKDMVVRT QTLKGKDAPY VPGWDCHGLP IEWKVEEQYR 121 KKKLNKDEVP VEEFRAECRA YAQHWVDTQR EQLKRLGIGG DWDHPYLTMD YEAEATIVRE 181 LLKFAANDML YRGAKPVMWS PVEKTALAEA EVEYEDIVST QIDVAFEIVE SPIAELVGAH 241 AVIWTTTPWT IPVNQALAYG PEVEYVLVAT ARGQAKMLNP GDPMPLVEDL KYLVAAPLLD 301 EFHKRIKHSV HGFAPGTVVK TIKGSDLAGT IARHPMHALG GFFARPRPFL AGDFVTTDSG 361 TGLVHMSPDH GEDDFDLCKA NGLDPVFAVE GDGKYREDWG WLGGQGSVIN PKFNAPDGPI 421 CSDLREAGAL LAASADYKHS YPHSWRSKAK IIFRCTPQWF VPMDKVMTHI EPKTRSERRW 481 EGEGGAINPA EEGLCDAPTL RQAAMQAIDD TRFVPAKGRN RIGSMVEGRP DWVLSRQRAW 541 GVPITLFVDR KTGQYLNDPL VNDRIVAAVK EAGVDAWSDA RAQEYLGDSY NAADYERITD 601 ILDVWFDSGC THAFVLESGR WPALIRHDGG THSADLYLEG SDQHRGWFQS SLLESCGTRG 661 QAPYKAVLTH GFTMDAKGFK MSKSLGNTIS PIDLMRDYGA DILRLWALSV DFTEDHRIGK 721 EILAGVADQY RKLRNTFRYL LGALDGFTEE ERITDVAAMP ELERYMLSLL ADLDAKLRQA 781 VDDFDYNAYT RLLADFCNED LSAFYFDIRK DVLYCDLGPA APLGTDTRRA YRSVLDVLFH 841 ALVRYAAPVL VFTSEEVWGT RYPDAGSVHL LEWPDFAALL RHPCEGGGPA SNDGEKLDSR 901 LRGNDELIEK WAQIRGTRAL VTERIEPLRR EKTIRSSLEA EVWLPSEAGD VDYEEIFITS 961 TVHQGDWDVK RTENHKCGRC WRHLPEVTAD GALCNRCETV LDAQ //