LOCUS AEG55303.1 608 aa PRT BCT 08-JUN-2015 DEFINITION Sinorhizobium meliloti AK83 GTP-binding protein lepA protein. ACCESSION CP002781-3528 PROTEIN_ID AEG55303.1 SOURCE Sinorhizobium meliloti AK83 (Ensifer meliloti AK83) ORGANISM Sinorhizobium meliloti AK83 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. REFERENCE 1 (bases 1 to 3820344) AUTHORS Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A. TITLE Replicon-dependent bacterial genome evolution: the case of Sinorhizobium meliloti JOURNAL Genome Biol Evol 5 (3), 542-558 (2013) PUBMED 23431003 REFERENCE 2 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of chromosome1 of Sinorhizobium meliloti AK83 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4086303 Source DNA and organism available from Emanuele G. Biondi (emanuele.biondi@unifi.it) Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Sinorhizobium meliloti AK83 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Plant root, Root nodule, Soil num_replicons :: 5 ref_biomaterial :: Missing biotic_relationship :: Symbiotic trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi05780 Current Finishing Status :: Finished Funding Program :: DOE-CSP 2009 Source of Isolate :: Biondi, Emanuele (Universita di Firenze) Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 25 - 30C Gram Staining :: Gram- Symbiotic Relationship :: Mutualistic Diseases :: None Phenotypes :: Nitrogen cycle, Streptomycin resistant, Symbiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Sinorhizobium meliloti AK83" /mol_type="genomic DNA" /strain="AK83" /db_xref="taxon:693982" /chromosome="1" /collected_by="Emmanuele Biondi" protein /locus_tag="Sinme_3599" /inference="protein motif:HAMAP:MF_00071" /note="TIGRFAM: GTP-binding protein LepA; Small GTP-binding protein; HAMAP: GTP-binding protein LepA; KEGG: smd:Smed_3469 GTP-binding protein LepA; PFAM: GTP-binding protein LepA, C-terminal; Protein synthesis factor, GTP-binding; Translation elongation factor EFTu/EF1A, domain 2; Translation elongation factor EFG/EF2, C-terminal" /transl_table=11 /db_xref="InterPro:IPR000640" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR004161" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR006297" /db_xref="InterPro:IPR013842" BEGIN 1 MNTPSSKTPL SHIRNFSIVA HIDHGKSTLA DRLIQSTGGL AEREMSEQVL DSMDIERERG 61 ITIKAQTVRL HYKANDGETY VLNLIDTPGH VDFAYEVSRS LSACEGSLLV VDASQGVEAQ 121 TLANVYQAID NNHELVTVLN KIDLPAAEPE RIKEQIEEVI GIDASDAVLI SAKTGLGIPD 181 VLEAIVHKLP APKSEGGDTA PLKALLVDSW YDAYLGVMVL VRVIDGTLKK GMTIRMMGTD 241 AKYQVERVGV LTPKMVAMEA LGPGEIGFIT ASIKEVADTR VGDTITEDKR PTAKALPGFK 301 PAQPVVFCGL FPVDAADFED LRSAMGKLRL NDASFSFEME SSAALGFGFR CGFLGLLHLE 361 IIQERLEREF DLDLIATAPS VVYKLFMTDG SERELHNPAD MPDVVKIAEI HEPWIRATIL 421 TPDEYLGGIL KLCQDRRGIQ IELTYVGTRA MLTYDLPLNE VVFDFYDRLK SISKGYASFD 481 YQITEHKEGN LVKMSILVNG EPVDALSMMV HRMAAEKRGR EMCEKLKELI PKHMFKIPIQ 541 AAIGGNVIAR ETISALRKDV TAKCYGGDAT RKRKLLEKQK AGKKRMRQFG KVEIPQEAFI 601 AALKMSDE //