LOCUS AEG55213.1 767 aa PRT BCT 08-JUN-2015 DEFINITION Sinorhizobium meliloti AK83 penicillin-binding protein, 1A family protein. ACCESSION CP002781-3438 PROTEIN_ID AEG55213.1 SOURCE Sinorhizobium meliloti AK83 (Ensifer meliloti AK83) ORGANISM Sinorhizobium meliloti AK83 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. REFERENCE 1 (bases 1 to 3820344) AUTHORS Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A. TITLE Replicon-dependent bacterial genome evolution: the case of Sinorhizobium meliloti JOURNAL Genome Biol Evol 5 (3), 542-558 (2013) PUBMED 23431003 REFERENCE 2 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of chromosome1 of Sinorhizobium meliloti AK83 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4086303 Source DNA and organism available from Emanuele G. Biondi (emanuele.biondi@unifi.it) Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Sinorhizobium meliloti AK83 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Plant root, Root nodule, Soil num_replicons :: 5 ref_biomaterial :: Missing biotic_relationship :: Symbiotic trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi05780 Current Finishing Status :: Finished Funding Program :: DOE-CSP 2009 Source of Isolate :: Biondi, Emanuele (Universita di Firenze) Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 25 - 30C Gram Staining :: Gram- Symbiotic Relationship :: Mutualistic Diseases :: None Phenotypes :: Nitrogen cycle, Streptomycin resistant, Symbiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Sinorhizobium meliloti AK83" /mol_type="genomic DNA" /strain="AK83" /db_xref="taxon:693982" /chromosome="1" /collected_by="Emmanuele Biondi" protein /locus_tag="Sinme_3508" /inference="protein motif:TFAM:TIGR02074" /note="KEGG: smd:Smed_3388 1A family penicillin-binding protein; TIGRFAM: Penicillin-binding protein 1A; PFAM: Glycosyl transferase, family 51; Penicillin-binding protein, transpeptidase" /transl_table=11 /db_xref="GO:0008233" /db_xref="InterPro:IPR001264" /db_xref="InterPro:IPR001460" /db_xref="InterPro:IPR011816" BEGIN 1 MGTRSGPKGI FPSGMASGKS GQRIEPSFGG NDGDGGDLRV DASDRVAGGG SKPRRAKSPE 61 RGAKREKRGR RAGGRGSGRS GSGGGFFGLV RRLAYWCVVL GIWGAIGVGG LVLYYGARMP 121 SATSWSIPER PPNVKILAAN GDIIANRGAT GGEAVALEDM SPYIPQAVIA IEDRRFYSHF 181 GVDPLGLARA MLTNVMSGRM VQGGSTLTQQ LAKNLFLSPE RTLERKVQEV LLAFWLEQKY 241 TKDQILAMYL NRVFFGSNAY GVEAASRRYF NKSARDVNLG EAALLAGLLK APSRLSPARD 301 PEAAEERAQL VLGAMREEGF ISDSEIKTAM SQAPTRAKSF WSGAEHYVAD MVMDQVPGMI 361 GEVTQDLVID TTIDLTLEKK AEEVLAERLE TEGGKLNASQ AALVSIDGTG AIRALVGGRD 421 YAESQFDRAS KAKRQPGSAF KPFVYAAALE IGRTPMSVRN DAPVRIGNWT PENYDQKYRG 481 EVTLADALAN SLNTIAAQLV MEVGPQNVVK LAHRLGIDSD MQANASIALG TSEVTLVELT 541 SSYAPFMNGG FKATPHVIRR ISTADGTVLY ENTYDNPPRV LDPAIVSEMN RMMVRVLTDG 601 TGKNARLPGW EAAGKTGTTQ SFRDALFVGY TSNLATGIWF GNDDGKFMKK VTGGGLPARA 661 WHDFMVAAHE GLSPSPLFGT TGVQPVFDEG GIAAAGEMPA DGFDAGSPDV FPERPAGMDG 721 VESMDGMAAV DQVRPAEQAG GPIPPAGVGE TTGGTRRTTL FDLLTGG //