LOCUS       AAB59735.1               396 aa    PRT              SYN 30-SEP-2008
DEFINITION  Cloning vector pBR322 tetracycline resistance protein protein.
ACCESSION   J01749-1
PROTEIN_ID  AAB59735.1
SOURCE      Cloning vector pBR322
  ORGANISM  Cloning vector pBR322
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3; 3259 to 4361)
  AUTHORS   Sutcliffe,J.G.
  TITLE     Nucleotide sequence of the ampicillin resistance gene of
            Escherichia coli plasmid pBR322
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 75 (8), 3737-3741 (1978)
   PUBMED   358200
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Sutcliffe,J.G.
  TITLE     Complete nucleotide sequence of the Escherichia coli plasmid pBR322
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 43 (Pt 1), 77-90 (1979)
   PUBMED   383387
REFERENCE   3  (bases 1500 to 2300)
  AUTHORS   Reed,R.R., Young,R.A., Steitz,J.A., Grindley,N.D. and Guyer,M.S.
  TITLE     Transposition of the Escherichia coli insertion element gamma
            generates a five-base-pair repeat
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 76 (10), 4882-4886 (1979)
   PUBMED   388421
REFERENCE   4  (bases 2207 to 2265)
  AUTHORS   Covarrubias,L., Cervantes,L., Covarrubias,A., Soberon,X.,
            Vichido,I., Blanco,A., Kupersztoch-Portnoy,Y.M. and Bolivar,F.
  TITLE     Construction and characterization of new cloning vehicles. V.
            Mobilization and coding properties of pBR322 and several deletion
            derivatives including pBR327 and pBR328
  JOURNAL   Gene 13 (1), 25-35 (1981)
   PUBMED   6263753
REFERENCE   5  (bases 2000 to 2500)
  AUTHORS   Marians,K.J., Soeller,W. and Zipursky,S.L.
  TITLE     Maximal limits of the Escherichia coli replication factor Y
            effector site sequences in pBR322 DNA
  JOURNAL   J. Biol. Chem. 257 (10), 5656-5662 (1982)
   PUBMED   6279609
REFERENCE   6  (bases 1 to 80; 4151 to 4229; 4349 to 4361)
  AUTHORS   Brosius,J., Cate,R.L. and Perlmutter,A.P.
  TITLE     Precise location of two promoters for the beta-lactamase gene of
            pBR322. S1 mapping of ribonucleic acid isolated from Escherichia
            coli or synthesized in vitro
  JOURNAL   J. Biol. Chem. 257 (15), 9205-9210 (1982)
   PUBMED   6178738
REFERENCE   7  (bases 4241 to 4343)
  AUTHORS   Van Dyke,M.W., Hertzberg,R.P. and Dervan,P.B.
  TITLE     Map of distamycin, netropsin, and actinomycin binding sites on
            heterogeneous DNA: DNA cleavage-inhibition patterns with
            methidiumpropyl-EDTA.Fe(II)
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79 (18), 5470-5474 (1982)
   PUBMED   6291045
REFERENCE   8  (bases 584 to 709)
  AUTHORS   Peden,K.W. and Nathans,D.
  TITLE     Local mutagenesis within deletion loops of DNA heteroduplexes
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79 (23), 7214-7217 (1982)
   PUBMED   6760191
REFERENCE   9  (bases 373 to 649)
  AUTHORS   Peden,K.W.
  TITLE     Revised sequence of the tetracycline-resistance gene of pBR322
  JOURNAL   Gene 22 (2-3), 277-280 (1983)
   PUBMED   6307828
REFERENCE   10  (bases 132 to 181)
  AUTHORS   Watabe,H., Iino,T., Kaneko,T., Shibata,T. and Ando,T.
  TITLE     A new class of site-specific endodeoxyribonucleases. Endo.Sce I
            isolated from a eukaryote, Saccharomyces cerevisiae
  JOURNAL   J. Biol. Chem. 258 (8), 4663-4665 (1983)
   PUBMED   6300094
REFERENCE   11  (bases 368 to 581)
  AUTHORS   Livneh,Z.
  TITLE     Directed mutagenesis method for analysis of mutagen specificity:
            application to ultraviolet-induced mutagenesis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80 (1), 237-241 (1983)
   PUBMED   6337373
REFERENCE   12  (bases 2627 to 2682; 2781 to 2828)
  AUTHORS   Mascharak,P.K., Sugiura,Y., Kuwahara,J., Suzuki,T. and Lippard,S.J.
  TITLE     Alteration and activation of sequence-specific cleavage of DNA by
            bleomycin in the presence of the antitumor drug
            cis-diamminedichloroplatinum(II)
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80 (22), 6795-6798 (1983)
   PUBMED   6196777
REFERENCE   13  (bases 4276 to 4336)
  AUTHORS   Schultz,P.G. and Dervan,P.B.
  TITLE     Sequence-specific double-strand cleavage of DNA by
            penta-N-methylpyrrolecarboxamide-EDTA X Fe(II)
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80 (22), 6834-6837 (1983)
   PUBMED   6417654
REFERENCE   14  (bases 518 to 528)
  AUTHORS   Sutcliffe,J.G.
  JOURNAL   Unpublished
REFERENCE   15  (bases 2395 to 2495)
  AUTHORS   Fuller,R.S., Funnell,B.E. and Kornberg,A.
  TITLE     The dnaA protein complex with the E. coli chromosomal replication
            origin (oriC) and other DNA sites
  JOURNAL   Cell 38 (3), 889-900 (1984)
   PUBMED   6091903
REFERENCE   16  (bases 2729 to 2731)
  AUTHORS   Lathe,R., Kieny,M.P., Skory,S. and Lecocq,J.P.
  TITLE     Linker tailing: unphosphorylated linker oligonucleotides for
            joining DNA termini
  JOURNAL   DNA 3 (2), 173-182 (1984)
   PUBMED   6327214
REFERENCE   17  (bases 2729 to 2730)
  AUTHORS   Heusterspreute,M. and Davison,J.
  TITLE     Restriction site bank vectors. II. DNA sequence analysis of plasmid
            pJRD158
  JOURNAL   DNA 3 (3), 259-268 (1984)
   PUBMED   6086259
REFERENCE   18  (bases 2113 to 2186; 2348 to 2415)
  AUTHORS   Abarzua,P., Soeller,W. and Marians,K.J.
  TITLE     Mutational analysis of primosome assembly sites. I. Distinct
            classes of mutants in the pBR322 Escherichia coli factor Y DNA
            effector sequences
  JOURNAL   J. Biol. Chem. 259 (22), 14286-14292 (1984)
   PUBMED   6209275
REFERENCE   19  (bases 2348 to 2415)
  AUTHORS   Soeller,W., Abarzua,P. and Marians,K.J.
  TITLE     Mutational analysis of primosome assembly sites. II. Role of
            secondary structure in the formation of active sites
  JOURNAL   J. Biol. Chem. 259 (22), 14293-14300 (1984)
   PUBMED   6150042
REFERENCE   20  (bases 1 to 4361)
  AUTHORS   Van Dyke,M.M. and Dervan,P.B.
  TITLE     Echinomycin binding sites on DNA
  JOURNAL   Science 225 (4667), 1122-1127 (1984)
   PUBMED   6089341
REFERENCE   21  (sites)
  AUTHORS   Pouwels,P.H., Enger-Valk,B.E. and Brammar,W.J.
  JOURNAL   (in) CLONING VECTORS. Elsevier Scientific Publishing, Amsterdam
            (1985)
  REMARK    Vector I-A-iv-1
REFERENCE   22  (bases 1 to 4361)
  AUTHORS   Watson,N.
  TITLE     A new revision of the sequence of plasmid pBR322
  JOURNAL   Gene 70 (2), 399-403 (1988)
   PUBMED   3063608
REFERENCE   23  (sites)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
COMMENT     On or before Apr 4, 2002 this sequence version replaced L08654.1,
            gi:58257.
            The circular sequence is numbered such that 0 is the middle of the
            unique EcoRI site and the count increases first through the tet
            genes, the pMB1 material, and finally through the Tn3 region.
            Plasmid pBR322 contains ampicillin and tetracycline resistance
            genes.  The ampicillin resistance gene (amp-r) is a penicillin
            beta-lactamase. Promoters P1 and P3 are for the beta-lactamase
            gene. P3 is the natural promoter, and P1 is artificially created by
            the ligation of two different DNA fragments to create pBR322. P2 is
            in the same region as P1, but it is on the opposite strand and
            initiates transcription in the direction of the tetracycline
            resistance gene.
            Mutational studies in the primosome assembly sites indicate four
            types of mutations:  Class I having no effect on the activities
            elicited by the DNA site and the bases involved are probably
            spacers; Class II requiring higher Mg-2+ concentrations than the
            wild-type to be fully activated as factor Y ATPase effectors; Class
            III co-inactivating both the ATPase effector and DNA replication
            template activity of the site, indicating that they probably
            represent essential contact points between factor Y and the DNA;
            Class IV having a replication template activity intermediate that
            of class III and class II mutant DNAs.
            Specific sites within or near the origins of replication are
            recognized by dnaA protein.  Without dnaA binding to the origin of
            replication chromosomal replication is not possible (15). pBR322
            DNA contains two separate regions on opposite strands and close to
            the origin of replication which, when in single-stranded form, can
            act as effectors for the ATPase activity of E.coli replication
            factor Y (5).  Small fragments of DNA containing these sites when
            cloned in an f1 phage vector act as origins of DNA replication
            allowing the formation of complementary double-stranded DNA in
            rifampicin-resistant, dna(B,G,C)-dependent fashion in vitro (5).
            The biological activity of echinomycin is thought to be related to
            the formation of complexes by intercalating with cellular DNA (20).
            Complete source information:
            Plasmid pBR322 from E.coli (2),(1),(3),(6),(11),(8),(5),(7),(12),
            (13),(10),(9),(14),(18),(19),(15),(20),(16); pBR322 DNA in pXf3
            (4).
            The following data and their annotation were supplied by Will
            Gilbert under the auspices of the Curator Program.
             CROSSREFERENCE
                           #parent
                             GenBank(50):pSC101C, GenBank(50):Trn3
                           #offspring
                             VecBase(3):pBR325, VecBase(3):pBR327,
            VecBase(3):pBR328,
                             VecBase(3):pAT153, VecBase(3):pUC7,
            VecBase(3):pJRD158,
                             VecBase(3):PiVX, VecBase(3):PiAN7,
            VecBase(3):pSP64,
                             VecBase(3):pSP65, VecBase(3):pGEM1,
            VecBase(3):pGEM2,
                             VecBase(3):pGEM3, VecBase(3):pGEM4,
            VecBase(3):pKK223,
                             VecBase(3):pLBU3, VecBase(3):pTrS3,
            VecBase(3):pRSVNeo,
                             VecBase(3):pSV2Cat, VecBase(3):M13mp9,
            VecBase(3):pHC79,
                             VecBase(3):pV34, VecBase(3):pKTH601,
            VecBase(3):pKTH604,
                             VecBase(3):pKTH605, VecBase(3):pKTH606,
            VecBase(3):YEp24,
                             VecBase(3):YIp5, VecBase(3):YRp17,
            VecBase(3):pSP18,
                        VecBase(3):pSP19,
                             VecBase(3):pSP6T3, VecBase(3):pSP6T719,
            VecBase(3):pT712,
                             VecBase(3):pT713, VecBase(3):pT7T318,
            VecBase(3):pT7T319,
                             VecBase(3):pT7T3A18, VecBase(3):pT7T3A19,
            VecBase(3):pEX1,
                             VecBase(3):pEX2, VecBase(3):pEX3,
            VecBase(3):pCKSP6,
                             VecBase(3):pACYC177, VecBase(3):pKO1,
            VecBase(3):pKO2,
                        VecBase(3):pKM1,
                             VecBase(3):pKM2, VecBase(3):pMBL1,
            VecBase(3):pMBL604,
                             VecBase(3):pMC1511, VecBase(3):pMC1871,
            VecBase(3):pAA37X,
                             VecBase(3):pUR278, VecBase(3):pUR288,
            VecBase(3):pUR289,
                             VecBase(3):pUR290, VecBase(3):pUR291,
            VecBase(3):pUR292,
                             VecBase(3):pUR222.
FEATURES             Qualifiers
     source          /organism="Cloning vector pBR322"
                     /mol_type="other DNA"
                     /db_xref="taxon:47470"
                     /tissue_lib="ATCC 31344, ATCC 37017"
                     /focus
     source          /organism="Plasmid pSC101"
                     /mol_type="other DNA"
                     /db_xref="taxon:2625"
     protein         /gene="tet"
                     /transl_table=11
BEGIN
        1 MKSNNALIVI LGTVTLDAVG IGLVMPVLPG LLRDIVHSDS IASHYGVLLA LYALMQFLCA
       61 PVLGALSDRF GRRPVLLASL LGATIDYAIM ATTPVLWILY AGRIVAGITG ATGAVAGAYI
      121 ADITDGEDRA RHFGLMSACF GVGMVAGPVA GGLLGAISLH APFLAAAVLN GLNLLLGCFL
      181 MQESHKGERR PMPLRAFNPV SSFRWARGMT IVAALMTVFF IMQLVGQVPA ALWVIFGEDR
      241 FRWSATMIGL SLAVFGILHA LAQAFVTGPA TKRFGEKQAI IAGMAADALG YVLLAFATRG
      301 WMAFPIMILL ASGGIGMPAL QAMLSRQVDD DHQGQLQGSL AALTSLTSIT GPLIVTAIYA
      361 ASASTWNGLA WIVGAALYLV CLPALRRGAW SRATST
//