LOCUS AEG53881.1 486 aa PRT BCT 08-JUN-2015 DEFINITION Sinorhizobium meliloti AK83 UDP-N-acetylmuramoyl-L-alanyl- D-glutamate--2, 6-diaminopimelate ligase protein. ACCESSION CP002781-2106 PROTEIN_ID AEG53881.1 SOURCE Sinorhizobium meliloti AK83 (Ensifer meliloti AK83) ORGANISM Sinorhizobium meliloti AK83 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. REFERENCE 1 (bases 1 to 3820344) AUTHORS Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A. TITLE Replicon-dependent bacterial genome evolution: the case of Sinorhizobium meliloti JOURNAL Genome Biol Evol 5 (3), 542-558 (2013) PUBMED 23431003 REFERENCE 2 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of chromosome1 of Sinorhizobium meliloti AK83 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4086303 Source DNA and organism available from Emanuele G. Biondi (emanuele.biondi@unifi.it) Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Sinorhizobium meliloti AK83 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Plant root, Root nodule, Soil num_replicons :: 5 ref_biomaterial :: Missing biotic_relationship :: Symbiotic trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi05780 Current Finishing Status :: Finished Funding Program :: DOE-CSP 2009 Source of Isolate :: Biondi, Emanuele (Universita di Firenze) Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 25 - 30C Gram Staining :: Gram- Symbiotic Relationship :: Mutualistic Diseases :: None Phenotypes :: Nitrogen cycle, Streptomycin resistant, Symbiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Sinorhizobium meliloti AK83" /mol_type="genomic DNA" /strain="AK83" /db_xref="taxon:693982" /chromosome="1" /collected_by="Emmanuele Biondi" protein /locus_tag="Sinme_2161" /EC_number="6.3.2.13" /inference="protein motif:HAMAP:MF_00208" /note="PFAM: Mur ligase, central; Mur ligase, N-terminal; Mur ligase, C-terminal; TIGRFAM: UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase; HAMAP: UDP-N-acetylmuramoylalanyl-D-glutamate-2,6- diaminopimelate ligase; KEGG: smd:Smed_2087 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase" /transl_table=11 /db_xref="InterPro:IPR000713" /db_xref="InterPro:IPR004101" /db_xref="InterPro:IPR005761" /db_xref="InterPro:IPR013221" BEGIN 1 MKITDLAGSN FPELSAQLKG DAATIEIGGI TADSRQVKPG DLFVAVAGSK ADGAAYIADA 61 LSRGASAVVA GTGTPAEAGA PVFAISDPRR FLAKAASSFY GRQPETMVAV TGTAGKTSVA 121 SFTRQIWAHS GFSAAMIGTT GVVAPGRTEY GSLTTPDPVS LHKLLAELAD EGVTHAAMEA 181 SSHGLDQRRL DGVELAAAAF TNLGRDHMDY HPTVEHYMAS KMRLFGALLP KGSPAVIFAD 241 DQWSAEAIAA ARKAGHDVRT VGRNGDFIAL KRVEHFRHKQ SAEVHVGDDI YEIHVPLAGD 301 FQIANALVAA GLAMSTGITA KAAFSALERL QGASGRLELV GQTKDGALAY VDYAHKPDAL 361 ANVLESVRPF TTGRVVVVFG CGGDRDKGKR PIMGEIASRL ADVVIVTDDN PRSEVPEVIR 421 AEIMAAAKGA TEIGDRAEAI RAAVGMLKTG DTLIVAGKGH EEGQTVGSVT LPFSDHAEVR 481 KALGGL //