LOCUS AEG52023.1 602 aa PRT BCT 08-JUN-2015 DEFINITION Sinorhizobium meliloti AK83 phage/plasmid primase, P4 family protein. ACCESSION CP002781-248 PROTEIN_ID AEG52023.1 SOURCE Sinorhizobium meliloti AK83 (Ensifer meliloti AK83) ORGANISM Sinorhizobium meliloti AK83 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. REFERENCE 1 (bases 1 to 3820344) AUTHORS Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A. TITLE Replicon-dependent bacterial genome evolution: the case of Sinorhizobium meliloti JOURNAL Genome Biol Evol 5 (3), 542-558 (2013) PUBMED 23431003 REFERENCE 2 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of chromosome1 of Sinorhizobium meliloti AK83 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4086303 Source DNA and organism available from Emanuele G. Biondi (emanuele.biondi@unifi.it) Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Sinorhizobium meliloti AK83 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Plant root, Root nodule, Soil num_replicons :: 5 ref_biomaterial :: Missing biotic_relationship :: Symbiotic trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi05780 Current Finishing Status :: Finished Funding Program :: DOE-CSP 2009 Source of Isolate :: Biondi, Emanuele (Universita di Firenze) Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 25 - 30C Gram Staining :: Gram- Symbiotic Relationship :: Mutualistic Diseases :: None Phenotypes :: Nitrogen cycle, Streptomycin resistant, Symbiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Sinorhizobium meliloti AK83" /mol_type="genomic DNA" /strain="AK83" /db_xref="taxon:693982" /chromosome="1" /collected_by="Emmanuele Biondi" protein /locus_tag="Sinme_0254" /inference="protein motif:TFAM:TIGR01613" /note="TIGRFAM: DNA primase, phage/plasmid; PFAM: Phage/plasmid primase, P4, C-terminal; KEGG: bbt:BBta_6606 putative phage / plasmid primase P4; SMART: Phage/plasmid primase, P4, C-terminal" /transl_table=11 /db_xref="InterPro:IPR006500" /db_xref="InterPro:IPR014818" BEGIN 1 MAERKKRPTL PEDVAQKLEE FSAQREPENP ETPLGPSDEP PAHLSADLEP DEAAVEYCAE 61 FDHSDTDNGK RLITHFGTDF IVLAQEKAKQ PAFAVWTGSH WDVSNGGPKA TAIAQRLGDR 121 IAREVAYIKP NAFEQMLIDK AIEARKKPEE DRNPTEKRLA IAAEKAEEAH GKRVKRRLDH 181 AVTSKNVAKM QAALSCAAPH IMRSPNDFNA NRMMVAVQNA TLVFHRKTER RKNPRHKSVS 241 ETPDAPEYID ACVDSSLEVI PGHRRRDMIT HIVPVRYRPE AKCSKWMAFI ESKLPDKDVR 301 RLVQVSSGLG LLGITVQYLF FHYGDGANGK SVYMETLCRL LGDVAVTLPA TSLIGEGGSS 361 GSASPDLARL HGRRLLRVKE LPEGEDLREN LVKELTGGET ITARDLFSGY TDFLPIFIAI 421 MSGNGYPRIT GTDDGIWRRM AVIHWPNKIA KEDRREFEEI VSSFEPEHPG ILNWLIEGAH 481 IFLREGLVIP EAVEKATQEY RDDMDRTAGF VGRCIERDAN ADPLQGKDLY QAYCDDTVDQ 541 GGKPMNVTAF GRVMSKKFRK ERTATGVVYF GVRLKNVPSG SSMRGPPAGR FDPNEPWPDD 601 LP //