LOCUS AEG51789.1 514 aa PRT BCT 08-JUN-2015 DEFINITION Sinorhizobium meliloti AK83 RNA polymerase, sigma 54 subunit, RpoN protein. ACCESSION CP002781-14 PROTEIN_ID AEG51789.1 SOURCE Sinorhizobium meliloti AK83 (Ensifer meliloti AK83) ORGANISM Sinorhizobium meliloti AK83 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. REFERENCE 1 (bases 1 to 3820344) AUTHORS Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A. TITLE Replicon-dependent bacterial genome evolution: the case of Sinorhizobium meliloti JOURNAL Genome Biol Evol 5 (3), 542-558 (2013) PUBMED 23431003 REFERENCE 2 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of chromosome1 of Sinorhizobium meliloti AK83 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4086303 Source DNA and organism available from Emanuele G. Biondi (emanuele.biondi@unifi.it) Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Sinorhizobium meliloti AK83 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Plant root, Root nodule, Soil num_replicons :: 5 ref_biomaterial :: Missing biotic_relationship :: Symbiotic trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi05780 Current Finishing Status :: Finished Funding Program :: DOE-CSP 2009 Source of Isolate :: Biondi, Emanuele (Universita di Firenze) Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 25 - 30C Gram Staining :: Gram- Symbiotic Relationship :: Mutualistic Diseases :: None Phenotypes :: Nitrogen cycle, Streptomycin resistant, Symbiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Sinorhizobium meliloti AK83" /mol_type="genomic DNA" /strain="AK83" /db_xref="taxon:693982" /chromosome="1" /collected_by="Emmanuele Biondi" protein /locus_tag="Sinme_0014" /inference="protein motif:TFAM:TIGR02395" /note="KEGG: smd:Smed_0015 RNA polymerase factor sigma-54; TIGRFAM: RNA polymerase sigma factor 54; PFAM: RNA polymerase sigma factor 54, core-binding; RNA polymerase sigma factor 54; RNA polymerase sigma factor 54, DNA-binding" /transl_table=11 /db_xref="GO:0003677" /db_xref="GO:0003700" /db_xref="GO:0003899" /db_xref="GO:0016987" /db_xref="InterPro:IPR000394" /db_xref="InterPro:IPR007046" /db_xref="InterPro:IPR007634" BEGIN 1 MALSASLHLR QSQSLVMTPQ LMQSIQLLQM NHLELSHFIA QEVEKNPLLE VQPADEPTIS 61 DREDAGPHPA ETGGETDEAA GQSDLYDSAM SRSGERLSEG LDADFANVFP DDTAPQRADA 121 PELLGQWKSM PGAGDAEGYD LDDFVGGRKT LRETLAEQLP FALSAVSDRL IARYFIDQLD 181 DAGYLHADLA ETAETLGAAG EDVARVLHVL QQFDPPGVFA RTLGECLAIQ LRARNRLDPA 241 MEALVANLEL LARRDFASLK KICGVDEEDL IDMLAEIRKL DPKPGTSFET GVFEAIIPDV 301 VVRAAPDGGW LVELNPDALP RVLVNHDYFT EISRSSRKNS GEQAFLNECL QNANWLTRSL 361 DQRARTIMKV ASEIVRQQDA FLIHGVGHLR PLNLRIVADA IKMHESTVSR VTSNKYMLTP 421 RGLFELKYFF TVSIGSAENG DAHSAESVRH RIRTMINQES ADAVLSDDDI VDVLKQAGVD 481 IARRTVAKYR EAMSIPSSVQ RRREKRALAK AAGF //