LOCUS BC070256 1244 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens interferon, gamma, mRNA (cDNA clone MGC:88243
IMAGE:30414644), complete cds.
ACCESSION BC070256
VERSION BC070256.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1244)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1244)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 57 Row: p Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56786137.
FEATURES Location/Qualifiers
source 1..1244
/db_xref="H-InvDB:HIT000264214"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88243 IMAGE:30414644"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1244
/gene="IFNG"
/gene_synonym="IFG"
/gene_synonym="IFI"
/db_xref="GeneID:3458"
/db_xref="HGNC:HGNC:5438"
/db_xref="MIM:147570"
CDS 129..629
/gene="IFNG"
/gene_synonym="IFG"
/gene_synonym="IFI"
/codon_start=1
/product="interferon, gamma"
/protein_id="AAH70256.1"
/db_xref="GeneID:3458"
/db_xref="HGNC:HGNC:5438"
/db_xref="MIM:147570"
/translation="MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSD
VADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNV
KFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFR
GRRASQ"
BASE COUNT 434 a 205 c 230 g 375 t
ORIGIN
1 agcacattgt tctgatcatc tgaagatcag ctattagaag agaaagatca gttaagtcct
61 ttggacctga tcagcttgat acaagaacta ctgatttcaa cttctttggc ttaattctct
121 cggaaacgat gaaatataca agttatatct tggcttttca gctctgcatc gttttgggtt
181 ctcttggctg ttactgccag gacccatatg taaaagaagc agaaaacctt aagaaatatt
241 ttaatgcagg tcattcagat gtagcggata atggaactct tttcttaggc attttgaaga
301 attggaaaga ggagagtgac agaaaaataa tgcagagcca aattgtctcc ttttacttca
361 aactttttaa aaactttaaa gatgaccaga gcatccaaaa gagtgtggag accatcaagg
421 aagacatgaa tgtcaagttt ttcaatagca acaaaaagaa acgagatgac ttcgaaaagc
481 tgactaatta ttcggtaact gacttgaatg tccaacgcaa agcaatacat gaactcatcc
541 aagtgatggc tgaactgtcg ccagcagcta aaacagggaa gcgaaaaagg agtcagatgc
601 tgtttcgagg tcgaagagca tcccagtaat ggttgtcctg cctgcaatat ttgaatttta
661 aatctaaatc tatttattaa tatttaacat tatttatatg gggaatatat ttttagactc
721 atcaatcaaa taagtattta taatagcaac ttttgtgtaa tgaaaatgaa tatctattaa
781 tatatgtatt atttataatt cctatatcct gtgactgtct cacttaatcc tttgttttct
841 gactaattag gcaaggctat gtgattacaa ggctttatct caggggccaa ctaggcagcc
901 aacctaagca agatcccatg ggttgtgtgt ttatttcact tgatgataca atgaacactt
961 ataagtgaag tgatactatc cagttactgc cggtttgaaa atatgcctgc aatttgagcc
1021 agtgctttaa tggcatgtca gacagaactt gaatgtgtca ggtgaccctg atgaaaacat
1081 agcatctcag gagatttcat gcctggtgct tccaaatatt gttgacaact gtgactgtac
1141 ccaaatggaa agtaactcat ttgttaaaat tatcaatatc taatatatat gaataaagtg
1201 taagttcaca actgaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//