LOCUS BC064506 2362 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens G protein-coupled receptor kinase 5, mRNA (cDNA clone
MGC:71228 IMAGE:6482467), complete cds.
ACCESSION BC064506
VERSION BC064506.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2362)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2362)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 50 Row: n Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 51896033.
FEATURES Location/Qualifiers
source 1..2362
/db_xref="H-InvDB:HIT000261425"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71228 IMAGE:6482467"
/tissue_type="Prostate, carcinoma"
/clone_lib="NIH_MGC_40"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2362
/gene="GRK5"
/db_xref="GeneID:2869"
/db_xref="HGNC:HGNC:4544"
/db_xref="MIM:600870"
CDS 228..2000
/gene="GRK5"
/codon_start=1
/product="G protein-coupled receptor kinase 5"
/protein_id="AAH64506.1"
/db_xref="GeneID:2869"
/db_xref="HGNC:HGNC:4544"
/db_xref="MIM:600870"
/translation="MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCED
LRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKG
KEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHE
YLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLE
KKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIY
NMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAV
KIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKV
KREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRN
MNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVS
IPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSS
PSSKTSFNHHINSNHVSSNSTGSS"
BASE COUNT 610 a 605 c 688 g 459 t
ORIGIN
1 gggacacaga gggaggaaga agcggcggcg gcggcggcgg cggctcctct ttgcagaggg
61 ggaaactctt gggctgagag caggaataat gcggtaggca aggcgggctg ctggctcccc
121 cggctccggc agcagcggcg gcagcccgag cagcggcagc agcagcggca gcatcccagg
181 cgctgacagc cccgccggcc ggctccgttg ctgaccgccg actgtcaatg gagctggaaa
241 acatcgtggc caacacggtc ttgctgaaag ccagggaagg gggcggagga aagcgcaaag
301 ggaaaagcaa gaagtggaaa gaaatcctga agttccctca cattagccag tgtgaagacc
361 tccgaaggac catagacaga gattactgca gtttatgtga caagcagcca atcgggaggc
421 tgcttttccg gcagttttgt gaaaccaggc ctgggctgga gtgttacatt cagttcctgg
481 actccgtggc agaatatgaa gttactccag atgaaaaact gggagagaaa gggaaggaaa
541 ttatgaccaa gtacctcacc ccaaagtccc ctgttttcat agcccaagtt ggccaagacc
601 tggtctccca gacggaggag aagctcctac agaagccgtg caaagaactc ttttctgcct
661 gtgcacagtc tgtccacgag tacctgaggg gagaaccatt ccacgaatat ctggacagca
721 tgttttttga ccgctttctc cagtggaagt ggttggaaag gcaaccggtg accaaaaaca
781 ctttcaggca gtatcgagtg ctaggaaaag ggggcttcgg ggaggtctgt gcctgccagg
841 ttcgggccac gggtaaaatg tatgcctgca agcgcttgga gaagaagagg atcaaaaaga
901 ggaaagggga gtccatggcc ctcaatgaga agcagatcct cgagaaggtc aacagtcagt
961 ttgtggtcaa cctggcctat gcctacgaga ccaaggatgc actgtgcttg gtcctgacca
1021 tcatgaatgg gggtgacctg aagttccaca tctacaacat gggcaaccct ggcttcgagg
1081 aggagcgggc cttgttttat gcggcagaga tcctctgcgg cttagaagac ctccaccgtg
1141 agaacaccgt ctaccgagat ctgaaacctg aaaacatcct gttagatgat tatggccaca
1201 ttaggatctc agacctgggc ttggctgtga agatccccga gggagacctg atccgcggcc
1261 gggtgggcac tgttggctac atggctccag aggtcctgaa caaccagagg tacggcctga
1321 gccccgacta ctggggcctt ggctgcctca tctatgagat gatcgagggc cagtcgccgt
1381 tccgcggccg caaggagaag gtgaagcggg aggaggtgga ccgccgggtc ctggagacgg
1441 aggaggtgta ctcccacaag ttctccgagg aggccaagtc catctgcaag atgctgctca
1501 cgaaagatgc gaagcagagg ctgggctgcc aggaggaggg ggctgcagag gtcaagagac
1561 accccttctt caggaacatg aacttcaagc gcttagaagc cgggatgttg gaccctccct
1621 tcgttccaga cccccgcgct gtgtactgta aggacgtgct ggacatcgag cagttctcca
1681 ctgtgaaggg cgtcaatctg gaccacacag acgacgactt ctactccaag ttctccacgg
1741 gctctgtgtc catcccatgg caaaacgaga tgatagaaac agaatgcttt aaggagctga
1801 acgtgtttgg acctaatggt accctcccgc cagatctgaa cagaaaccac cctccggaac
1861 cgcccaagaa agggctgctc cagagactct tcaagcggca gcatcagaac aattccaaga
1921 gttcgcccag ctccaagacc agttttaacc accacataaa ctcaaaccat gtcagctcga
1981 actccaccgg aagcagctag tttcggctct ggcctccaag tccacagtgg aaccagccca
2041 gacccttctc cttagaagtg gaagtagtgg agcccctgct ctggtggggc tgccagggga
2101 gaccccggga gccggggaag gaggccgtcc atcccgtcga cgtagaacct cgaggtttct
2161 caaagaaatt tccactcagg tctgttttcc gaggcggccc cggccggggt ggattggatt
2221 tgtctttggt gaacattgca atagaaatcc aattggatac gacaacttgc acgtatttta
2281 atagcgtcat aactagaact gaattttgtc tttatgattt ttaaagaaaa gttttgtaaa
2341 tttcaaaaaa aaaaaaaaaa aa
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