LOCUS BC030553 2174 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sphingosine kinase 1, mRNA (cDNA clone MGC:40267
IMAGE:5213270), complete cds.
ACCESSION BC030553
VERSION BC030553.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2174)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2174)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 64 Row: k Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41282001.
FEATURES Location/Qualifiers
source 1..2174
/db_xref="H-InvDB:HIT000050991"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:40267 IMAGE:5213270"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2174
/gene="SPHK1"
/gene_synonym="SPHK"
/db_xref="GeneID:8877"
/db_xref="HGNC:HGNC:11240"
/db_xref="MIM:603730"
CDS 457..1869
/gene="SPHK1"
/gene_synonym="SPHK"
/codon_start=1
/product="sphingosine kinase 1"
/protein_id="AAH30553.1"
/db_xref="GeneID:8877"
/db_xref="HGNC:HGNC:11240"
/db_xref="MIM:603730"
/translation="MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPTATAPGGEGEP
HSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCR
VLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDA
LVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNE
DLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEM
RFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH
WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAM
EKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG
CVEPPPSWKPQQMPPPEEPL"
BASE COUNT 376 a 683 c 729 g 386 t
ORIGIN
1 gaaaagtttg aggccggagg gagcgaggcc ggggagtccg ctccagcggg gcgctccagt
61 ccctcagacg tgggctgagc ttgggacgag ctgcgttccg ccccaggcca ctgtagggaa
121 cggcggtggc gcctccccag caaaccggac cgactgggta gggccgccca ccctgccttc
181 gcgccgctcg tggctcctgt gcggcccgcc ctcgcggggc cccgggaact gggccacttg
241 tcgcttgggc gagagcaggc ggcagctggt ggcccggttc gggcttggct ttggcgcgac
301 ccgggagcgg ctcccacgag cgccgcgcgc gtcgcaacgg agcggggccc tgagaagcgc
361 gcgccgcggc tcccaccgct ctggagctcc gggcagggga cacggcaacc tggatggctg
421 gggcagggat cctctcccag aacttcgtgc ccagcaatgt ccgctcaagt tctgggattt
481 ttacgcagct ggactcccct ccccctggca gccccgaggg gtccagccgc cgcagggaat
541 gacgccggtg ctcctacagc cacggctccg ggcggggaag gcgagcccca cagccggccc
601 tgcgacgccc gcctgggcag caccgataag gagctgaagg caggagccgc cgccacgggc
661 agcgccccca cagcgccagg gaccccctgg cagcgggagc cgcgggtcga ggttatggat
721 ccagcgggcg gcccccgggg cgtgctcccg cggccctgcc gcgtgctggt gctgctgaac
781 ccgcgcggcg gcaagggcaa ggccttgcag ctcttccgga gtcacgtgca gccccttttg
841 gctgaggctg aaatctcctt cacgctgatg ctcactgagc ggcggaacca cgcgcgggag
901 ctggtgcggt cggaggagct gggccgctgg gacgctctgg tggtcatgtc tggagacggg
961 ctgatgcacg aggtggtgaa cgggctcatg gagcggcctg actgggagac cgccatccag
1021 aagcccctgt gtagcctccc agcaggctct ggcaacgcgc tggcagcttc cttgaaccat
1081 tatgctggct atgagcaggt caccaatgaa gacctcctga ccaactgcac gctattgctg
1141 tgccgccggc tgctgtcacc catgaacctg ctgtctctgc acacggcttc ggggctgcgc
1201 ctcttctctg tgctcagcct ggcctggggc ttcattgctg atgtggacct agagagtgag
1261 aagtatcggc gtctggggga gatgcgcttc actctgggca ccttcctgcg tctggcagcc
1321 ctgcgcacct accgcggccg actggcctac ctccctgtag gaagagtggg ttccaagaca
1381 cctgcctccc ccgttgtggt ccagcagggc ccggtagatg cacaccttgt gccactggag
1441 gagccagtgc cctctcactg gacagtggtg cccgacgagg actttgtgct agtcctggca
1501 ctgctgcact cgcacctggg cagtgagatg tttgctgcac ccatgggccg ctgtgcagct
1561 ggcgtcatgc atctgttcta cgtgcgggcg ggagtgtctc gtgccatgct gctgcgcctc
1621 ttcctggcca tggagaaggg caggcatatg gagtatgaat gcccctactt ggtatatgtg
1681 cccgtggtcg ccttccgctt ggagcccaag gatgggaaag gtgtgtttgc agtggatggg
1741 gaattgatgg ttagcgaggc cgtgcagggc caggtgcacc caaactactt ctggatggtc
1801 agcggttgcg tggagccccc gcccagctgg aagccccagc agatgccacc gccagaagag
1861 cccttatgac ccctgggccg cgctgtgcct tagtgtctac ttgcaggacc cttcctcctt
1921 ccctagggct gcagggcctg tccacagctc ctgtgggggt ggaggagact cctctggaga
1981 agggtgagaa ggtggaggct atgctttggg gggacaggcc agaatgaagt cctgggtcag
2041 gagcccagct ggctgggccc agctgcctat gtaaggcctt ctagtttgtt ctgagacccc
2101 caccccacga accaaatcca aataaagtga cattcccaaa aaaaaaaaaa aaaaaaaaaa
2161 aaaaaaaaaa aaaa
//