LOCUS BC028566 3513 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens lin-28 homolog (C. elegans), mRNA (cDNA clone
MGC:15037 IMAGE:3841184), complete cds.
ACCESSION BC028566
VERSION BC028566.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3513)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3513)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC028566.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 25 Row: a Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34222338
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..3513
/db_xref="H-InvDB:HIT000040568"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15037 IMAGE:3841184"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3513
/gene="LIN28"
/gene_synonym="CSDD1"
/gene_synonym="LIN-28"
/gene_synonym="LIN28A"
/gene_synonym="ZCCHC1"
/db_xref="GeneID:79727"
/db_xref="HGNC:HGNC:15986"
/db_xref="MIM:611043"
CDS 115..744
/gene="LIN28"
/gene_synonym="CSDD1"
/gene_synonym="LIN-28"
/gene_synonym="LIN28A"
/gene_synonym="ZCCHC1"
/codon_start=1
/product="LIN28 protein"
/protein_id="AAH28566.1"
/db_xref="GeneID:79727"
/db_xref="HGNC:HGNC:15986"
/db_xref="MIM:611043"
/translation="MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGIC
KWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAK
GLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKK
CHFCQSISHMVASCPLKAQQGPSAQGKPTYFREEEEEIHSPTLLPEAQN"
BASE COUNT 880 a 805 c 871 g 957 t
ORIGIN
1 gtgcggggga agatgtagca gcttcttctc cgaaccaacc ctttgccttc ggacttctcc
61 ggggccagca gccgcccgac caggggcccg gggccacggg ctcagccgac gaccatgggc
121 tccgtgtcca accagcagtt tgcaggtggc tgcgccaagg cggcagaaga ggcgcccgag
181 gaggcgccgg aggacgcggc ccgggcggcg gacgagcctc agctgctgca cggtgcgggc
241 atctgtaagt ggttcaacgt gcgcatgggg ttcggcttcc tgtccatgac cgcccgcgcc
301 ggggtcgcgc tcgacccccc agtggatgtc tttgtgcacc agagtaagct gcacatggaa
361 gggttccgga gcttgaagga gggtgaggca gtggagttca cctttaagaa gtcagccaag
421 ggtctggaat ccatccgtgt caccggacct ggtggagtat tctgtattgg gagtgagagg
481 cggccaaaag gaaagagcat gcagaagcgc agatcaaaag gagacaggtg ctacaactgt
541 ggaggtctag atcatcatgc caaggaatgc aagctgccac cccagcccaa gaagtgccac
601 ttctgccaga gcatcagcca tatggtagcc tcatgtccgc tgaaggccca gcagggccct
661 agtgcacagg gaaagccaac ctactttcga gaggaagaag aagaaatcca cagccctacc
721 ctgctcccgg aggcacagaa ttgagccaca atgggtgggg gctattcttt tgctatcagg
781 aagtttcgag gagcaggcag agtggagaaa gtgggaatag ggtgcattgg ggctagttgg
841 cactgccatg tatctcaggc ttgggttcac accatcaccc tttcttccct ctaggtgggg
901 ggaaagggtg agtcaaagga actccaacca tgctctgtcc aaatgcaagt gagggttctg
961 ggggcaacca ggagggggga atcaccctac aacctgcata ctttgagtct ccatccccag
1021 aatttccagc ttttgaaagt ggcctggata gggaagttgt tttcctttta aagaaggata
1081 tataataatt cccatgccag agtgaaatga ttaagtataa gaccagattc atggagccaa
1141 gccactacat tctgtggaag gagatctctc aggagtaagc attgtttttt tttcacatct
1201 tgtatcctca tacccacttt tgggataggg tgctggcagc tgtcccaagc aatgggtaat
1261 gatgatggca aaaagggtgt ttgggggaac agctgcagac ctgctgctct atgctcaccc
1321 ccgccccatt ctgggccaat gtgattttat ttatttgctc ccttggatac tgcaccttgg
1381 gtcccacttt ctccaggatg ccaactgcac tagctgtgtg cgaatgacgt atcttgtgca
1441 ttttaacttt ttttccttaa tataaatatt ctggttttgt atttttgtat attttaatct
1501 aaggccctca tttcctgcac tgtgttctca ggtacatgag caatctcagg gatagccagc
1561 agcagctcca ggtctgcgca gcaggaatta ctttttgttg tttttgccac cgtggagagc
1621 aactatttgg agtgcacagc ctattgaact acctcatttt tgccaataag agctggcttt
1681 tctgccatag tgtcctcttg aaaccccctc tgccttgaaa atgttttatg ggagaccagg
1741 ttttaactgg gtggccccat gacttgattg ccttctactg gaagattggg aattagtcta
1801 aacaggaaat ggtggtacac agaggctagg agaggctggg cccggtgaaa aggccagaga
1861 gcaagccaag attaggtgag ggttgtctaa tcctatggca caggacgtgc tttacatctc
1921 cagatctgtt cttcaccaga ttaggttagg cctaccatgt gccacagggt gtgtgtgtgt
1981 ttgtaaaact agagttgcta aggataagtt taaagaccaa tacccctgta cttaatcctg
2041 tgctgtcgag ggatggatat atgaagtaag gtgagatcct taacctttca aaattttcgg
2101 gttccaggga gacacacaag cgagggtttt gtggtgcctg gagcctgtgt cctgccctgc
2161 tacagtagtg attaatagtg tcatggtagc taaaggagaa aaagggggtt tcgtttacac
2221 gctgtgagat caccgcaaac ctaccttact gtgttgaaac gggacaaatg caatagaacg
2281 cattgggtgg tgtgtgtctg atcctgggtt cttgtctccc ctaaatgctg ccccccaagt
2341 tactgtattt gtctgggctt tgtaggactt cactacgttg attgctaggt ggcctagttt
2401 gtgtaaatat aatgtattgg tctttctccg tgttctttgg gggttttgtt tacaaacttc
2461 tttttgtatt gagagaaaaa tagccaaagc atctttgaca gaaggttctg caccaggcaa
2521 aaagatctga aacattagtt tggggggccc tcttcttaaa gtggggatct tgaaccatcc
2581 tttcttttgt attccccttc ccctattacc tattagacca gatcttctgt cctaaaaact
2641 tgtcttctac cctgccctct tttctgttca cccccaaaag aaaacttaca cacccacaca
2701 catacacatt tcatgcttgg agtgtctcca caactcttaa atgatgtatg caaaaatact
2761 gaagctagga aaaccctcca tcccttgttc ccaacctcct aagtcaagac cattaccatt
2821 tctttctttc tttttttttt tttttaaaat ggagtctcgc tgtgtcgccc aggctggagt
2881 gcagtgagct gagatcgcac cactgcactc cagcctggtt acagagcaag actctgtctc
2941 aaacaaaaca aaacaaaaca aaaacacact actgtatttt ggatggatca aacctcctta
3001 attttaattt ctaatcctaa agtaaagaga tgcaattggg ggccttccat gtagaaagtg
3061 gggtcaggag gccaagaaag ggaatatgaa tgtatatcca agtcactcag gaacttttat
3121 gcaggtgcta gaaactttat gtcaaagtgg ccacaagatt gtttaatagg agacgaacga
3181 atgtaactcc atgtttactg ctagaaacca aagctttgtg taaaatcttg aatttatggg
3241 gcgggagggt aggaaagcct gtacctgtct gtttttttcc tgatcctttt ccctcattcc
3301 tgaactgcag gagactgagc ccctttgggc tttggtgacc ccatcactgg ggtgtgttta
3361 tttgatggtt gattttgctg tactgggtac ttcctttccc attttctaat cattttttaa
3421 cacaagctga ctcttccctt cccttctcct ttccctggaa aaatacaatg aataaataaa
3481 gacttattgg tactcaaaaa aaaaaaaaaa aaa
//