LOCUS BC013051 2805 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens LIM domain kinase 2, mRNA (cDNA clone MGC:8917
IMAGE:3861412), complete cds.
ACCESSION BC013051
VERSION BC013051.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2805)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2805)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 21 Row: f Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 8051617.
FEATURES Location/Qualifiers
source 1..2805
/db_xref="H-InvDB:HIT000036105"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8917 IMAGE:3861412"
/tissue_type="Ovary, adenocarcinoma"
/clone_lib="NIH_MGC_66"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2805
/gene="LIMK2"
/db_xref="GeneID:3985"
/db_xref="HGNC:HGNC:6614"
/db_xref="MIM:601988"
CDS 231..2291
/gene="LIMK2"
/codon_start=1
/product="LIM domain kinase 2"
/protein_id="AAH13051.1"
/db_xref="GeneID:3985"
/db_xref="HGNC:HGNC:6614"
/db_xref="MIM:601988"
/translation="MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYW
GKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGK
CHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQ
VKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDP
VSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPS
SPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGK
VMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTL
KDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFG
LSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDI
FSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLE
PESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDC
QEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLST
PQKK"
BASE COUNT 665 a 754 c 792 g 594 t
ORIGIN
1 gggaggagcc atcccagcca tgagcccctg tgggaatctg ctgggggcca agtggcctgg
61 agtcctcagg ctcccgcagc tgctccggag ggagaggtga gctcagggca gcctgcctgc
121 agccagaggt gccgggagcc ccgggcctgt catggtggcc atctacagcc ggcctgaggc
181 agtcacagac ggatttgcag ctgagcctgt ctatctggtg tgggaagaag atggggagtt
241 acttgtcagt cccggcttac ttcacctcca gagacctgtt tcggtgttca gaatgccagg
301 attccctcac caactggtac tatgagaagg atgggaagct ctactgcccc aaggactact
361 gggggaagtt tggggagttc tgtcatgggt gctccctgct gatgacaggg ccttttatgg
421 tggctgggga gttcaagtac cacccagagt gctttgcctg tatgagctgc aaggtgatca
481 ttgaggatgg ggatgcatat gcactggtgc agcatgccac cctctactgt gggaagtgcc
541 acaatgaggt ggtgctggca cccatgtttg agagactctc cacagagtct gttcaggagc
601 agctgcccta ctctgtcacg ctcatctcca tgccggccac cactgaaggc aggcggggct
661 tctccgtgtc cgtggagagt gcctgctcca actacgccac cactgtgcaa gtgaaagagg
721 tcaaccggat gcacatcagt cccaacaatc gaaacgccat ccaccctggg gaccgcatcc
781 tggagatcaa tgggaccccc gtccgcacac ttcgagtgga ggaggtggag gatgcaatta
841 gccagacgag ccagacactt cagctgttga ttgaacatga ccccgtctcc caacgcctgg
901 accagctgcg gctggaggcc cggctcgctc ctcacatgca gaatgccgga cacccccacg
961 ccctcagcac cctggacacc aaggagaatc tggaggggac actgaggaga cgttccctaa
1021 ggcgcagtaa cagtatctcc aagtcccctg gccccagctc cccaaaggag cccctgctgt
1081 tcagccgtga catcagccgc tcagaatccc ttcgttgttc cagcagctat tcacagcaga
1141 tcttccggcc ctgtgaccta atccatgggg aggtcctggg gaagggcttc tttgggcagg
1201 ctatcaaggt gacacacaaa gccacgggca aagtgatggt catgaaagag ttaattcgat
1261 gtgatgagga gacccagaaa acttttctga ctgaggtgaa agtgatgcgc agcctggacc
1321 accccaatgt gctcaagttc attggtgtgc tgtacaagga taagaagctg aacctcctga
1381 cagagtacat tgaggggggc acactgaagg actttctgcg cagtatggat ccgttcccct
1441 ggcagcagaa ggtcaggttt gccaaaggaa tcgcctccgg aatggcctat ttgcactcta
1501 tgtgcatcat ccaccgggat ctgaactcgc acaactgcct catcaagttg gacaagactg
1561 tggtggtggc agactttggg ctgtcacggc tcatagtgga agagaggaaa agggccccca
1621 tggagaaggc caccaccaag aaacgcacct tgcgcaagaa cgaccgcaag aagcgctaca
1681 cggtggtggg aaacccctac tggatggccc ctgagatgct gaacggaaag agctatgatg
1741 agacggtgga tatcttctcc tttgggatcg ttctctgtga gatcattggg caggtgtatg
1801 cagatcctga ctgccttccc cgaacactgg actttggcct caacgtgaag cttttctggg
1861 agaagtttgt tcccacagat tgtcccccgg ccttcttccc gctggccgcc atctgctgca
1921 gactggagcc tgagagcaga gccccccccg gggccgcagg agagggcccg ggctgcgcgg
1981 atgatgaggg cccagtgagg cgccaaggga aggtcaccat caagtatgac cccaaggagc
2041 tacggaagca cctcaaccta gaggagtgga tcctggagca gctcacgcgc ctctacgact
2101 gccaggaaga ggagatctca gaactagaga ttgacgtgga tgagctcctg gacatggaga
2161 gtgacgatgc ctgggcttcc agggtcaagg agctgctggt tgactgttac aaacccacag
2221 aggccttcat ctctggcctg ctggacaaga tccgggccat gcagaagctg agcacacccc
2281 agaagaagtg agggtccccg acccaggcga acggtggctc ccataggaca atcgctaccc
2341 cccgacctcg tagcaacagc aataccgggg gaccctgcgg ccaggcctgg ttccatgagc
2401 agggctcctc gtgcccctgg cccaggggtc tcttcccctg ccccctcagt tttccacttt
2461 tggatttttt tattgttatt aaactgatgg gactttgtgt ttttatattg actctgcggc
2521 acgggccctt taataaagcg aggtagggta cgcctttggt gcagctcaaa aaaaaaaaaa
2581 aaaatgattt ccagcggtcc acattagagt tgaaattttc tggtgggaga atctatacct
2641 tgttccttta taggccaagg accgcagtcc ttcagtaaca ccagtgtaaa agcttgagga
2701 gaaattgtga agctacacag tatttgtttt ctaatacctc ttgtcattct aaatatcttt
2761 aatttattaa aaaatatata tatacagtaa aaaaaaaaaa aaaaa
//