LOCUS BC012075 2593 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens notchless homolog 1 (Drosophila), mRNA (cDNA clone
MGC:19970 IMAGE:4652713), complete cds.
ACCESSION BC012075
VERSION BC012075.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2593)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2593)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 29 Row: o Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62234460.
FEATURES Location/Qualifiers
source 1..2593
/db_xref="H-InvDB:HIT000035638"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19970 IMAGE:4652713"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2593
/gene="NLE1"
/gene_synonym="FLJ10458"
/gene_synonym="Nle"
/db_xref="GeneID:54475"
/db_xref="HGNC:HGNC:19889"
CDS 14..1471
/gene="NLE1"
/gene_synonym="FLJ10458"
/gene_synonym="Nle"
/codon_start=1
/product="notchless homolog 1 (Drosophila)"
/protein_id="AAH12075.1"
/db_xref="GeneID:54475"
/db_xref="HGNC:HGNC:19889"
/translation="MAAAVADEAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRL
QLVCNALLAQEDPLPLAFFVHDAEIVSSLGKTLESQAVETEKVLDIIYQPQAIFRVRA
VTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKGHRHWVL
SISWSPDGKKLASGCKNGQILLWDPSTGKQVGRTLAGHSKWITGLSWEPLHANPECRY
VASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHD
GVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYN
LVRGQGPERLVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASF
DKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVWDVKAQKLAM
DLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR"
BASE COUNT 582 a 734 c 749 g 528 t
ORIGIN
1 gtggggacgc aggatggcgg cagcagtggc ggacgaggcg gtggcgcgcg atgtgcagcg
61 gttgctagtg cagttccagg atgagggcgg gcagctgctg ggttccccgt tcgacgtgcc
121 cgtggacatc accccggaca ggctgcagct cgtgtgcaac gcgctactgg cccaggagga
181 tcccctgcca ctggctttct ttgtccacga tgctgagatc gtctcctcac tggggaagac
241 gttggagtcc caggcagtgg agacagagaa ggtcctagac atcatctacc agccacaggc
301 tatcttcaga gtccgggctg tgactcgctg caccagctcc ttggagggtc acagtgaggc
361 agtcatttct gtggccttca gccctacggg aaagtacctg gccagtggct ctggagacac
421 caccgtgcgc ttctgggatc tcagcacaga gacaccacat ttcacatgca agggacacag
481 acactgggtc cttagtatat cctggtctcc agatggcaag aagctggcct caggctgcaa
541 gaatggccag attctcctct gggacccaag cacagggaag caggtgggca ggaccctcgc
601 tggccacagc aagtggatca caggcctgag ctgggagccc ctccatgcga accctgagtg
661 ccgctatgtg gccagcagct ccaaggatgg cagtgtgcgg atctgggaca caactgcagg
721 ccgctgtgag cgcatcctca ccgggcacac ccagtcggtc acctgtctcc ggtggggagg
781 ggacgggctt ctctactctg cctcccagga ccgcaccatc aaagtctgga gagctcatga
841 cggtgtgctg tgccggactc tgcaaggcca cggccactgg gtgaacacca tggccctcag
901 cactgactat gccctgcgca ctggggcctt tgaacctgct gaggcctcag ttaatcccca
961 agacctccaa ggatccttgc aggagttgaa ggagagggct ctgagccgat acaacctcgt
1021 gcggggccag ggtccagaga ggctggtgtc tggctccgac gacttcacct tattcctgtg
1081 gtccccagca gaggacaaaa agcctctcac tcggatgaca ggacaccaag ctctcatcaa
1141 ccaggtgctc ttctctcctg actcccgcat cgtggctagt gcctcctttg acaagtccat
1201 caagctgtgg gatggcagga cgggcaagta cctggcttcc ctacgcggcc acgtggctgc
1261 cgtgtaccag attgcgtggt cagctgacag tcggctcctg gtcagcggca gcagtgacag
1321 cacactgaag gtgtgggatg tgaaggccca gaagctggcc atggacctgc ccggccacgc
1381 ggatgaggta tatgctgttg actggagtcc agatggccag agagtggcaa gtggtgggaa
1441 ggacaaatgc ctccggatat ggaggagatg agacggcccg aagttctctc tgacccccac
1501 ctcgactcgg cctctgccag ctgccttccc tgccagagaa caaaggctga gatggcagtg
1561 cacacaccct ccccaccagt ggggacctga gaatgcgtgt ggcctgctgt cctcgataga
1621 ccggaatggg gttttcccac agatccccgc ctgtggcaca ccccagagcc agaaatcgaa
1681 ggtcacagga agttgtcact gaacttggcc cgtgtctgct actctgtacc ttgctggtac
1741 agacaggggt ggtgggcagc caggctctat gagtgggccc ctagtgtcag ctctgtacag
1801 ggtcagatcc caggttctat gaccaaataa gtaacttaag ttttgtgtgt tgggttctaa
1861 ttccttgtcc tagaatcccc atgactcaat caaggactgt gctaaatgag attgtccagc
1921 ccccgccctt gcactggact acgccaaaac cacactgacc aggcacttgc cttccctctc
1981 ttcccccgtg ttggtaagag agaggccagt tgtgatagtg gccaaggaga atctagggct
2041 gtattgttgt ccactgcagt aggcaccggc cacatgtgac tgctggcatg aaatagaagt
2101 gcagttcctc catcgcactg ggtaaggcct ccagtattgg acagcacaca gaaaggtttt
2161 catcatcaag agagttctgc tggtcagccc tgctccaggg gatgcctctg ccttcgcata
2221 gcacactgct tgaggccctg ccaggcacca agcactgccc tgggcccatg ggatagagcg
2281 gggaaggtga tggctcttcc agaggattcc ctcagatggg gaggcagcag tatgagctct
2341 gagcagaagt gggtattgtt gatacagagg aagttctttg ccacgagaac tttcaagcag
2401 tgaaaggaat tcccatcagg actcagaccc caggccgaga tcttgccctg aatgtaccct
2461 gcctctgctt tctcctgcat cccatgctaa gcagggtcat ggtctgaact actcagattg
2521 gatttccaaa ccatccttgt ataaactgct cagaactaaa aaaaaaaaaa aaaaaaaaaa
2581 aaaaaaaaaa aaa
//