LOCUS BC010175 728 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2A (RAD6 homolog), mRNA
(cDNA clone MGC:20175 IMAGE:3051041), complete cds.
ACCESSION BC010175
VERSION BC010175.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 728)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 728)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC010175.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: a Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32967275.
FEATURES Location/Qualifiers
source 1..728
/db_xref="H-InvDB:HIT000034854"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20175 IMAGE:3051041"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..728
/gene="UBE2A"
/gene_synonym="HHR6A"
/gene_synonym="RAD6A"
/gene_synonym="UBC2"
/db_xref="GeneID:7319"
/db_xref="HGNC:HGNC:12472"
/db_xref="MIM:312180"
CDS 139..597
/gene="UBE2A"
/gene_synonym="HHR6A"
/gene_synonym="RAD6A"
/gene_synonym="UBC2"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2A (RAD6 homolog)"
/protein_id="AAH10175.1"
/db_xref="GeneID:7319"
/db_xref="HGNC:HGNC:12472"
/db_xref="MIM:312180"
/translation="MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPE
GTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDV
SSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC"
BASE COUNT 201 a 193 c 161 g 173 t
ORIGIN
1 cgacttcgga gaggcagcgc ggttcctctg ggtgcttccg cctccccttc tcctgcttct
61 ccagcctctt cggcctcctc gcccgccgcg ggaacccgag accccagtgt atgccccacc
121 cctgaccccg ctcgcgacat gtccaccccg gctcggcggc gcctcatgcg ggacttcaag
181 aggttgcagg aggatcctcc agccggagtc agcggggctc cgtccgagaa caacataatg
241 gtgtggaacg cggtcatttt cgggcctgaa gggaccccgt ttgaggatgg aacatttaaa
301 cttacaatag aattcactga agaatatcca aataaaccac ctacagttag atttgtctct
361 aagatgttcc atccaaatgt ctatgcagat ggtagtatat gtctggacat acttcagaac
421 cgttggagtc caacctatga tgtgtcttcc attctaacat ccatacagtc tctgttggat
481 gaacccaatc ccaatagtcc agcaaacagc caggctgctc agctgtacca ggagaacaaa
541 cgggaatatg aaaagcgtgt ttctgcaata gtagaacaaa gctggcgtga ttgttgaccc
601 cgggtacagt ttaaagaagc tggccataag aaaaatatat attgatgtgt ttgtcacctc
661 cctactcctg tcattacatt tactttatta aaagcaaaat aactgttaaa aaaaaaaaaa
721 aaaaaaaa
//