LOCUS BC000491 1271 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens proliferating cell nuclear antigen, mRNA (cDNA clone
MGC:8367 IMAGE:2820036), complete cds.
ACCESSION BC000491
VERSION BC000491.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1271)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1271)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC000491.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: b Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33239450.
FEATURES Location/Qualifiers
source 1..1271
/db_xref="H-InvDB:HIT000029674"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8367 IMAGE:2820036"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1271
/gene="PCNA"
/gene_synonym="MGC8367"
/db_xref="GeneID:5111"
/db_xref="HGNC:HGNC:8729"
/db_xref="MIM:176740"
CDS 146..931
/gene="PCNA"
/gene_synonym="MGC8367"
/codon_start=1
/product="proliferating cell nuclear antigen"
/protein_id="AAH00491.1"
/db_xref="GeneID:5111"
/db_xref="HGNC:HGNC:8729"
/db_xref="MIM:176740"
/translation="MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSH
VSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALV
FEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVI
SCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKA
TPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS"
BASE COUNT 371 a 270 c 277 g 353 t
ORIGIN
1 gcgtagcaga gtggtcgttg tctttctagg tctcagccgg tcgtcgcgac gttcgcccgc
61 tcgctctgag gctcctgaag ccgaaaccag ctagactttc ctccttcccg cctgcctgta
121 gcggcgttgt tgccactccg ccaccatgtt cgaggcgcgc ctggtccagg gctccatcct
181 caagaaggtg ttggaggcac tcaaggacct catcaacgag gcctgctggg atattagctc
241 cagcggtgta aacctgcaga gcatggactc gtcccacgtc tctttggtgc agctcaccct
301 gcggtctgag ggcttcgaca cctaccgctg cgaccgcaac ctggccatgg gcgtgaacct
361 caccagtatg tccaaaatac taaaatgcgc cggcaatgaa gatatcatta cactaagggc
421 cgaagataac gcggatacct tggcgctagt atttgaagca ccaaaccagg agaaagtttc
481 agactatgaa atgaagttga tggatttaga tgttgaacaa cttggaattc cagaacagga
541 gtacagctgt gtagtaaaga tgccttctgg tgaatttgca cgtatatgcc gagatctcag
601 ccatattgga gatgctgttg taatttcctg tgcaaaagac ggagtgaaat tttctgcaag
661 tggagaactt ggaaatggaa acattaaatt gtcacagaca agtaatgtcg ataaagagga
721 ggaagctgtt accatagaga tgaatgaacc agttcaacta acttttgcac tgaggtacct
781 gaacttcttt acaaaagcca ctccactctc ttcaacggtg acactcagta tgtctgcaga
841 tgtacccctt gttgtagagt ataaaattgc ggatatggga cacttaaaat actacttggc
901 tcccaagatc gaggatgaag aaggatctta ggcattctta aaattcaaga aaataaaact
961 aagctctttg agaactgctt ctaagatgcc agcatatact gaagtctttt ctgtcaccaa
1021 atttgtacct ctaagtacat atgtagatat tgttttctgt aaataaccta tttttttctc
1081 tattctctgc aatttgttta aagaataaag tccaaagtca gatctggtct agttaaccta
1141 gaagtatttt tgtctcttag aaatacttgt gatttttata atacaaaagg gtcttgactc
1201 taaatgcagt tttaagaatt gtttttgaat ttaaataaag ttacttgaat ttcaaaaaaa
1261 aaaaaaaaaa a
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