LOCUS AK056310 2251 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31748 fis, clone NT2RI2007384, highly similar
to Homo sapiens sterile alpha motif and leucine zipper containing
kinase AZK (ZAK), transcript variant 2, mRNA.
ACCESSION AK056310
VERSION AK056310.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2251)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2251
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2007384"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012924"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 202..1569
/codon_start=1
/note="highly similar to Homo sapiens sterile alpha motif
and leucine zipper containing kinase AZK (ZAK),
transcript variant 2, mRNA"
/protein_id="BAG51674.1"
/translation="MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAV
KKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEM
DMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNH
TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLV
VEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSF
LHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPL
AARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKA
GAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDND
MDNSE"
BASE COUNT 672 a 455 c 531 g 593 t
ORIGIN
1 gactgtcttc ctcattggcg ccgtgcagag aggcggaatg ttcaactcct aactgcggcg
61 gaaacgtggg agccgcgcgg gccgctgtcg tcccaacccc cgccgccctc gtcgcgcgcg
121 gggcctccgc gcccccggct gctgctcacg ccccgcccgg gagccagatt ttgtggaagt
181 ataatacttt gtcattatga gatgtcgtct ctcggtgcct cctttgtgca aattaaattt
241 gatgacttgc agttttttga aaactgcggt ggaggaagtt ttgggagtgt ttatcgagcc
301 aaatggatat cacaggacaa ggaggtggct gtaaagaagc tcctcaaaat agagaaagag
361 gcagaaatac tcagtgtcct cagtcacaga aacatcatcc agttttatgg agtaattctt
421 gaacctccca actatggcat tgtcacagaa tatgcttctc tgggatcact ctatgattac
481 attaacagta acagaagtga ggagatggat atggatcaca ttatgacctg ggccactgat
541 gtagccaaag gaatgcatta tttacatatg gaggctcctg tcaaggtgat tcacagagac
601 ctcaagtcaa gaaacgttgt tatagctgct gatggagtat tgaagatctg tgactttggt
661 gcctctcggt tccataacca tacaacacac atgtccttgg ttggaacttt cccatggatg
721 gctccagaag ttatccagag tctccctgtg tcagaaactt gtgacacata ttcctatggt
781 gtggttctct gggagatgct aacaagggag gtccccttta aaggtttgga aggattacaa
841 gtagcttggc ttgtagtgga aaaaaacgag agattaacca ttccaagcag ttgccccaga
901 agttttgctg aactgttaca tcagtgttgg gaagctgatg ccaagaaacg gccatcattc
961 aagcaaatca tttcaatcct ggagtccatg tcaaatgaca cgagccttcc tgacaagtgt
1021 aactcattcc tacacaacaa ggcggagtgg aggtgcgaaa ttgaggcaac tcttgagagg
1081 ctaaagaaac tagagcgtga tctcagcttt aaggagcagg agcttaaaga acgagaaaga
1141 cgtttaaaga tgtgggagca aaagctgaca gagcagtcca acaccccgct tctcttgcct
1201 cttgctgcaa gaatgtctga ggagtcttac tttgaatcta aaacagagga gtcaaacagt
1261 gcagagatgt catgtcagat cacagcaaca agtaacgggg agggccatgg catgaaccca
1321 agtctgcagg ccatgatgct gatgggcttt ggggatatct tctcaatgaa caaagcagga
1381 gctgtgatgc attctgggat gcagataaac atgcaagcca agcagaattc ttccaaaacc
1441 acatctaaga gaagggggaa gaaagtcaac atggctctgg ggttcagtga ttttgacttg
1501 tcagaaggtg acgatgatga tgatgatgac ggtgaggagg aggataatga catggataat
1561 agtgaatgaa agcagaaagc aaagtaataa aatcacaaat gtttggaaaa cacaaaagta
1621 acttgtttat ctcagtctgt acaaaaacag taaggaggca gaaagccaag cactgcattt
1681 ttaggccaat cacatttaca tgaccgtaat ttcttatcaa ttctactttt atttttgctt
1741 acagaaaaac ggggggagaa ttaagccaaa gaagtatatt tatgaatcag caaatgtggt
1801 gcctgattat agaaatttgt gatcctatat acaatatagg acttttaaag ttgtgacatt
1861 ctggcttttt cttttaatga atacttttta gtttgtattt gactttattt cctttattca
1921 aatcattttt aaaaacttac attttgaaca aacactctta actcctaatt gttctttgac
1981 acgtagtaat tctgtgacat actttttttt tcttatagca atacactgta atatcagaaa
2041 tggttggcct gagcaaccta gtaagacctc gtctctacta ataattaaaa aactagctgg
2101 catggtagca cacacctgta gtcccagata cttgggaggc caaggcagga ggattgcttg
2161 agacctagca atcagtcagg gctgcagtga gccatgatgg caccactgca ctctagcctg
2221 ggcaagagaa caagatcctg tctcaaaaaa c
//