LOCUS AK056291 2316 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31729 fis, clone NT2RI2006825.
ACCESSION AK056291
VERSION AK056291.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2316)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2316
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006825"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012905"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 123..2246
/codon_start=1
/protein_id="BAG51667.1"
/translation="MSPHGDGRGQAQGRAVRVGLRRSGGIRGGVAVFAAVAAVFTFTL
PPSVPGGDSGELITAAHELGVAHPPGYPLFTLVAKLAITLFPFGSIAYRVNLLCGLFG
AVAASLLFFTVFRLSGSSAGGILAAGVFSFSRLTWQWSIAAEVFSLNNLFVGLLMALT
VHFEEAATAKERSKVAKIGAFCCGLSLCNQHTIILYVLCIIPWILFQLLKKKELSLGS
LLKLSLYFSAGLLPYVHLPISSYLNHARWTWGDQTTLQGFLTHFLREEYGTFSLAKSE
IGSSMSEILLSQVTNMRTELSFNIQALAVCANICLATKDRQNPSLVWLFTGMFCIYSL
FFAWRANLDISKPLFMGVVERFWMQSNAVVAVLAGIGLAAVVSETNRVLNSNGLQCLE
WLSATLFVVYQIYSNYSVCDQRTNYVIDKFAKNLLTSMPHDAIILLRGDLPGNSLRYM
HYCEGLRPDISLVDQEMMTYEWYLPKMAKHLPGVNFPGNRWNPVEGILPSGMVTFNLY
HFLEVNKQKETFVCIGIHEGDPTWKKNYSLWPWGSCDKLVPLEIVFNPEEWIKLTKSI
YNWTEEYGRFDPSSWESVANEEMWQARMKTPFFIFNLAETAHMPSKVKAQLYAQAYDL
YKEIVYLQKEHPVNWHKNYAIACERMLRLQARDADPEVLLSETIRHFRLYSQKAPSDP
QQADILGALKHLRKELQSLRNRKNV"
BASE COUNT 581 a 527 c 551 g 657 t
ORIGIN
1 ggctcgaccc ggaagccgcc gtggccgccg cacaagctgc gctcgtctct cggctgggga
61 gctccgtgtc gcaccgggtt cttgggctgg ccgtgtcctt ctccctcggt cgccactggc
121 ccatgagtcc ccatggcgac ggcaggggcc aggcccaggg gcgggcagtc cgagtggggc
181 tgcggcgctc cgggggcatc cgcggcggcg tggcggtgtt cgccgccgtg gccgcagtgt
241 tcaccttcac cctgccccct tcggtaccgg ggggagactc cggggaactg atcacagccg
301 cacatgagct tggagttgcc catcctcctg gctatccttt gttcacgctg gtggctaaac
361 tggcaattac actgtttcct tttggttcaa ttgcctaccg cgtcaatctt ctctgtggct
421 tatttggagc agtagctgca tcattacttt ttttcaccgt tttcaggctt tctggctcat
481 ctgctggagg aatccttgct gcgggggtgt tttcattttc tcgtctaaca tggcagtggt
541 ccattgcagc agaggttttt agcttaaaca atctctttgt ggggctgctt atggctctta
601 ctgtacattt tgaggaagca gcaactgcta aggagagatc aaaggtagct aaaattggtg
661 ctttctgctg tggccttagt ttatgtaacc agcacacaat aatactctat gttttgtgca
721 taataccttg gattctcttt caacttttaa aaaagaagga actctccctg ggctctttgt
781 tgaagttgag cctgtacttc tctgctggtt tgctgcccta tgtccacctt cccatctcat
841 cttaccttaa tcacgcccgg tggacctggg gagaccagac aacactgcaa ggatttttga
901 cacattttct cagggaagaa tatggaacct tcagcctggc caaatctgaa ataggatcca
961 gtatgtctga aatactgctt tctcaagtaa caaatatgag gaccgaactc tcattcaaca
1021 tccaagccct tgcagtttgt gcaaatatat gtttagcaac aaaggacaga cagaatccat
1081 cattagtatg gctttttact ggaatgtttt gcatttattc attgttcttt gcttggagag
1141 caaatttaga tatttcaaaa ccacttttca tgggtgtggt ggaacgattc tggatgcaga
1201 gcaatgcagt agtggccgtc ctcgctggca ttggtttggc tgcagttgtg tctgagacta
1261 accgagtgct gaatagcaat gggcttcagt gtctggaatg gctttctgca actctttttg
1321 tagtttacca aatatattct aattacagtg tttgtgacca aaggaccaac tatgtgattg
1381 ataagttcgc aaagaacctt ctcacctcta tgcctcatga tgcaattatc ttactcagag
1441 gagatttgcc aggaaattct ctccgttaca tgcattactg tgaggggttg aggcctgaca
1501 tttcattagt ggatcaggaa atgatgactt acgagtggta tttacccaag atggcaaagc
1561 acctgccagg tgtcaacttt cctgggaacc ggtggaatcc tgtggaagga atattaccta
1621 gtggaatggt cacatttaat ctttatcatt ttcttgaagt aaataaacaa aaagaaacat
1681 ttgtttgcat aggaattcat gaaggcgacc caacctggaa aaagaactat tcactttggc
1741 catgggggtc ttgtgacaaa ttagttcctt tggagattgt attcaaccct gaggaatgga
1801 ttaaacttac aaaaagtatc tataactgga ccgaagaata tggaaggttt gatccatctt
1861 cttgggaatc tgtggccaat gaagaaatgt ggcaagcgag gatgaaaaca ccgttcttca
1921 tctttaacct ggcagaaact gctcacatgc cttcaaaagt gaaagctcaa ctctacgctc
1981 aagcatatga cctttataag gagattgtct atttacaaaa ggagcaccca gtgaattggc
2041 acaagaacta tgccatcgcc tgtgagcgga tgctgcgtct tcaggcaaga gatgcagatc
2101 ctgaagtgct gttatcggaa accatcagac atttccgtct gtactctcag aaagcaccga
2161 gtgacccaca gcaagctgat attttaggtg ctctaaagca cctaagaaaa gaactgcaaa
2221 gtctgagaaa taggaaaaat gtctgagaca gcaaaatatg aaaaacctgc tcatcgttca
2281 gcttccaaaa ttctgaagtc tggaagtttt tccttc
//