LOCUS AK056278 2932 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31716 fis, clone NT2RI2006565, highly similar
to WNT-3A PROTEIN PRECURSOR.
ACCESSION AK056278
VERSION AK056278.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2932)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2932
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006565"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012892"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 79..1137
/codon_start=1
/protein_id="BAB71136.1"
/translation="MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCAS
IPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVL
DKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIE
FGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCW
WSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEA
SPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWC
CYVSCQECTRVYDVHTCK"
BASE COUNT 460 a 1012 c 942 g 518 t
ORIGIN
1 agctcccagg gcccggcccc ccccggcgct cacgctctcg gggcggactc ccggccctcc
61 gcgccctccc gcgcggcgat ggccccactc ggatacttct tactcctctg cagcctgaag
121 caggctctgg gcagctaccc gatctggtgg tcgctggctg ttgggccaca gtattcctcc
181 ctgggctcgc agcccatcct gtgtgccagc atcccgggcc tggtccccaa gcagctccgc
241 ttctgcagga actacgtgga gatcatgccc agcgtggccg agggcatcaa gattggcatc
301 caggagtgcc agcaccagtt ccgcggccgc cggtggaact gcaccaccgt ccacgacagc
361 ctggccatct tcgggcccgt gctggacaaa gctaccaggg agtcggcctt tgtccacgcc
421 attgcctcag ccggtgtggc ctttgcagtg acacgctcat gtgcagaagg cacggccgcc
481 atctgtggct gcagcagccg ccaccagggc tcaccaggca agggctggaa gtggggtggc
541 tgtagcgagg acatcgagtt tggtgggatg gtgtctcggg agttcgccga cgcccgggag
601 aaccggccag atgcccgctc agccatgaac cgccacaaca acgaggctgg gcgccaggcc
661 atcgccagcc acatgcacct caagtgcaag tgccacgggc tgtcgggcag ctgcgaggtg
721 aagacatgct ggtggtcgca acccgacttc cgcgccatcg gtgacttcct caaggacaag
781 tacgacagcg cctcggagat ggtggtggag aagcaccggg agtcccgcgg ctgggtggag
841 accctgcggc cgcgctacac ctacttcaag gtgcccacgg agcgcgacct ggtctactac
901 gaggcctcgc ccaacttctg cgagcccaac cctgagacgg gctccttcgg cacgcgcgac
961 cgcacctgca acgtcagctc gcacggcatc gacggctgcg acctgctgtg ctgcggccgc
1021 ggccacaacg cgcgagcgga gcggcgccgg gagaagtgcc gctgcgtgtt ccactggtgc
1081 tgctacgtca gctgccagga gtgcacgcgc gtctacgacg tgcacacctg caagtaggca
1141 ccggccgcgg ctccccctgg acggggcggg ccctgcctga gggtgggctt ttccctgggt
1201 ggagcaggac tcccacctaa acggggcagt actcctccct gggggcggga ctcctccctg
1261 ggggtggggc tcctacctgg gggcagaact cctacctgaa ggcagggctc ctccctggag
1321 ccagtgtctc ctctctggtg gctgggctgc tcctgaatga ggcggagctc caggatgggg
1381 aggggctctg cgttggcttc tccctgggga cggggctccc ctggacagag gcggggctac
1441 agattgggcg gggcttctct tgggtgggac agggcttctc ctgcgggggc gaggcccctc
1501 ccagtaaggg cgtggctctg ggtgggcggg gcactaggta ggcttctacc tgcaggcggg
1561 gctcctcctg aaggaggcgg ggctctagga tggggcacgg ctctggggta ggctgctccc
1621 tgagggcgga gcgcctcctt aggagtgggg ttttatggtg gatgaggctt cttcctggat
1681 ggggcagagc ttctcctgac cagggcaagg ccccttccac gggggctgtg gctctgggtg
1741 ggcgtggcct gcataggctc cttcctgtgg gtggggcttc tctgggacca ggctccaatg
1801 gggcggggct tctctccgcg ggtgggactc ttccctggga accgccctcc tgattaaggc
1861 gtggcttctg caggaatccc ggctccagag caggaaattc agcccaccag ccacctcatc
1921 cccaaccccc tgtaaggttc catccacccc tgcgtcgagc tgggaaggtt ccatgaagcg
1981 agtcgggtcc ccaacccgtg cccctgggat ccgagggccc ctctccaagc gcctggcttt
2041 ggaatgctcc aggcgcgccg acgcctgtgc caccccttcc tcagcctggg gtttgaccac
2101 ccacctgacc aggggcccta cctggggaaa gcctgaaggg cctcccagcc cccaacccca
2161 agaccaagct tagtcctggg agaggacagg gacttcgcag aggcaagcga ccgaggccct
2221 cccaaagagg cccgccctgc ccgggctccc acaccgtcag gtactcctgc cagggaactg
2281 gcctgctgcg ccccaggccc cgcccgtctc tgctctgctc agctgcgccc ccttctttgc
2341 agctgcccag cccctcctcc ctgccctcgg gtctccccac ctgcactcca tccagctaca
2401 ggagagatag aagcctctcg tcccgtccct ccctttcctc cgcctgtcca cagcccctta
2461 agggaaaggt aggaagagag gtccagcccc ccaggctgcc cagagctgct ggtctcattt
2521 gggggcgttc gggaggtttg gggggcatca accccccgac tgtgctgctc gcgaaggtcc
2581 cacagccctg agatgggccg gcccccttcc tggcccctca tggcgggact ggagaaatgg
2641 tccgctttcc tggagccaat ggcccggccc ctcctgactc atccgcctgg cccgggaatg
2701 aatggggagg ccgctgaacc cacccggccc atatccctgg ttgcctcatg gccagcgccc
2761 ctcagcctct gccactgtga accggctccc accctcaagg tgcggggaga agaagcggcc
2821 aggcggggcg ccccaagagc ccaaaagagg gcacaccgcc atcctctgcc tcaaattctg
2881 cgtttttggt tttaatgtta tatctgatgc tgctatatcc actgtccaac gg
//