LOCUS AK056270 2594 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31708 fis, clone NT2RI2006271, highly similar
to Alpha-2,8-sialyltransferase 8E (EC 2.4.99.-).
ACCESSION AK056270
VERSION AK056270.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2594)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2594
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006271"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012884"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 662..1792
/codon_start=1
/note="highly similar to Alpha-2,8-sialyltransferase 8E
(EC 2.4.99.-)"
/protein_id="BAG51661.1"
/translation="MRYADPSANRDLLGSRTLLFIFICAFALVTLLQQILYGRNYIKR
YFEFYEGPFEYNSTRCLELRHEILEVKVLSMVKQSELFDRWKSLQMCKWAMNISEANQ
FKSTLSRCCNAPAFLFTTQKNTPLGTKLKYEVDTSGIYHINQEIFRMFPKDMPYYRSQ
FKKCAVVGNGGILKNSRCGREINSADFVFRCNLPPISEKYTMDVGVKTDVVTVNPSII
TERFHKLEKWRRPFYRVLQVYENASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYY
FHPQYLVNVSRYWLSLGVRAKRISTGLILVTAALELCEEVHLFGFWAFPMNPSGLYIT
HHYYDNVKPRPGFHAMPSEIFNFLHLHSRGILRVHTGTCSCC"
BASE COUNT 517 a 844 c 719 g 514 t
ORIGIN
1 ttttaattcc aagattcaaa cccggcgttt tgcgctccgc ccctccccct ccccactgcc
61 cgcctatcat tagccagact gcgcgggtgt tttgtacgcc ccctcccccc gtccctatcg
121 gcagaaccgg aggccaacct tcgcgatccc ttgctgcggg cccggagatc aaacgtggcc
181 cgcccccggc agggcacagc gcgctgggca accgcgatcc ggcgccggac tggaggggtc
241 gatgcgctgc gcgctggggc gcacagggga cggaacccgg gtcttgctcc ccattggccg
301 ccacctcggc gggaggggcc gcctccggct cgtggcacct ggcgtctccc ctccgcgggg
361 aaatcggcgg ctacccggca ccggcgcctg gaggtaaagg tacccggggg tggggaaggc
421 ccctggccgc ggcagagctc cctgccaccg gccctgtcct gcttgccgcc ccagcgcctg
481 cgacgcttgg atgcccccag ctccggccgc gccaaccaga aactttgccc ctcgaagtcc
541 cccgcctgcc tgaccgcacc gtgaggcgcc aagtcggccg cgcgcccccc ggggccccgg
601 aaccccgcga gtcattgcct ggccgccccg taccccgcgc ccgcggcgcc cggtagccag
661 gatgcgctac gcggacccct cggccaaccg ggatttgttg gggagccgaa ctttgctctt
721 catcttcatc tgcgcctttg ccttggtgac cttgctgcaa cagatcctgt atggcaggaa
781 ctacattaag aggtactttg aattttatga ggggcctttt gaatataact ccacaagatg
841 cctggagctg aggcacgaaa tattggaagt gaaggtgctg tccatggtga agcagtcaga
901 gctgttcgac aggtggaaga gcctccagat gtgcaaatgg gcgatgaaca tctctgaggc
961 caaccagttc aagtctactc tgtccaggtg ctgcaacgcc cctgcctttc tcttcaccac
1021 ccagaagaac actcccctgg ggacaaagct caagtatgag gtggacacca gtggcatcta
1081 ccacatcaac caggagatct tccgcatgtt tcccaaggac atgccctact accggtccca
1141 gtttaagaag tgtgctgtag tgggcaacgg aggcatcttg aagaacagcc gctgcgggag
1201 ggagatcaac agcgccgact tcgtcttccg gtgcaacctg ccccccatct cagagaagta
1261 caccatggat gtgggggtga agacggatgt ggtcactgtg aaccccagca tcatcacaga
1321 gaggttccac aagctggaga agtggcggcg gccgttctat cgcgtgctgc aggtgtacga
1381 gaacgcgtcg gtgctgctgc ctgccttcta caacacgcgc aacaccgacg tgtccatccg
1441 cgtcaagtac gtgctggacg acttcgaatc gccgcaagct gtctactact tccatccgca
1501 gtacctggtc aacgtgtcgc gctactggct cagcctgggg gtgcgcgcca agcgcatcag
1561 caccggcctc attctggtca ctgcggcgct ggagctctgt gaggaggtgc acctctttgg
1621 cttttgggcc ttccccatga acccctcggg cctctacatc actcaccact actatgacaa
1681 cgtcaagccg cgtcccggct tccacgccat gccctctgag atcttcaact tcctgcactt
1741 gcacagccga ggcatcctcc gcgtgcacac gggcacctgc agctgctgct gatggctgcc
1801 agccaggctg cccggcaagc ggcgccccct ctcctgctgt ccctcctggt ggaactggga
1861 gccccgaacc gggcagcgtg gggtcctggc gttcaggctg tctctccctc cattgttcag
1921 ctctgtactt ggatcctggg gtagggaggc agggttagga accggggcag agtcagctct
1981 gtgctgagcc tcctggcctg gcccccaccg gtgcatccgc ccagtgcgtc cacctgccct
2041 ggctagcatg ctgctgggcc acaccccaag atcaggggcc ctggggacgg caagtgaata
2101 aagcacattt ccacccaatt ttgtcatccg agagagagca caaactgcag gcccttgttg
2161 cagctgaagg aagaccctac agagaatgga attgagagtg gagacaggac acttcacagg
2221 acacttgagc acagtcaaga ttttattcac acttttggtt cctgtgtttt atatcgaaga
2281 cttagctatg aattgctatc taaatctcag agcttagaag ccaacccagt gactagacct
2341 tccagtgaag aaagtgatct caagaggtcc agggacttaa cagccaagcc acaccacccg
2401 acaggttctt ctgtgacacc gagagatcta acccaaggcc tgggttatgt cttagtcgag
2461 acatcatcat ctgagaagca ccacatcctt tcagacacat cctcggacac acatgcggtc
2521 ttggtgcccc caggttcaat cctgcgttag ctcttttgat gagaaggaaa taaaccaaac
2581 cagccacttg cacc
//