LOCUS AK056157 2656 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31595 fis, clone NT2RI2002517, weakly similar
to Homo sapiens PAR3 mRNA.
ACCESSION AK056157
VERSION AK056157.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2656)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2656
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2002517"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012771"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 90..1727
/codon_start=1
/protein_id="BAB71106.1"
/translation="MKVTVCFGRTGIVVPCKEGQLRVGELTQQALQRYLKTREKGPGY
WVKIHHLEYTDGGILDPDDVLADVVEDKDKLIAVFEEQEPLHKIESPSGNPADRQSPD
AFETEVAAQLAAFKPIGGEIEVTPSALKLGTPLLVRRSSDPVPGPPADTQPSASHPGG
QSLKPVVPDSTQNLEDREVLNGVQTELLTSPKTKDTLSDMTRTVEISGEGGPLGIHVV
PFFSSLSGRILGLFIRGIEDNSRSKREGLFHENECIVKINNVDLVDKTFAQAQDVFRQ
AMKSPSVLLHVLPPQNREQYEKSVIGSLNIFGNNDGVLKTKVPPPVHGKSGLKTANLT
GTDSPETDASASLQQNKSPRVPRLGGKPSSPSLSPLMGFGSNKNAKKIKIDLKKGPEG
LGFTVVTRDSSIHGPGPIFVKNILPKGAAIKDGRLQSGDRILEVNGRDVTGRTQEELV
AMLRSTKQGETASLVIARQEGHFLPRELKGEPDCCALSLETSEQLTFEIPLNDSGSAG
LGVSLKGNKSRETGTDLGILSNPSFMEALLLRMVVCE"
BASE COUNT 762 a 622 c 712 g 560 t
ORIGIN
1 ccgtcccggg cgtcctccga gagtgggggc tgcgcccgcg gggtcagaca cctgttcggc
61 ccggcccggc gtggtcgccg ggggccagga tgaaagtgac cgtgtgcttc ggcaggacgg
121 gcatcgtggt gccctgcaag gagggccagc tgcgcgtcgg cgagctcacc cagcaggcgc
181 tgcagcggta cctgaagacc cgggagaagg gtcctggtta ctgggtgaag attcatcact
241 tagaatatac agatggagga atcctggatc cagatgatgt cttggcagat gttgttgaag
301 ataaagacaa gctgattgct gtgtttgaag aacaagaacc actccacaag attgagagcc
361 ccagtggaaa ccctgcagat cggcagagcc cagatgcttt tgagacagaa gtggccgccc
421 aactggctgc atttaagcca attggtgggg agattgaagt aaccccttct gctctaaaac
481 taggcactcc actgctggtg aggagaagca gtgacccagt gccaggccca cctgctgata
541 cccagccaag cgcttcacac cctggtggcc agagtctgaa accggttgtt ccagattcca
601 cgcagaactt ggaagacaga gaagttttga atggtgtaca gacagaacta ctaacttcgc
661 caaaaactaa ggacacattg agtgatatga caagaacagt ggagatttct ggggaaggag
721 gcccattggg aatacatgta gtgcccttct tttcatctct gagtggaagg attctaggac
781 tcttcatccg aggcattgaa gacaacagca ggtccaagcg ggagggacta tttcacgaaa
841 atgaatgtat tgtaaaaatc aacaatgtgg atctcgtaga caaaaccttt gctcaggctc
901 aggatgtctt ccgccaggca atgaaatctc caagtgtgct cctccacgtg cttcctccac
961 aaaaccgtga acagtatgaa aagtcagtca ttggctctct taacattttt ggtaataatg
1021 atggcgtttt gaaaaccaaa gtgccgcctc ctgtccatgg aaaatcggga ctaaagacag
1081 caaatctcac aggaaccgat agtcctgaaa cagatgcatc agcttccctg caacaaaaca
1141 agagtccccg agtaccaagg ctgggaggaa aaccatcctc tccctcactc tcgcctctca
1201 tgggatttgg cagcaataaa aatgcaaaga aaattaagat tgacctaaag aaaggccctg
1261 aaggacttgg tttcactgtg gttaccagag actcttccat acatggtccc ggtcccattt
1321 ttgtaaaaaa cattttacca aagggagcag caataaaaga tggccgccta caatcagggg
1381 acagaatttt ggaggtaaat gggagagatg tcaccggacg aacccaggaa gagcttgtgg
1441 ccatgctcag gagcaccaag cagggggaga cagcatcgct ggtcattgcc cgccaagaag
1501 gacattttct gccccgagag ttgaaaggag aacctgactg ctgtgcactc tctctggaga
1561 caagcgagca gctcaccttt gagatccccc tgaatgattc aggttctgct ggcctcgggg
1621 tgagcttaaa agggaacaaa tccagagaaa ctggaacaga cttggggatt ttatcaaatc
1681 catcattcat ggaggcgctg cttttaagga tggtcgtctg cgaatgaatg accagctgat
1741 tgcagttaat ggggaatctc ttttgggaaa gtccaaccac gaagctatgg aaacacttag
1801 gcggtcaatg tccatggagg gaaacatccg agggatgatc cagttggtga ttctgaggag
1861 gccagagaga ccaatggagg atcctgcaga gtgtggggca ttttccaagc catgctttga
1921 gaactgtcaa aatgctgtaa ccacctctag gcgaaatgat aatagtatcc tgcatccact
1981 tggcacttgc agtccacaag acaaacagaa aggtctattg ctgcccaatg acggatgggc
2041 cgagagtgaa gttccacctt ctccaacacc acattctgct ctgggattgg gcctcgaaga
2101 ttacagccac agctctgggg tggattcagc agtatatttt ccagatcagc acatcaactt
2161 cagatctgtg acaccggcca ggcagcctga atcaattaat ttgaaagcct cgaagagcat
2221 ggaccttgtg ccagatgaaa gcaaggttca ctcattggct ggacaaaaat cggaatctcc
2281 aagcaaagat tttggtccaa ctctgggttt gaaaaagtcc agctccttgg agagtctgca
2341 gactgcagtg gccgaggtca ggaagaatga ccttcccttt cacaggcccc ggccgcacat
2401 ggttcgaggc cgaggctgca atgagagctt tagagcagcc attgacaaat cctacgatgg
2461 acctgaagaa atagaagctg acggtctgtc tgataagagc tctcactctg gccaaggagc
2521 tctgaattgt gagtctgccc ctcaggggaa ttcggagcta gaggacatgg aaaataaagc
2581 caggaaagtc aaaaaaacga aagagaagga gaagaaaaag gaaaagggca aattgaaagt
2641 caaggagaaa aagcgc
//