LOCUS AK056122 1844 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31560 fis, clone NT2RI2001409, weakly similar
to DYSTROPHIN.
ACCESSION AK056122
VERSION AK056122.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1844)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1844
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2001409"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012736"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 57..1739
/codon_start=1
/protein_id="BAB71097.1"
/translation="MHTEMQALRADWKQWEDSVFQTQSCLENLVSQMALSEQEFSGQV
AQLEQALEQFSALLKTWAQQLTLLEGKNTDEEIVECWHKGQEILDALQKAEPRTEDLK
SQLNELCRFSRDLSTYSGKVSGLIKEYNCLCLQASKGCQNKEQILQQRFRKAFRDFQQ
WLVNAKITTAKCFDIPQDISEVSTSLQKIQEFLSESENGQHKLNMMLSKGELLSTLLT
KEKAKGIQAKVTAAKEDWKNFHSNLHQKESALENLKIQMKDFEVSAEPIQDWLSKTEK
MVHESSNRLYDLPAKRREQQKLQSVLEEIHCYEPQLNRLKEKAQQLWEGQAASKSFRH
RVSQLSSQYLALSNLTKEKVSRLDRIVAEHNQFSLGIKELQDWMTDAIHMLDSYCHPT
SDKSVLDSRTLKLEALLSVKQEKEIQMKMIVTRGESVLQNTSPEGIPTIQQQLQSVKD
MWASLLSAGIRCKSQLEGALSKWTSYQDGVRQFSGWMDSMEANLNESERQHAELRDKT
TMLGKAKLLNEEVLSYSSLLETIEVKGAGMTEHYVTQLELQDLQERYRAIHR"
BASE COUNT 586 a 409 c 460 g 389 t
ORIGIN
1 tggagtcgct ggctcccgaa gtgaaacaga acacaactgc cagtgggtgt gagctcatgc
61 acacggagat gcaggccctg cgtgccgact ggaagcagtg ggaagacagt gtattccaaa
121 cgcagagctg tttggagaac ctggtcagcc agatggccct ttcggagcag gaattctcag
181 gccaagtggc tcaactggag caggccctgg aacagttcag tgcccttctg aaaacctggg
241 ctcagcagtt aaccctcctg gaaggcaaga acacggatga ggagatagtg gaatgctggc
301 acaaaggaca agagatactg gatgctttgc aaaaagcaga gcctagaaca gaggatctca
361 agtctcagct gaatgaactt tgtcgatttt ccagagacct gagtacctac agtggaaaag
421 tttctggctt gattaaagag tataattgtc tttgtttgca agcatccaag ggctgccaga
481 ataaagaaca gattttacag caaagatttc gaaaggcctt cagggatttc cagcagtggt
541 tggttaatgc aaaaatcact accgccaagt gttttgatat acctcaagat ataagtgaag
601 tttcaactag tcttcagaaa atacaggagt ttttgtcaga aagtgaaaat ggacagcaca
661 agctaaacat gatgctgtct aaaggggaac ttctgagtac cctgctgacc aaagagaaag
721 cgaaagggat ccaggccaaa gttacagctg caaaagaaga ttggaaaaat tttcattcaa
781 atctccacca aaaagagtct gctctagaga atctaaagat ccaaatgaag gactttgaag
841 taagtgctga gcctatccag gactggctga gtaaaactga gaagatggtc catgaaagca
901 gcaatcgcct ctatgatctg ccagcaaaga ggagggagca gcagaagctc cagtctgtcc
961 ttgaggaaat acactgctac gagcctcagc tcaacaggct gaaagagaaa gctcagcagc
1021 tgtgggaagg acaagctgcc agcaagagct ttaggcacag agtgtcgcag ctgtcttctc
1081 agtatctagc gctaagcaat ttaacaaagg agaaagtgtc aagactggat agaatcgttg
1141 cagaacacaa tcagttctct cttgggatta aagaattaca agactggatg acggatgcga
1201 ttcacatgct ggattcatac tgccacccga catccgacaa aagtgtgctg gacagcagga
1261 cgctcaagct cgaggctcta ttatcagtca aacaggaaaa agagattcag atgaaaatga
1321 tagtgaccag gggagaatct gtccttcaga atacttctcc agaaggcatt cccactattc
1381 agcagcagct gcagagtgtg aaggatatgt gggcatccct tttgtctgca gggattcgtt
1441 gtaaaagcca actcgaagga gctctctcca agtggacaag ttatcaggat ggagttcgac
1501 agttctccgg ttggatggat agtatggaag ccaacctgaa tgaatcagaa aggcagcatg
1561 cggagctgcg ggataaaaca acgatgctcg gaaaagccaa gttattaaat gaagaagtgc
1621 tgagttacag cagcttgctg gagaccatcg aagtcaaagg ggctggcatg acagaacact
1681 atgtcaccca gctagaactc caggatctac aggaacgata cagagccatc catcgctaac
1741 cccactaaaa cactcaccaa gacctcaacc cctgaccccc atgcctcagg atactcctca
1801 atagccatcg ctgtagtata tccaaagaca accatcattc cccc
//