LOCUS X76732 1586 bp mRNA linear HUM 23-OCT-2008
DEFINITION H.sapiens mRNA for NEFA protein.
ACCESSION X76732
VERSION X76732.1
KEYWORDS acute lymphoblastic leukemia; calcium-binding protein; DNA-binding
protein:nuclear localization signal; EF-hand; leucine zipper; NEFA
protein; signal peptide.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1586)
AUTHORS Barnikol-Watanabe S., Gross N., Goetz H., Henkel T., Karabinos A.,
Kratzin H., Barnikol H.U., Hilschmann N.
TITLE Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper
protein. Molecular cloning and sequence analysis of the cDNA,
isolation and characterization of the protein
JOURNAL Biol. Chem. Hoppe-Seyler 375(8), 497-512(1994).
PUBMED 7811391
REFERENCE 2
AUTHORS Barnikol H.U.
JOURNAL Submitted (15-DEC-1993) to the INSDC. H.U. Barnikol,
Max-Planck-Inst. fuer Experimentelle Medizin, Hermann-Rein-Str. 3,
37075 Goettingen, FRG
REMARK revised by [3]
REFERENCE 3 (bases 1 to 1586)
AUTHORS Barnikol H.U.
JOURNAL Submitted (18-DEC-1997) to the INSDC. H.U. Barnikol,
Max-Planck-Inst. fuer Experimentelle Medizin, Hermann-Rein-Str. 3,
37075 Goettingen, FRG
COMMENT Name: NEFA (N=DNA-binding, EF=EF-hand, A=acidic region)
Function: DNA-binding protein. The calcium-binding EF-hand
structures together with the acidic region may be
involved in regulation of the DNA-binding function
of protein NEFA
Disease: CALLA (CD10) positive human acute lymphoblastic
leukemia (ALL)
Similarity: human nucleobindin EMBL: M96824; HSNUCLEOB
GB:M96824; HUMNUCLEOB
PATCHX:M96824
SWISSPROT:Q02818; NUBN_HUMAN
murine nucleobindin EMBL:M96823; MMNUCLEOB
GB:M96823; MUSNUCLEOB
PIR:JC1224; PC1116
SWISSPROT:Q02819; NUBN_MOUSE
FEATURES Location/Qualifiers
source 1..1586
/db_xref="H-InvDB:HIT000323598"
/organism="Homo sapiens"
/mol_type="mRNA"
/sex="male"
/dev_stage="12 year old"
/clone_lib="lambda gt11"
/clone="pBluescript (Stratagene)"
/cell_line="KM-3"
/cell_type="pre-B lymphoblast"
/tissue_type="blood"
/db_xref="taxon:9606"
sig_peptide 220..294
/gene="NEFA"
/inference="non-experimental evidence, no additional
details recorded"
CDS 220..1482
/gene="NEFA"
/product="NEFA protein"
/function="DNA-binding protein"
/note="NEFA (N=DNA-binding, EF=EF-hand, A=acidic region)"
/db_xref="GOA:P80303"
/db_xref="H-InvDB:HIT000323598.14"
/db_xref="HGNC:HGNC:8044"
/db_xref="InterPro:IPR002048"
/db_xref="InterPro:IPR011992"
/db_xref="InterPro:IPR018247"
/db_xref="InterPro:IPR028813"
/db_xref="InterPro:IPR040250"
/db_xref="UniProtKB/Swiss-Prot:P80303"
/experiment="experimental evidence, no additional details
recorded"
/protein_id="CAA54148.1"
/translation="MRWRTILLQYCFLLITCLLTALEAVPIDIDKTKVQNIHPVESAK
IEPPDTGLYYDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTK
LDELKRQEVGRLRMLIKAKLDSLQDIGMDHQALLKQFDHLNHLNPDKFESTDLDMLIK
AATSDLEHYDKTRHEEFKKYEMMKEHERREYLKTLNEEKRKEEESKFEEMKKKHENHP
KVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEK
VYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVTLEEFLKATEKKEFLEPDSWE
TLDQQQFFTEEELKEYENIIALQENELKKKADELQKQKEELQRQHDQLEAQKLEYHQV
IQQMEQKKLQQGIPPSGPAGELKFEPHI"
misc_feature 232..300
/note="hydrophobic region, HY1, AA 5-27"
mat_peptide 295..1479
/gene="NEFA"
/product="NEFA protein"
/experiment="experimental evidence, no additional details
recorded"
misc_feature 703..888
/note="DNA-binding domain, DNB, AA 162-223"
/inference="non-experimental evidence, no additional
details recorded"
repeat_region 810..816
/gene="NEFA"
/rpt_type=DIRECT
/note="repeat 1.1"
misc_feature 814..822
/gene="NEFA"
/note="nuclear localization signal, NLS1, AA 199-201"
/inference="non-experimental evidence, no additional
details recorded"
repeat_region 817..823
/gene="NEFA"
/rpt_type=DIRECT
/note="repeat 2.1"
repeat_region 840..847
/gene="NEFA"
/rpt_type=DIRECT
/note="repeat 2.1"
repeat_region 848..855
/gene="NEFA"
/rpt_type=DIRECT
/note="repeat 2.2"
misc_feature 850..861
/gene="NEFA"
/note="nuclear localization signal, NLS2, AA 211-214"
/inference="non-experimental evidence, no additional
details recorded"
misc_feature 952..1038
/gene="NEFA"
/note="calcium-binding EF-hand, EF1, AA 245-273"
/inference="non-experimental evidence, no additional
details recorded"
misc_feature 1063..1098
/gene="NEFA"
/note="acidic region, AR, AA 282-293"
misc_feature 1108..1194
/gene="NEFA"
/note="calcium-binding EF-hand, EF2, AA 297-325"
/inference="non-experimental evidence, no additional
details recorded"
misc_feature 1258..1374
/gene="NEFA"
/note="leucine zipper, LEU, AA 347-382"
/inference="non-experimental evidence, no additional
details recorded"
misc_feature 1420..1455
/gene="NEFA"
/note="hydrophobioc region, HY2, AA 401-412"
regulatory 1568..1573
/regulatory_class="polyA_signal_sequence"
BASE COUNT 601 a 269 c 340 g 376 t
ORIGIN
1 gaggacaggt ttgtgcgctg gacgcaagca ccaggcgcag cctcgctcgc cgacacccgg
61 ccagaacgtg ttacgagtca gtttttagtg aaaaaacatt gagctaggag ccaagaccca
121 tctcttcact attttggtat tgtgcaagtc atcttacctc tctggatctc agttgtctca
181 tctgtaaaaa ggagataaaa attatttacc tgcctgaaca tgaggtggag gaccatcctg
241 ctacagtatt gctttctctt gattacatgt ttacttactg ctcttgaagc tgtgcctatt
301 gacatagaca agacaaaagt acaaaatatt caccctgtgg aaagtgcgaa gatagaacca
361 ccagatactg gactttatta tgatgaatat ctcaagcaag tgattgatgt gctggaaaca
421 gataaacact tcagagaaaa gctccagaaa gcagacatag aggaaataaa gagtgggagg
481 ctaagcaaag aactggattt agtaagtcac catgtgagga caaaacttga tgaactgaaa
541 aggcaagaag taggaaggtt aagaatgtta attaaagcta agttggattc ccttcaagat
601 ataggcatgg accaccaagc tcttctaaaa caatttgatc acctaaacca cctgaatcct
661 gacaagtttg aatccacaga tttagatatg ctaatcaaag cggcaacaag tgatctggaa
721 cactatgaca agactcgtca tgaagaattt aaaaaatatg aaatgatgaa ggaacatgaa
781 aggagagaat atttaaaaac attgaatgaa gaaaagagaa aagaagaaga gtctaaattt
841 gaagaaatga agaaaaagca tgaaaatcac cctaaagtta atcacccagg aagcaaagat
901 caactaaaag aggtatggga agagactgat ggattggatc ctaatgactt tgaccccaag
961 acatttttca aattacatga tgtcaatagt gatggattcc tggatgaaca agaattagaa
1021 gccctattta ctaaagagtt ggagaaagta tatgacccta aaaatgaaga ggatgatatg
1081 gtagaaatgg aagaagaaag gcttagaatg agggaacatg taatgaatga ggttgatact
1141 aacaaagaca gattggtgac tctggaggag tttttgaaag ccacagaaaa aaaagaattc
1201 ttggagccag atagctggga gacattagat cagcaacagt tcttcacaga ggaagaacta
1261 aaagaatatg aaaatattat tgctttacaa gaaaatgaac ttaagaagaa ggcagatgag
1321 cttcagaaac aaaaagaaga gctacaacgt cagcatgatc aactggaggc tcagaagctg
1381 gaatatcatc aggtcataca gcagatggaa caaaaaaaat tacaacaagg aattcctcca
1441 tcagggccag ctggagaatt gaagtttgag ccacacattt aaagtctgaa gtccaccaga
1501 acttggaaga aagctgttaa ctcaacatct atttcatctt tttagctccc ttcccttttc
1561 tctgctcaat aaatatttta aaagca
//