LOCUS X64168 324 bp mRNA linear HUM 26-JUL-1997 DEFINITION H.sapiens mRNA for RegAL kappa light chain variable Ig domain. ACCESSION X64168 VERSION X64168.1 KEYWORDS Ig kappa light chain; Ig light chain; immunoglobulin light chain variable region; light chain; variable region. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 324) AUTHORS Hughes-Jones N.C. JOURNAL Submitted (08-JAN-1992) to the INSDC. N.C. Hughes-Jones, Inst of Animal Physiology and Genetics Research, Babraham Hall, Cambridge CB2 4AT, UK REFERENCE 2 (bases 1 to 324) AUTHORS Bye J.M., Carter C., Cui Y., Gorick B.D., Songsivilai S., Winter G., Hughes-Jones N.C., Marks J.D. TITLE Germline variable region gene segment derivation of human monoclonal anti-Rh(D) antibodies. Evidence for affinity maturation by somatic hypermutation and repertoire shift JOURNAL J. Clin. Invest. 90(6), 2481-2490(1992). PUBMED 1469099 COMMENT See also X64148-69 FEATURES Location/Qualifiers source 1..324 /db_xref="H-InvDB:HIT000322667" /organism="Homo sapiens" /chromosome="2" /isolate="blood donor Re" /mol_type="mRNA" /cell_line="Reg-A" /cell_type="B-lymphocytes" /db_xref="taxon:9606" misc_feature 1..23 /note="PCR primer" misc_feature 1..324 /note="VJ region" misc_feature 1..285 /note="V kappa region" misc_feature 70..105 /note="CDR1 region" misc_feature 151..171 /note="CDR2 region" misc_feature 268..291 /note="CDR3 region" misc_feature 286..324 /note="J region" BASE COUNT 72 a 94 c 85 g 70 t ORIGIN 1 gawrttcagc tgackcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc 61 ctctcctgca gggccagtca gagtatgagc agcaactact tagcctggta ccagcagaaa 121 cctggccagg ctcccagggt cctcatctat ggtgcatcca gcagggccac tggcatccca 181 gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag 241 cctgaagatt ctgcagtgta ttactgtcag cagtttggtg gctcattcac tttcggccct 301 gggaccacag tggatatcaa acga //