LOCUS X06745 5433 bp mRNA linear HUM 07-OCT-2008
DEFINITION Human mRNA for DNA polymerase alpha-subunit.
ACCESSION X06745
VERSION X06745.1
KEYWORDS DNA polymerase; DNA polymerase alpha subunit; polymerase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5433)
AUTHORS Wong S.W., Wahl A.F., Yuan P.M., Arai N., Pearson B.E., Arai K.,
Korn D., Hunkapiller M.W., Wang T.S.
TITLE Human DNA polymerase alpha gene expression is cell proliferation
dependent and its primary structure is similar to both prokaryotic
and eukaryotic replicative DNA polymerases
JOURNAL EMBO J. 7(1), 37-47(1988).
PUBMED 3359994
COMMENT Data kindly reviewed (16-Jan-1989) by Wang T.S.F.
FEATURES Location/Qualifiers
source 1..5433
/db_xref="H-InvDB:HIT000321204"
/organism="Homo sapiens"
/mol_type="mRNA"
/clone_lib="E1 in lambda gt10"
/clone="E1-14b8, E1-14a, E1-12, E1-19"
/cell_line="KB"
/cell_type="pre-B-cell"
/db_xref="taxon:9606"
mRNA <1..5433
/note="DNA polymerase alpha mRNA"
CDS 17..4405
/note="DNA polymerase alpha-subunit (AA 1 - 1462)"
/db_xref="GOA:P09884"
/db_xref="H-InvDB:HIT000321204.14"
/db_xref="HGNC:HGNC:9173"
/db_xref="InterPro:IPR006133"
/db_xref="InterPro:IPR006134"
/db_xref="InterPro:IPR006172"
/db_xref="InterPro:IPR012337"
/db_xref="InterPro:IPR015088"
/db_xref="InterPro:IPR017964"
/db_xref="InterPro:IPR023211"
/db_xref="InterPro:IPR024647"
/db_xref="InterPro:IPR036397"
/db_xref="InterPro:IPR038256"
/db_xref="InterPro:IPR042087"
/db_xref="PDB:1K0P"
/db_xref="PDB:1K18"
/db_xref="PDB:1N5G"
/db_xref="PDB:4Q5V"
/db_xref="PDB:4QCL"
/db_xref="PDB:4Y97"
/db_xref="PDB:5EXR"
/db_xref="PDB:5IUD"
/db_xref="PDB:6AS7"
/db_xref="UniProtKB/Swiss-Prot:P09884"
/protein_id="CAA29920.1"
/translation="MAPVHGDDSLSDSGSFVSSRARREKKSKKGRQEALERLKKAKAG
EKYKYEVEDFTGVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDD
ALDADEKGKDGKARNKDKRNVKKLAVTKPNNIKSMFIACAGKKTADKAVDLSKDGLLG
DILQDLNTETPQITPPPVMILKKKRSIGASPNPFSVHTATAVPSGKIASPVSRKEPPL
TPVPLKRAEFAGDDVQVESTEEEQESGAMEFEDGDFDEPMEVEEVDLEPMAAKAWDKE
SEPAEEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLVKG
ADEEQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTLYFLPR
EMKIDLNTGKETGTPISMKDVYEEFDEKIATKYKIMKFKSKPVEKNYAFEIPDVPEKS
EYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKKSTALN
QPVSWCKVEAMALKPDLVNVIKDVSPPPLVVMAFSMKTMQNAKNHQNEIIAMAALVHH
SFALDKAAPKPPFQSHFCVVSKPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLA
KVHKIDPDIIVGHNIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGGRSGFGER
NATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLY
LLEHTWKDAKFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYE
NNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKGAYAGGLVLDPKVGFYDKFI
LLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVTEDGEQEQIPELPDPSLEMGILPRE
IRKLVERRKQVKQLMKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPL
AALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN
KLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCDLA
KDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDY
PDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTASQRAYAPEQLQKQDNL
TIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEENDALLG
GPAQLTDEEKYRDCERFKCPCPTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPL
TFTVQLSNKLIMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQFSRTGPLCPACMKAT
LQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNT
AEQFLSRSGYSEVNLSKLFAGCAVKS"
misc_feature 5415..5420
/note="polyA signal"
polyA_site 5433..5433
/note="polyA site"
BASE COUNT 1673 a 1052 c 1271 g 1437 t
ORIGIN
1 ggggagattc gggaccatgg cacctgtgca cggcgacgac tctctgtcag attcagggag
61 ttttgtatct tctcgagccc ggcgagaaaa aaaatcaaag aaggggcgcc aagaagccct
121 agaaagactg aaaaaggcta aagctggtga gaagtataaa tatgaagtcg aggacttcac
181 aggtgtttat gaagaagttg atgaagaaca gtattcgaag ctggttcagg cacgccagga
241 tgatgactgg attgtggatg atgatggtat tggctatgtg gaagatggcc gagagatttt
301 tgatgatgac cttgaagatg atgcccttga tgctgatgag aaaggaaaag atggtaaagc
361 acgcaataaa gacaagagga atgtaaagaa gctcgcagtg acaaaaccga acaacattaa
421 gtcaatgttc attgcttgtg ctggaaagaa aactgcagat aaagctgtag acttgtccaa
481 ggatggtctg ctaggtgaca ttctacagga tcttaacact gagacacctc aaataactcc
541 accacctgta atgatactga agaagaaaag atccattgga gcttcaccga atcctttctc
601 tgtgcacacc gccacggcag ttccttcagg aaaaattgct tcccctgtct ccagaaagga
661 gcctccatta actcctgttc ctcttaaacg tgctgaattt gctggcgatg atgtacaggt
721 cgagagtaca gaagaagagc aggagtcagg ggcaatggag tttgaagatg gtgactttga
781 tgagcccatg gaagttgaag aggtggacct ggagcctatg gctgccaagg cttgggacaa
841 agagagtgag ccagcagagg aagtgaaaca agaggcggat tctgggaaag ggaccgtgtc
901 ctacttagga agttttctcc cggatgtctc ttgttgggac attgatcaag aaggtgatag
961 cagtttctca gtgcaagaag ttcaagtgga ttccagtcac ctcccattgg taaaaggggc
1021 agatgaggaa caagtattcc acttttattg gttggatgct tatgaggatc agtacaacca
1081 accaggtgtg gtatttctgt ttgggaaagt ttggattgaa tcagccgaga cccatgtgag
1141 ctgttgtgtc atggtgaaaa atatcgagcg aacgctttac ttccttcccc gtgaaatgaa
1201 aattgatcta aatacgggga aagaaacagg aactccaatt tcaatgaagg atgtttatga
1261 ggaatttgat gagaaaatag caacaaaata taaaattatg aagttcaagt ctaagccagt
1321 ggaaaagaac tatgcttttg agatacctga tgttccagaa aaatctgagt acttggaagt
1381 taaatactcg gctgaaatgc cacagcttcc tcaagatttg aaaggagaaa ctttttctca
1441 tgtatttggg accaacacat ctagcctgga actgttcttg atgaacagaa agatcaaagg
1501 accttgttgg cttgaagtaa aaaagtccac agctcttaat cagccagtca gttggtgtaa
1561 agttgaggca atggctttga aaccagacct ggtgaatgta attaaggatg tcagtccacc
1621 accgcttgtc gtgatggctt tcagcatgaa gacaatgcag aatgcaaaga accatcaaaa
1681 tgagattatt gctatggcag ctttggtcca tcacagtttt gcattggata aagcagcccc
1741 aaagcctccc tttcagtcac acttctgtgt tgtgtctaaa ccaaaggact gtatttttcc
1801 atatgctttc aaagaagtca ttgagaaaaa gaatgtgaag gttgaggttg ctgcaacaga
1861 aagaacactg ctaggttttt tccttgcaaa agttcacaaa attgatcctg atatcattgt
1921 gggtcataat atttatgggt ttgaactgga agtactactg cagagaatta atgtgtgcaa
1981 agctcctcac tggtccaaga taggtcgact gaagcgatcc aacatgccaa agcttggggg
2041 ccggagtgga tttggtgaaa gaaatgctac ctgtggtcga atgatctgtg atgtggaaat
2101 ttcagcaaag gaattgattc gttgtaaaag ctaccatctg tctgaacttg ttcagcagat
2161 tctaaaaact gaaagggttg taatcccaat ggaaaatata caaaatatgt acagtgaatc
2221 ttctcaactg ttatacctgt tggaacacac ctggaaagat gccaagttca ttttgcagat
2281 catgtgtgag ctaaatgttc ttccattagc attgcagatc actaacatcg ctgggaacat
2341 tatgtccagg acgctgatgg gtggacgatc cgagcgtaac gagttcttgt tgcttcatgc
2401 attttacgaa aacaactata ttgtgcctga caagcagatt ttcagaaagc ctcagcaaaa
2461 actgggagat gaagatgaag aaattgatgg agataccaat aaatacaaga aaggacgtaa
2521 gaaaggagct tatgctggag gcttggtttt ggaccccaaa gttggttttt atgataagtt
2581 cattttgctt ctggacttca acagtctata tccttccatc attcaggaat ttaacatttg
2641 ttttacaaca gtacaaagag ttgcttcaga ggcacagaaa gttacagagg atggagaaca
2701 agaacagatc cctgagttgc cagatccaag cttagaaatg ggcattttgc ccagagagat
2761 ccggaaactg gtagaacgga gaaaacaagt caaacagcta atgaaacagc aagacttaaa
2821 tccagacctt attcttcagt atgacattcg acagaaggct ttgaagctca cagcgaacag
2881 tatgtatggt tgcctgggat tttcctatag cagattttac gccaaaccac tggctgcctt
2941 ggtgacatac aaaggaaggg agattttgat gcatacgaaa gagatggtac aaaagatgaa
3001 tcttgaagtt atttatggag atacagattc aattatgata aacaccaata gcaccaatct
3061 ggaagaagta tttaagttgg gaaacaaggt aaaaagtgaa gtgaataagt tgtacaaact
3121 gcttgaaata gacattgatg gggttttcaa gtctctgcta ctgctgaaaa aaaagaagta
3181 cgctgctctg gttgttgagc caacgtcgga tgggaattat gtcaccaaac aggagctcaa
3241 aggattagat atagttagaa gagattggtg tgatcttgct aaagacactg gaaactttgt
3301 gattggccag attctttctg atcaaagccg ggacactata gtggaaaaca ttcagaagag
3361 gctgatagaa attggagaaa atgtgctaaa tggcagtgtc ccagtgagcc agtttgaaat
3421 taacaaggca ttgacaaagg atccccagga ttaccctgat aaaaaaagcc tacctcatgt
3481 acatgttgcc ctctggataa attctcaagg aggcagaaag gtgaaagctg gagatactgt
3541 gtcatatgtc atctgtcagg atggatcaaa cctcactgca agtcagaggg cctatgcgcc
3601 tgagcagctg cagaaacagg ataatctaac cattgacacc cagtactacc tggcccagca
3661 gatccaccca gtcgtggctc ggatctgtga accaatagac ggaattgatg ctgtcctcat
3721 tgcaacgtgg ttgggacttg accccaccca atttagagtt catcattatc ataaagatga
3781 agagaatgat gctctacttg gtggcccagc acagctcact gatgaagaga aatacaggga
3841 ctgtgaaaga ttcaaatgtc catgccctac atgtggaact gagaatattt atgataatgt
3901 ctttgatggt tcgggaacag atatggagcc cagcttgtat cgttgcagta acatcgattg
3961 taaggcttca cctctgacct ttacagtaca actgagcaac aaattgatca tggacattag
4021 acgtttcatt aaaaagtact atgatggctg gttgatatgt gaagagccaa cctgtcgcaa
4081 tcgaactcgt caccttcccc ttcaattctc ccgaactggg cctctttgcc cagcctgcat
4141 gaaagctaca cttcaaccag agtattctga caagtccctg tacacccagc tgtgctttta
4201 ccggtacatt tttgatgcgg agtgtgcact ggagaaactt actaccgatc atgagaaaga
4261 taaattgaag aagcaatttt ttacccccaa agttctgcag gactacagaa aactcaagaa
4321 cacagcagag caattcttgt cccgaagtgg ctactccgaa gtgaatctga gcaaactctt
4381 cgctggttgt gccgtgaaat cctaagggaa tcccaggagt aaccaaggag ggggtagttg
4441 aaaaatccca gcttcctctg tgcctccact ctggccctaa atgctcctcc agcatctgtt
4501 tctcccttgg gactgtgtct catgtttgtg tgaatgtaga ccaggaaagg gggctgcaaa
4561 aatgttgagt ctaatgttcg taagcatcat agaaattcct gtcttcatat taagatgtac
4621 tgctttaaaa cacaactcca gagcccctcc ccaagctccc ctccccaagc tcctgaagac
4681 ccggtttctg agggagggaa attgctactt ggattgagag tagctggaat gtaagtgacc
4741 ccaggctttg ctcagggcct ttagcctatg tcccccccac ataaagagag cttctcagag
4801 cctgactgaa gagctgacgt tttgcttttt catatgccaa ttaaacccgg tctaaatcca
4861 aatgcttctc cagccatcca ggagtggctg tccttttcag tcttgtcttt tatataggta
4921 gctgaggggg aagatttaga agccttgcac tcactaaata gattaaacag agcaggcttg
4981 tttgttgaat tgctccaaag tccaacagac acacactgag caggtgtttt acactcacat
5041 tccctttttg ccccttaaat agaaagtgca ggtaaaggtt tatacaacaa gaaagcacat
5101 tgaaaataat ttgatactct aacaatccat taacatgtgt aggggttacg gtgaggatca
5161 tgtgttgtat tcgaaaaacg gggagaggga tgcttaattg gccctcgctt gctatttttt
5221 tctcatttct tcacaatagg accgtctttg gcagcagcaa aatgtatttc agtatggcag
5281 tctttcctct cttacattat tggtaagatt atactaacaa aatgtttccc cttgtacaat
5341 tatgctgtgt ttttaaaaaa cattgacctg tgtgttttta taaaagaaaa agtatgttgt
5401 gccttcttct taagaataaa gttttctaaa ggg
//