LOCUS X04571 4871 bp mRNA linear HUM 07-OCT-2008
DEFINITION Human mRNA for kidney epidermal growth factor (EGF) precursor.
ACCESSION X04571
VERSION X04571.1
KEYWORDS epidermal growth factor; glycoprotein; growth factor; membrane
protein; signal peptide.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4871)
AUTHORS Bell G.I., Fong N.M., Stempien M.M., Wormsted M.A., Caput D.,
Ku L., Urdea M.S., Rall L.B., Sanchez-Pescador R.
TITLE Human epidermal growth factor precursor: cDNA sequence, expression
in vitro and gene organization
JOURNAL Nucleic Acids Res. 14(21), 8427-8446(1986).
PUBMED 3491360
REFERENCE 2 (bases 4242 to 4243)
AUTHORS Bell G.I.
JOURNAL Submitted (21-MAY-1987) to the INSDC.
FEATURES Location/Qualifiers
source 1..4871
/db_xref="H-InvDB:HIT000321049"
/organism="Homo sapiens"
/mol_type="mRNA"
/dev_stage="adult"
/tissue_type="kidney"
/db_xref="taxon:9606"
CDS 437..4060
/note="precursor polypeptide (AA -22 to 1185)"
/db_xref="GOA:P01133"
/db_xref="H-InvDB:HIT000321049.15"
/db_xref="HGNC:HGNC:3229"
/db_xref="InterPro:IPR000033"
/db_xref="InterPro:IPR000152"
/db_xref="InterPro:IPR000742"
/db_xref="InterPro:IPR001881"
/db_xref="InterPro:IPR009030"
/db_xref="InterPro:IPR011042"
/db_xref="InterPro:IPR013032"
/db_xref="InterPro:IPR016317"
/db_xref="InterPro:IPR018097"
/db_xref="PDB:1IVO"
/db_xref="PDB:1JL9"
/db_xref="PDB:1NQL"
/db_xref="PDB:1P9J"
/db_xref="PDB:2KV4"
/db_xref="PDB:3NJP"
/db_xref="UniProtKB/Swiss-Prot:P01133"
/protein_id="CAA28240.1"
/translation="MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGP
APFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVF
LNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYP
ANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQ
YNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHT
GKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDL
QSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDG
KRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQL
CVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLS
QQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIG
RRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIE
SSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQN
DVGHPFAVAVFEDYVWFSDWAMPSVIRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKP
GADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKN
QVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQ
CLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDID
ECQLGVHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNS
DSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQ
QKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTED
GMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGC
WIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQME
LTQ"
misc_feature 437..3532
/note="put. extracellular domain (AA 1 to 1010)"
sig_peptide 437..502
/note="pot. signal peptide (AA -22 to -1)"
misc_feature 548..556
/note="pot. N-glycosylation site"
misc_feature 746..754
/note="pot. N-glycosylation site"
misc_feature 785..793
/note="pot. N-glycosylation site"
misc_feature 878..886
/note="pot. N-glycosylation site"
misc_feature 1406..1414
/note="pot. N-glycosylation site"
misc_feature 1646..1654
/note="pot. N-glycosylation site"
misc_feature 2222..2230
/note="pot. N-glycosylation site"
misc_feature 2879..2887
/note="pot. N-glycosylation site"
misc_feature 3212..3220
/note="pot. N-glycosylation site"
mat_peptide 3347..3505
/note="mature EGF (AA 949-1001)"
misc_feature 3533..3607
/note="transmembrane domain (AA 1011-1035)"
misc_feature 3608..4057
/note="cytoplasmic domain (AA 1036-1185)"
old_sequence 4242..4243
/note="gc was cg in [1]"
/citation=[1]
misc_feature 4852..4857
/note="pot. polyA signal"
BASE COUNT 1356 a 974 c 1187 g 1354 t
ORIGIN
1 gggagaggaa tcgtatctcc atatttcttc tttcagcccc aatccaaggg ttgtagctgg
61 aactttccat cagttcttcc tttctttttc ctctctaagc ctttgccttg ctctgtcaca
121 gtgaagtcag ccagagcagg gctgttaaac tctgtgaaat ttgtcataag ggtgtcaggt
181 atttcttact ggcttccaaa gaaacataga taaagaaatc tttcctgtgg cttcccttgg
241 caggctgcat tcagaaggtc tctcagttga agaaagagct tggaggacaa cagcacaaca
301 ggagagtaaa agatgcccca gggctgaggc ctccgctcag gcagccgcat ctggggtcaa
361 tcatactcac cttgcccggg ccatgctcca gcaaaatcaa gctgttttct tttgaaagtt
421 caaactcatc aagattatgc tgctcactct tatcattctg ttgccagtag tttcaaaatt
481 tagttttgtt agtctctcag caccgcagca ctggagctgt cctgaaggta ctctcgcagg
541 aaatgggaat tctacttgtg tgggtcctgc acccttctta attttctccc atggaaatag
601 tatctttagg attgacacag aaggaaccaa ttatgagcaa ttggtggtgg atgctggtgt
661 ctcagtgatc atggattttc attataatga gaaaagaatc tattgggtgg atttagaaag
721 acaacttttg caaagagttt ttctgaatgg gtcaaggcaa gagagagtat gtaatataga
781 gaaaaatgtt tctggaatgg caataaattg gataaatgaa gaagttattt ggtcaaatca
841 acaggaagga atcattacag taacagatat gaaaggaaat aattcccaca ttcttttaag
901 tgctttaaaa tatcctgcaa atgtagcagt tgatccagta gaaaggttta tattttggtc
961 ttcagaggtg gctggaagcc tttatagagc agatctcgat ggtgtgggag tgaaggctct
1021 gttggagaca tcagagaaaa taacagctgt gtcattggat gtgcttgata agcggctgtt
1081 ttggattcag tacaacagag aaggaagcaa ttctcttatt tgctcctgtg attatgatgg
1141 aggttctgtc cacattagta aacatccaac acagcataat ttgtttgcaa tgtccctttt
1201 tggtgaccgt atcttctatt caacatggaa aatgaagaca atttggatag ccaacaaaca
1261 cactggaaag gacatggtta gaattaacct ccattcatca tttgtaccac ttggtgaact
1321 gaaagtagtg catccacttg cacaacccaa ggcagaagat gacacttggg agcctgagca
1381 gaaactttgc aaattgagga aaggaaactg cagcagcact gtgtgtgggc aagacctcca
1441 gtcacacttg tgcatgtgtg cagagggata cgccctaagt cgagaccgga agtactgtga
1501 agatgttaat gaatgtgctt tttggaatca tggctgtact cttgggtgta aaaacacccc
1561 tggatcctat tactgcacgt gccctgtagg atttgttctg cttcctgatg ggaaacgatg
1621 tcatcaactt gtttcctgtc cacgcaatgt gtctgaatgc agccatgact gtgttctgac
1681 atcagaaggt cccttatgtt tctgtcctga aggctcagtg cttgagagag atgggaaaac
1741 atgtagcggt tgttcctcac ccgataatgg tggatgtagc cagctctgcg ttcctcttag
1801 cccagtatcc tgggaatgtg attgctttcc tgggtatgac ctacaactgg atgaaaaaag
1861 ctgtgcagct tcaggaccac aaccattttt gctgtttgcc aattctcaag atattcgaca
1921 catgcatttt gatggaacag actatggaac tctgctcagc cagcagatgg gaatggttta
1981 tgccctagat catgaccctg tggaaaataa gatatacttt gcccatacag ccctgaagtg
2041 gatagagaga gctaatatgg atggttccca gcgagaaagg cttattgagg aaggagtaga
2101 tgtgccagaa ggtcttgctg tggactggat tggccgtaga ttctattgga cagacagagg
2161 gaaatctctg attggaagga gtgatttaaa tgggaaacgt tccaaaataa tcactaagga
2221 gaacatctct caaccacgag gaattgctgt tcatccaatg gccaagagat tattctggac
2281 tgatacaggg attaatccac gaattgaaag ttcttccctc caaggccttg gccgtctggt
2341 tatagccagc tctgatctaa tctggcccag tggaataacg attgacttct taactgacaa
2401 gttgtactgg tgcgatgcca agcagtctgt gattgaaatg gccaatctgg atggttcaaa
2461 acgccgaaga cttacccaga atgatgtagg tcacccattt gctgtagcag tgtttgagga
2521 ttatgtgtgg ttctcagatt gggctatgcc atcagtaata agagtaaaca agaggactgg
2581 caaagataga gtacgtctcc aaggcagcat gctgaagccc tcatcactgg ttgtggttca
2641 tccattggca aaaccaggag cagatccctg cttatatcaa aacggaggct gtgaacatat
2701 ttgcaaaaag aggcttggaa ctgcttggtg ttcgtgtcgt gaaggtttta tgaaagcctc
2761 agatgggaaa acgtgtctgg ctctggatgg tcatcagctg ttggcaggtg gtgaagttga
2821 tctaaagaac caagtaacac cattggacat cttgtccaag actagagtgt cagaagataa
2881 cattacagaa tctcaacaca tgctagtggc tgaaatcatg gtgtcagatc aagatgactg
2941 tgctcctgtg ggatgcagca tgtatgctcg gtgtatttca gagggagagg atgccacatg
3001 tcagtgtttg aaaggatttg ctggggatgg aaaactatgt tctgatatag atgaatgtga
3061 gatgggtgtc ccagtgtgcc cccctgcctc ctccaagtgc atcaacaccg aaggtggtta
3121 tgtctgccgg tgctcagaag gctaccaagg agatgggatt cactgtcttg atattgatga
3181 gtgccaactg ggggtgcaca gctgtggaga gaatgccagc tgcacaaata cagagggagg
3241 ctatacctgc atgtgtgctg gacgcctgtc tgaaccagga ctgatttgcc ctgactctac
3301 tccaccccct cacctcaggg aagatgacca ccactattcc gtaagaaata gtgactctga
3361 atgtcccctg tcccacgatg ggtactgcct ccatgatggt gtgtgcatgt atattgaagc
3421 attggacaag tatgcatgca actgtgttgt tggctacatc ggggagcgat gtcagtaccg
3481 agacctgaag tggtgggaac tgcgccacgc tggccacggg cagcagcaga aggtcatcgt
3541 ggtggctgtc tgcgtggtgg tgcttgtcat gctgctcctc ctgagcctgt ggggggccca
3601 ctactacagg actcagaagc tgctatcgaa aaacccaaag aatccttatg aggagtcgag
3661 cagagatgtg aggagtcgca ggcctgctga cactgaggat gggatgtcct cttgccctca
3721 accttggttt gtggttataa aagaacacca agacctcaag aatgggggtc aaccagtggc
3781 tggtgaggat ggccaggcag cagatgggtc aatgcaacca acttcatgga ggcaggagcc
3841 ccagttatgt ggaatgggca cagagcaagg ctgctggatt ccagtatcca gtgataaggg
3901 ctcctgtccc caggtaatgg agcgaagctt tcatatgccc tcctatggga cacagaccct
3961 tgaagggggt gtcgagaagc cccattctct cctatcagct aacccattat ggcaacaaag
4021 ggccctggac ccaccacacc aaatggagct gactcagtga aaactggaat taaaaggaaa
4081 gtcaagaaga atgaactatg tcgatgcaca gtatcttttc tttcaaaagt agagcaaaac
4141 tataggtttt ggttccacaa tctctacgac taatcaccta ctcaatgcct ggagacagat
4201 acgtagttgt gcttttgttt gctcttttaa gcagtctcac tgcagtctta tttccaagta
4261 agagtactgg gagaatcact aggtaactta ttagaaaccc aaattgggac aacagtgctt
4321 tgtaaattgt gttgtcttca gcagtcaata caaatagatt tttgtttttg ttgttcctgc
4381 agccccagaa gaaattaggg gttaaagcag acagtcacac tggtttggtc agttacaaag
4441 taatttcttt gatctggaca gaacatttat atcagtttca tgaaatgatt ggaatattac
4501 aataccgtta agatacagtg taggcattta actcctcatt ggcgtggtcc atgctgatga
4561 ttttgccaaa atgagttgtg atgaatcaat gaaaaatgta atttagaaac tgatttcttc
4621 agaattagat ggccttattt tttaaaatat ttgaatgaaa acattttatt tttaaaatat
4681 tacacaggag gccttcggag tttcttagtc attactgtcc ttttccccta cagaattttc
4741 cctcttggtg tgattgcaca gaatttgtat gtattttcag ttacaagatt gtaagtaaat
4801 tgcctgattt gttttcatta tagacaacga tgaatttctt ctaattattt aaataaaatc
4861 accaaaaaca t
//