LOCUS X04366 3007 bp mRNA linear HUM 18-APR-2005
DEFINITION Human mRNA for calcium activated neutral protease large subunit
(muCANP, calpain, EC 3.4.22.17).
ACCESSION X04366
VERSION X04366.1
KEYWORDS calcium dependant protein; calpain; neutral protease; protease.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3007)
AUTHORS Aoki K., Imajoh S., Ohno S., Emori Y., Koike M., Kosaki G.,
Suzuki K.
TITLE Complete amino acid sequence of the large subunit of the
low-Ca2+-requiring form of human Ca2+-activated neutral protease
(muCANP) deduced from its cDNA sequence
JOURNAL FEBS Lett. 205(2), 313-317(1986).
PUBMED 3017764
REFERENCE 2 (bases 1 to 3007)
AUTHORS Zhang W., Lane R.D., Mellgren R.L.
TITLE The major calpain isozymes are long-lived proteins. Design of an
antisense strategy for calpain depletion in cultured cells
JOURNAL J Biol Chem 271(31), 18825-18830(1996).
PUBMED 8702541
COMMENT In domain IV (Ca(2+)-binding domain) four consecutive EF-hand
sequences are found.
Data kindly reviewed (10-DEC-1986) by K. Suzuki
FEATURES Location/Qualifiers
source 1..3007
/db_xref="H-InvDB:HIT000321029"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 144..2288
/note="CANP, large subunit (aa 1-714)"
/db_xref="GOA:P07384"
/db_xref="H-InvDB:HIT000321029.14"
/db_xref="HGNC:HGNC:1476"
/db_xref="InterPro:IPR000169"
/db_xref="InterPro:IPR001300"
/db_xref="InterPro:IPR002048"
/db_xref="InterPro:IPR011992"
/db_xref="InterPro:IPR018247"
/db_xref="InterPro:IPR022682"
/db_xref="InterPro:IPR022683"
/db_xref="InterPro:IPR022684"
/db_xref="InterPro:IPR029643"
/db_xref="InterPro:IPR033883"
/db_xref="InterPro:IPR036213"
/db_xref="InterPro:IPR038765"
/db_xref="PDB:1ZCM"
/db_xref="PDB:2ARY"
/db_xref="UniProtKB/Swiss-Prot:P07384"
/protein_id="CAA27881.1"
/translation="MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQ
LRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGA
TRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWV
DVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFT
GGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSV
TGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEF
WMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFW
VNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPP
ELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGD
FVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKEL
RTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIF
RKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRL
ETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA"
misc_feature 404..405
/note="boundary of domains I/II"
misc_feature 1124..1125
/note="boundary of domains II/III"
misc_feature 1850..1851
/note="boundary of domains III/IV"
misc_feature 2982..2988
/note="pot. polyA signal"
polyA_site 3007..3007
/note="polyA site"
BASE COUNT 592 a 917 c 918 g 580 t
ORIGIN
1 aaggagagag ggagggcgga gggcggaggg gcggcgggag gagggcgggg aggagcgctc
61 ttcctggttg ggccctgccc tgagctgcca ccgggaagcc agcctcaggg actgcagcga
121 cccccaaaca cccctccccc aggatgtcgg aggagatcat cacgccggtg tactgcactg
181 gggtgtcagc ccaagtgcag aagcagcggg ccagggagct gggcctgggc cgccatgaga
241 atgccatcaa gtacctgggc caggattatg agcagctgcg ggtgcgatgc ctgcagagtg
301 ggaccctctt ccgtgatgag gccttccccc cggtacccca gagcctgggt tacaaggacc
361 tgggtcccaa ttcctccaag acctatggca tcaagtggaa gcgtcccacg gaactgctgt
421 caaaccccca gttcattgtg gatggagcta cccgcacaga catctgccag ggagcactgg
481 gggactgctg gctcttggcg gccattgcct ccctcactct caacgacacc ctcctgcacc
541 gagtggttcc gcacggccag agcttccaga atggctatgc cggcatcttc catttccagc
601 tgtggcaatt tggggagtgg gtggacgtgg tcgtggatga cctgctgccc atcaaggacg
661 ggaagctagt gttcgtgcac tctgccgaag gcaacgagtt ctggagcgcc ctgcttgaga
721 aggcctatgc caaggtaaat ggcagctacg aggccctgtc agggggcagc acctcagagg
781 gctttgagga cttcacaggc ggggttaccg agtggtacga gttgcgcaag gctcccagtg
841 acctctacca gatcatcctc aaggcgctgg agcggggctc cctgctgggc tgctccatag
901 acatctccag cgttctagac atggaggcca tcactttcaa gaagttggtg aagggccatg
961 cctactctgt gaccggggcc aagcaggtga actaccgagg ccaggtggtg agcctgatcc
1021 ggatgcggaa cccctggggc gaggtggagt ggacgggagc ctggagcgac agctcctcag
1081 agtggaacaa cgtggaccca tatgaacggg accagctccg ggtcaagatg gaggacgggg
1141 agttctggat gtcattccga gacttcatgc gggagttcac ccgcctggag atctgcaacc
1201 tcacacccga cgccctcaag agccggacca tccgcaaatg gaacaccaca ctctacgaag
1261 gcacctggcg gcgggggagc accgcggggg gctgccgaaa ctacccagcc accttctggg
1321 tgaaccctca gttcaagatc cggctggatg agacggatga cccggacgac tacggggacc
1381 gcgagtcagg ctgcagcttc gtgctcgccc ttatgcagaa gcaccgtcgc cgcgagcgcc
1441 gcttcggccg cgacatggag actattggct tcgcggtcta cgaggtccct ccggagctgg
1501 tgggccagcc ggccgtacac ttgaagcgtg acttcttcct ggccaatgcg tctcgggcgc
1561 gctcagagca gttcatcaac ctgcgagagg tcagcacccg cttccgcctg ccacccgggg
1621 agtatgtggt ggtgccctcc accttcgagc ccaacaagga gggcgacttc gtgctgcgct
1681 tcttctcaga gaagagtgct gggactgtgg agctggatga ccagatccag gccaatctcc
1741 ccgatgagca agtgctctca gaagaggaga ttgacgagaa cttcaaggcc ctcttcaggc
1801 agctggcagg ggaggacatg gagatcagcg tgaaggagtt gcggacaatc ctcaatagga
1861 tcatcagcaa acacaaagac ctgcggacca agggcttcag cctagagtcg tgccgcagca
1921 tggtgaacct catggatcgt gatggcaatg ggaagctggg cctggtggag ttcaacatcc
1981 tgtggaaccg catccggaat tacctgtcca tcttccggaa gtttgacctg gacaagtcgg
2041 gcagcatgag tgcctacgag atgcggatgg ccattgagtc ggcaggcttc aagctcaaca
2101 agaagctgta cgagctcatc atcacccgct actcggagcc cgacctggcg gtcgactttg
2161 acaatttcgt ttgctgcctg gtgcggctag agaccatgtt ccgatttttc aaaactctgg
2221 acacagatct ggatggagtt gtgacctttg acttgtttaa gtggttgcag ctgaccatgt
2281 ttgcatgagg cagggactcg gtcccccttg ccgtgctccc ctccctcctc gtctgccaag
2341 cctcgcctcc taccacacca caccaggcca ccccagctgc aagtgccttc cttggagcag
2401 agaggcagcc tcgtcctcct gtcccctctc ctcccagcca ccatcgttca tctgctccgg
2461 gcagaactgt gtggcccctg cctgtgccag ccatgggctc gggatggact ccctgggccc
2521 cacccattgc caagccagga aggcagcttt cgcttgttcc tgcctcggga cagccccggg
2581 tttccccagc atcctgatgt gtcccctctc cccacttcag aggccaccca ctcagcacca
2641 ccggcctggc cttgcctgca gactataaac tataaccact agctcgacac agtctgcagt
2701 ccaggcgtgt ggagccgcct cccggctcgg ggaggccccg gggctgggaa cgcctgtgcc
2761 ttcctgcgcc gaagccaacg ccccctctgt ccttccctgg ccctgctgcc gaccaggagc
2821 tgcccagcct gtgggcggtc ggccttccct ccttcgctcc ttttttatat tagtgatttt
2881 aaaggggact cttcagggac ttgtgtactg gttatggggg tgccagaggc actaggcttg
2941 gggtggggag gtcccgtgtt ccatatagag gaaccccaaa taataaaagg ccccacatct
3001 gtctgtg
//