LOCUS HSU70136 5041 bp mRNA linear HUM 29-SEP-1996
DEFINITION Human megakaryocyte stimulating factor mRNA, complete cds.
ACCESSION U70136
VERSION U70136.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5041)
AUTHORS Turner,K.J., Fitz,L.J., Temple,P., Jacobs,K., Larson,D.,
Leary,A.C., Kelleher,K., Giannotti,J., Calvetti,J., Fitzgerald,M.,
Kriz,M.-J., Ferenz,C., Grobholz,J., Fraser,H., Bean,K.,
Norton,C.R., Gesner,T., Bhatia,S., Kriz,R., Hewick,R. and
Clark,S.C.
TITLE Purification, Biochemical Characterization, and Cloning of a Novel
Megakaryocyte Stimulating Factor that has Megakaryocyte Colony
Stimulating Activity
JOURNAL Blood 78 (Suppl. 1), 279 (1991)
REFERENCE 2 (bases 1 to 5041)
AUTHORS Merberg,D.M., Fitz,L.J., Temple,P., Giannotti,J., Murtha,P.,
Fitzgerald,M., Scaltreto,J., Kelleher,K., Preissner,K., Kriz,R.,
Jacobs,K. and Turner,K.
TITLE A Comparison of Vitronectin and Megakaryocyte Stimulating Factor
JOURNAL (in) Preissner,K.T., Rosenblatt,S., Kost,C., Wegerhoff,J. and
Mosher,D.F. (Eds.);
BIOLOGY OF VITRONECTINS AND THEIR RECEPTORS.: 45-52;
Elsevier Science Publishers B.V. (1993)
REFERENCE 3 (bases 1 to 5041)
AUTHORS Turner,K.J., Fitz,L.J., Temple,P., Jacobs,K., Larson,D.,
Leary,A.C., Kelleher,K., Giannotti,J., Calvetti,J., Fitzgerald,M.,
Kriz,M.-J., Ferenz,C., Grobholz,J., Fraser,H., Bean,K.,
Norton,C.R., Gesner,T., Bhatia,S., Kriz,R., Hewick,R. and
Clark,S.C.
TITLE Direct Submission
JOURNAL Submitted (09-SEP-1996) Genetics Institute, 87 CambridgePark Drive,
Cambridge, MA 02140, USA
FEATURES Location/Qualifiers
source 1..5041
/db_xref="H-InvDB:HIT000221094"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_type="PHA and PMA activated human peripheral blood
mononuclear cells"
CDS 34..4248
/note="MSF"
/codon_start=1
/product="megakaryocyte stimulating factor"
/protein_id="AAB09089.1"
/translation="MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATC
NCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYES
FCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSV
SENQESSSSSSSSSSSSTIWKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPV
VDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSL
TVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPK
AETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTT
PKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKE
PAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAP
TTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPK
EPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTAPKEPAPTTPKETAPTTPKKL
TPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAP
TTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKE
PTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPA
PKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSE
VSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTT
AKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFK
ITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKK
PTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLV
EVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNIC
NGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNC
EGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFF
KRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGEMTQVRRRRFERAIGPSQTHTIRIQY
SPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNI
DVPSRTARAITTRSGQTLSKVWYNCP"
BASE COUNT 1710 a 1516 c 806 g 1009 t
ORIGIN
1 gcggccgcga ctattcggta cctgaaaaca acgatggcat ggaaaacact tcccatttac
61 ctgttgttgc tgctgtctgt tttcgtgatt cagcaagttt catctcaaga tttatcaagc
121 tgtgcaggga gatgtgggga agggtattct agagatgcca cctgcaactg tgattataac
181 tgtcaacact acatggagtg ctgccctgat ttcaagagag tctgcactgc ggagctttcc
241 tgtaaaggcc gctgctttga gtccttcgag agagggaggg agtgtgactg cgacgcccaa
301 tgtaagaagt atgacaagtg ctgtcccgat tatgagagtt tctgtgcaga agtgcataat
361 cccacatcac caccatcttc aaagaaagca cctccacctt caggagcatc tcaaaccatc
421 aaatcaacaa ccaaacgttc acccaaacca ccaaacaaga agaagactaa gaaagttata
481 gaatcagagg aaataacaga agaacattct gtttctgaaa atcaagagtc ctcctcctcc
541 tcctcctctt cctcttcttc ttcaacaatt tggaaaatca agtcttccaa aaattcagct
601 gctaatagag aattacagaa gaaactcaaa gtaaaagata acaagaagaa cagaactaaa
661 aagaaaccta cccccaaacc accagttgta gatgaagctg gaagtggatt ggacaatggt
721 gacttcaagg tcacaactcc tgacacgtct accacccaac acaataaagt cagcacatct
781 cccaagatca caacagcaaa accaataaat cccagaccca gtcttccacc taattctgat
841 acatctaaag agacgtcttt gacagtgaat aaagagacaa cagttgaaac taaagaaact
901 actacaacaa ataaacagac ttcaactgat ggaaaagaga agactacttc cgctaaagag
961 acacaaagta tagagaaaac atctgctaaa gatttagcac ccacatctaa agtgctggct
1021 aaacctacac ccaaagctga aactacaacc aaaggccctg ctctcaccac tcccaaggag
1081 cccacgccca ccactcccaa ggagcctgca tctaccacac ccaaagagcc cacacctacc
1141 accatcaagt ctgcacccac cacccccaag gagcctgcac ccaccaccac caagtctgca
1201 cccaccactc ccaaggagcc tgcacccacc accaccaagg agcctgcacc caccactccc
1261 aaggagcctg cacccaccac caccaaggag cctgcaccca ccaccaccaa gtctgcaccc
1321 accactccca aggagcctgc acccaccacc cccaagaagc ctgccccaac tacccccaag
1381 gagcctgcac ccaccactcc caaggagcct acacccacca ctcccaagga gcctgcaccc
1441 accaccaagg agcctgcacc caccactccc aaagagcctg cacccactgc ccccaagaag
1501 cctgccccaa ctacccccaa ggagcctgca cccaccactc ccaaggagcc tgcacccacc
1561 accaccaagg agccttcacc caccactccc aaggagcctg cacccaccac caccaagtct
1621 gcacccacca ctaccaagga gcctgcaccc accactacca agtctgcacc caccactccc
1681 aaggagcctt cacccaccac caccaaggag cctgcaccca ccactcccaa ggagcctgca
1741 cccaccaccc ccaagaagcc tgccccaact acccccaagg agcctgcacc caccactccc
1801 aaggaacctg cacccaccac caccaagaag cctgcaccca ccgctcccaa agagcctgcc
1861 ccaactaccc ccaaggagac tgcacccacc acccccaaga agctcacgcc caccaccccc
1921 gagaagctcg cacccaccac ccctgagaag cccgcaccca ccacccctga ggagctcgca
1981 cccaccaccc ctgaggagcc cacacccacc acccctgagg agcctgctcc caccactccc
2041 aaggcagcgg ctcccaacac ccctaaggag cctgctccaa ctacccctaa ggagcctgct
2101 ccaactaccc ctaaggagcc tgctccaact acccctaagg agactgctcc aactacccct
2161 aaagggactg ctccaactac cctcaaggaa cctgcaccca ctactcccaa gaagcctgcc
2221 cccaaggagc ttgcacccac caccaccaag gagcccacat ccaccacctc tgacaagccc
2281 gctccaacta cccctaaggg gactgctcca actaccccta aggagcctgc tccaactacc
2341 cctaaggagc ctgctccaac tacccctaag gggactgctc caactaccct caaggaacct
2401 gcacccacta ctcccaagaa gcctgccccc aaggagcttg cacccaccac caccaagggg
2461 cccacatcca ccacctctga caagcctgct ccaactacac ctaaggagac tgctccaact
2521 acccccaagg agcctgcacc cactaccccc aagaagcctg ctccaactac tcctgagaca
2581 cctcctccaa ccacttcaga ggtctctact ccaactacca ccaaggagcc taccactatc
2641 cacaaaagcc ctgatgaatc aactcctgag ctttctgcag aacccacacc aaaagctctt
2701 gaaaacagtc ccaaggaacc tggtgtacct acaactaaga ctcctgcagc gactaaacct
2761 gaaatgacta caacagctaa agacaagaca acagaaagag acttacgtac tacacctgaa
2821 actacaactg ctgcacctaa gatgacaaaa gagacagcaa ctacaacaga aaaaactacc
2881 gaatccaaaa taacagctac aaccacacaa gtaacatcta ccacaactca agataccaca
2941 ccattcaaaa ttactactct taaaacaact actcttgcac ccaaagtaac tacaacaaaa
3001 aagacaatta ctaccactga gattatgaac aaacctgaag aaacagctaa accaaaagac
3061 agagctacta attctaaagc gacaactcct aaacctcaaa agccaaccaa agcacccaaa
3121 aaacccactt ctaccaaaaa gccaaaaaca atgcctagag tgagaaaacc aaagacgaca
3181 ccaactcccc gcaagatgac atcaacaatg ccagaattga accctacctc aagaatagca
3241 gaagccatgc tccaaaccac caccagacct aaccaaactc caaactccaa actagttgaa
3301 gtaaatccaa agagtgaaga tgcaggtggt gctgaaggag aaacacctca tatgcttctc
3361 aggccccatg tgttcatgcc tgaagttact cccgacatgg attacttacc gagagtaccc
3421 aatcaaggca ttatcatcaa tcccatgctt tccgatgaga ccaatatatg caatggtaag
3481 ccagtagatg gactgactac tttgcgcaat gggacattag ttgcattccg aggtcattat
3541 ttctggatgc taagtccatt cagtccacca tctccagctc gcagaattac tgaagtttgg
3601 ggtattcctt cccccattga tactgttttt actaggtgca actgtgaagg aaaaactttc
3661 ttctttaagg attctcagta ctggcgtttt accaatgata taaaagatgc agggtacccc
3721 aaaccaattt tcaaaggatt tggaggacta actggacaaa tagtggcagc gctttcaaca
3781 gctaaatata agaactggcc tgaatctgtg tattttttca agagaggtgg cagcattcag
3841 cagtatattt ataaacagga acctgtacag aagtgccctg gaagaaggcc tgctctaaat
3901 tatccagtgt atggagaaat gacacaggtt aggagacgtc gctttgaacg tgctatagga
3961 ccttctcaaa cacacaccat cagaattcaa tattcacctg ccagactggc ttatcaagac
4021 aaaggtgtcc ttcataatga agttaaagtg agtatactgt ggagaggact tccaaatgtg
4081 gttacctcag ctatatcact gcccaacatc agaaaacctg acggctatga ttactatgcc
4141 ttttctaaag atcaatacta taacattgat gtgcctagta gaacagcaag agcaattact
4201 actcgttctg ggcagacctt atccaaagtc tggtacaact gtccttagac tgatgagcaa
4261 aggaggagtc aactaatgaa gaaatgaata ataaattttg acactgaaaa acattttatt
4321 aataaagaat attgacatga gtataccagt ttatatataa aaatgttttt aaacttgaca
4381 atcattacac taaaacagat ttgataatct tattcacagt tgttattgtt tacagaccat
4441 ttaattaata tttcctctgt ttattcctcc tctccctccc attgcatggc tcacacctgt
4501 aaaagaaaaa agaatcaaat tgaatatatc ttttaagaat tcaaaactag tgtattcact
4561 taccctagtt cattataaaa aatatctagg cattgtggat ataaaactgt tgggtattct
4621 acaacttcaa tggaaattat tacaagcaga ttaatccctc tttttgtgac acaagtacaa
4681 tctaaaagtt atattggaaa acatggaaat attaaaattt tacactttta ctagctaaaa
4741 cataatcaca aagctttatc gtgttgtata aaaaaattaa caatataatg gcaataggta
4801 gagatacaac aaatgaatat aacactataa cacttcatat tttccaaatc ttaatttgga
4861 tttaaggaag aaatcaataa atataaaata taagcacata tttattatat atctaaggta
4921 tacaaatctg tctacatgaa gtttacagat tggtaaatat cacctgctca acatgtaatt
4981 atttaataaa actttggaac attaaaaaaa taaattggag gcttaaaaaa aaaaaaaaaa
5041 a
//