LOCUS HSU69263 3496 bp mRNA linear HUM 16-JAN-2001
DEFINITION Homo sapiens matrilin-2 precursor, mRNA, complete cds.
ACCESSION U69263
VERSION U69263.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 2055 to 3008)
AUTHORS Deak,F., Piecha,D., Bachrati,C., Paulsson,M. and Kiss,I.
TITLE Primary structure and expression of matrilin-2, the closest
relative of cartilage matrix protein within the von Willebrand
factor type A-like module superfamily
JOURNAL J. Biol. Chem. 272 (14), 9268-9274 (1997)
PUBMED 9083061
REFERENCE 2 (bases 1 to 3496)
AUTHORS Muratoglu,S., Krysan,K., Balazs,M., Sheng,H., Zakany,R., Modis,L.,
Kiss,I. and Deak,F.
TITLE Primary structure of human matrilin-2, chromosome location of the
MATN2 gene and conservation of an AT-AC intron in matrilin genes
JOURNAL Cytogenet. Cell Genet. 90 (3-4), 323-327 (2000)
PUBMED 11124542
REFERENCE 3 (bases 2075 to 2394)
AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
Wilson,R.
TITLE The WashU-Merck EST Project
JOURNAL Unpublished
REFERENCE 4 (bases 2055 to 3008)
AUTHORS Deak,F.
TITLE Direct Submission
JOURNAL Submitted (04-SEP-1996) Institute of Biochemistry, Biological
Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62,
Szeged H-6701, Hungary
REFERENCE 5 (bases 1 to 3496)
AUTHORS Deak,F.
TITLE Direct Submission
JOURNAL Submitted (08-NOV-2000) Institute of Biochemistry, Biological
Research Centre, Hungarian Academy of Sciences, Temesvari krt. 62,
Szeged H-6701, Hungary
REMARK Sequence update by submitter
COMMENT On Nov 8, 2000 this sequence version replaced U69263.1.
FEATURES Location/Qualifiers
source 1..3496
/db_xref="H-InvDB:HIT000221069"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="8"
/map="8q22"
/clone="323380"
/cell_type="senescent fibroblast; Hep-2 keratin-positive
epithelial cell line"
/clone_lib="Soares senescent fibroblast NbHSF"
5'UTR 1..125
CDS 126..2996
/codon_start=1
/product="matrilin-2 precursor"
/protein_id="AAC51260.2"
/translation="MEKMLAGCFLLILGQIVLLPAEARERSRGRSISRGRHARTHPQT
ALLESSCENKRADLVFIIDSSRSVNTHDYAKVKEFIVDILQFLDIGPDVTRVGLLQYG
STVKNEFSLKTFKRKSEVERAVKRMRHLSTGTMTGLAIQYALNIAFSEAEGARPLREN
VPRVIMIVTDGRPQDSVAEVAAKARDTGILIFAIGVGQVDFNTLKSIGSEPHEDHVFL
VANFSQIETLTSVFQKKLCTAHMCSTLEHNCAHFCINIPGSYVCRCKQGYILNSDQTT
CRIQDLCAMEDHNCEQLCVNVPGSFVCQCYSGYALAEDGKRCVAVDYCASENHGCEHE
CVNADGSYLCQCHEGFALNPDKKTCTKIDYCASSNHGCQHECVNTDDSYSCHCLKGFT
LNPDKKTCRRINYCALNKPGCEHECVNMEESYYCRCHRGYTLDPNGKTCSRVDHCAQQ
DHGCEQLCLNTEDSFVCQCSEGFLINEDLKTCSRVDYCLLSDHGCEYSCVNMDRSFAC
QCPEGHVLRSDGKTCAKLDSCALGDHGCEHSCVSSEDSFVCQCFEGYILREDGKTCRR
KDVCQAIDHGCEHICVNSDDSYTCECLVGFRLAEDGKRCRRKDVCKSTHHGCEHICVN
NGNSYICKCSEGFVLAEDGRRCKKCTEGPIDLVFVIDGSKSLGEENFEVVKQFVTGII
DSLTISPKAARVGLLQYSTQVHTEFTLRNFNSAKDMKKAVAHMKYMGKGSMTGLALKH
MFERSFTQGEGARPFSTRVPRAAIVFTDGRAQDDVSEWASKAKANGITMYAVGVGKAI
EEELQEIASEPTNKHLFYAEDFSTMDEISEKLKKGICEALEDSDGRQDSPAGELPKTV
QQPTESEPVTINIQDLLSCSNFAVQHRYLFEEDNLLRSTQKLSHSTKPSGSPLEEKHD
QCKCENLIMFQNLANEEVRKLTQRLEEMTQRMEALENRLRYR"
mat_peptide 195..2993
/product="matrilin-2"
misc_feature 267..836
/note="encodes von Willebrand factor type A motif"
/number=1
misc_feature 837..959
/note="encodes epidermal growth factor-like motif"
/number=1
misc_feature 960..1082
/note="encodes epidermal growth factor-like motif"
/number=2
misc_feature 1083..1205
/note="encodes epidermal growth factor-like motif"
/number=3
misc_feature 1206..1328
/note="encodes epidermal growth factor-like motif"
/number=4
misc_feature 1329..1451
/note="encodes epidermal growth factor-like motif"
/number=5
misc_feature 1452..1574
/note="encodes epidermal growth factor-like motif"
/number=6
misc_feature 1575..1697
/note="encodes epidermal growth factor-like motif"
/number=7
misc_feature 1698..1820
/note="encodes epidermal growth factor-like motif"
/number=8
misc_feature 1821..1943
/note="encodes epidermal growth factor-like motif"
/number=9
misc_feature 1944..2066
/note="encodes epidermal growth factor-like motif"
/number=10
misc_feature 2067..2633
/note="encodes von Willebrand factor type A motif"
/number=2
misc_feature 2880..2993
/note="encodes putative coiled-coil"
3'UTR 2997..3496
regulatory 3475..3480
/regulatory_class="polyA_signal_sequence"
BASE COUNT 963 a 826 c 889 g 818 t
ORIGIN
1 cgaactctga aaaggcgggg cagcgggcct gcagctcctg gagttcaggg agacccggaa
61 atctcaccct gccctcttct tgtgttgtgt ttgtcacagc cttgcccctc ttgctcgcct
121 tgaaaatgga aaagatgctc gcaggctgct ttctgctgat cctcggacag atcgtcctcc
181 tccctgccga ggccagggag cggtcacgtg ggaggtccat ctctaggggc agacacgctc
241 ggacccaccc gcagacggcc cttctggaga gttcctgtga gaacaagcgg gcagacctgg
301 ttttcatcat tgacagctct cgcagtgtca acacccatga ctatgcaaag gtcaaggagt
361 tcatcgtgga catcttgcaa ttcttggaca ttggtcctga tgtcacccga gtgggcctgc
421 tccaatatgg cagcactgtc aagaatgagt tctccctcaa gaccttcaag aggaagtccg
481 aggtggagcg tgctgtcaag aggatgcggc atctgtccac gggcaccatg accgggctgg
541 ccatccagta tgccctgaac atcgcattct cagaagcaga gggggcccgg cccctgaggg
601 agaatgtgcc acgggtcata atgatcgtga cggatgggag acctcaggac tccgtggccg
661 aggtggctgc taaggcacgg gacacgggca tcctaatctt tgccattggt gtgggccagg
721 tagacttcaa caccttgaag tccattggga gtgagcccca tgaggaccat gtcttccttg
781 tggccaattt cagccagatt gagacgctga cctccgtgtt ccagaagaag ttgtgcacgg
841 cccacatgtg cagcaccctg gagcataact gtgcccactt ctgcatcaac atccctggct
901 catacgtctg caggtgcaaa caaggctaca ttctcaactc ggatcagacg acttgcagaa
961 tccaggatct gtgtgccatg gaggaccaca actgtgagca gctctgtgtg aatgtgccgg
1021 gctccttcgt ctgccagtgc tacagtggct acgccctggc tgaggatggg aagaggtgtg
1081 tggctgtgga ctactgtgcc tcagaaaacc acggatgtga acatgagtgt gtaaatgctg
1141 atggctccta cctttgccag tgccatgaag gatttgctct taacccagat aaaaaaacgt
1201 gcacaaagat agactactgt gcctcatcta atcacggatg tcagcacgag tgtgttaaca
1261 cagatgattc ctattcctgc cactgcctga aaggctttac cctgaatcca gataagaaaa
1321 cctgcagaag gatcaactac tgtgcactga acaaaccggg ctgtgagcat gagtgcgtca
1381 acatggagga gagctactac tgccgctgcc accgtggcta cactctggac cccaatggca
1441 aaacctgcag ccgagtggac cactgtgcac agcaggacca tggctgtgag cagctgtgtc
1501 tgaacacgga ggattccttc gtctgccagt gctcagaagg cttcctcatc aacgaggacc
1561 tcaagacctg ctcccgggtg gattactgcc tgctgagtga ccatggttgt gaatactcct
1621 gtgtcaacat ggacagatcc tttgcctgtc agtgtcctga gggacacgtg ctccgcagcg
1681 atgggaagac gtgtgcaaaa ttggactctt gtgctctggg ggaccacggt tgtgaacatt
1741 cgtgtgtaag cagtgaagat tcgtttgtgt gccagtgctt tgaaggttat atactccgtg
1801 aagatggaaa aacctgcaga aggaaagatg tctgccaagc tatagaccat ggctgtgaac
1861 acatttgtgt gaacagtgat gactcataca cgtgcgagtg cttggtggga ttccggctcg
1921 ctgaggatgg gaaacgctgc cgaaggaagg atgtctgcaa atcaacccac catggctgcg
1981 aacacatttg tgttaataat gggaattcct acatctgcaa atgctcagag ggatttgttc
2041 tagctgagga cggaagacgg tgcaagaaat gcactgaagg cccaattgac ctggtctttg
2101 tgatcgatgg atccaagagt cttggagaag agaattttga ggtcgtgaag cagtttgtca
2161 ctggaattat agattccttg acaatttccc ccaaagccgc tcgagtgggg ctgctccagt
2221 attccacaca ggtccacaca gagttcactc tgagaaactt caactcagcc aaagacatga
2281 aaaaagccgt ggcccacatg aaatacatgg gaaagggctc tatgactggg ctggccctga
2341 aacacatgtt tgagagaagt tttacccaag gagaaggggc caggcctttt tccacaaggg
2401 tgcccagagc agccattgtg ttcaccgacg gacgggctca ggatgacgtc tccgagtggg
2461 ccagtaaagc caaggccaat ggtatcacta tgtatgctgt tggggtagga aaagccattg
2521 aggaggaact acaagagatt gcctctgagc ccacaaacaa gcatctcttc tatgccgaag
2581 acttcagcac aatggatgag ataagtgaaa aactcaagaa aggcatctgt gaagctctag
2641 aagactccga tggaagacag gactctccag caggggaact gccaaaaacg gtccaacagc
2701 caacagaatc tgagccagtc accataaata tccaagacct actttcctgt tctaattttg
2761 cagtgcaaca cagatatctg tttgaagaag acaatctttt acggtctaca caaaagcttt
2821 cccattcaac aaaaccttca ggaagccctt tggaagaaaa acacgatcaa tgcaaatgtg
2881 aaaaccttat aatgttccag aaccttgcaa acgaagaagt aagaaaatta acacagcgct
2941 tagaagaaat gacacagaga atggaagccc tggaaaatcg cctgagatac agatgaagat
3001 tagaaatcgc gacacatttg tagtcattgt atcacggatt acaatgaacg cagtgcagag
3061 ccccaaagct caggctattg ttaaatcaat aatgttgtga agtaaaacaa tcagtactga
3121 gaaacctggt ttgccacaga acaaagacaa gaagtataca ctaacttgta taaatttatc
3181 taggaaaaaa atccttcaga attctaagat gaatttacca ggtgagaatg aataagctat
3241 gcaaggtatt ttgtaatata ctgtggacac aacttgcttc tgcctcatcc tgccttagtg
3301 tgcaatctca tttgactata cgataaagtt tgcacagtct tacttctgta gaacactggc
3361 cataggaaat gctgtttttt tgtattggac tttaccttga tatatgtata tggatgtatg
3421 cataaaatca taggacatat gtacttgtgg aacaagttgg attttttata caatattaaa
3481 attcaccact tcagag
//