LOCUS HSU65090 5790 bp mRNA linear HUM 30-JUL-2002
DEFINITION Homo sapiens carboxypeptidase D mRNA, complete cds.
ACCESSION U65090
VERSION U65090.3
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5790)
AUTHORS McGwire,G.B., Tan,F., Michel,B., Rehli,M. and Skidgel,R.A.
TITLE Identification of a membrane-bound carboxypeptidase as the
mammalian homolog of duck gp180, a hepatitis B virus-binding
protein
JOURNAL Life Sci. 60 (10), 715-724 (1997)
PUBMED 9064476
REFERENCE 2 (bases 1 to 5790)
AUTHORS Tan,F., Rehli,M., Krause,S.W. and Skidgel,R.A.
TITLE Sequence of human carboxypeptidase D reveals it to be a member of
the regulatory carboxypeptidase family with three tandem active
site domains
JOURNAL Biochem. J. 327 (Pt 1), 81-87 (1997)
PUBMED 9355738
REFERENCE 3 (bases 1 to 5790)
AUTHORS Tan,F., Rehli,M. and Skidgel,R.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-1996) Pharmacology, Univ. of Illinois College of
Medicine at Chicago, 835 S. Wolcott (M/C 868), Chicago, IL 60612,
USA
REFERENCE 4 (bases 1 to 5790)
AUTHORS Tan,F., Rehli,M. and Skidgel,R.A.
TITLE Direct Submission
JOURNAL Submitted (18-JUL-2002) Pharmacology, Univ. of Illinois College of
Medicine at Chicago, 835 S. Wolcott (M/C 868), Chicago, IL 60612,
USA
REMARK Sequence update by submitter
REFERENCE 5 (bases 1 to 5790)
AUTHORS Tan,F., Rehli,M. and Skidgel,R.A.
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2002) Pharmacology, Univ. of Illinois College of
Medicine at Chicago, 835 S. Wolcott (M/C 868), Chicago, IL 60612,
USA
REMARK Sequence update by submitter
COMMENT On Jul 30, 2002 this sequence version replaced U65090.2.
FEATURES Location/Qualifiers
source 1..5790
/db_xref="H-InvDB:HIT000220872"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/note="6 clones were used to determine the sequence"
source 54..653
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="CPD-5'"
/tissue_type="placenta"
source 653..1113
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="152294"
/note="EST deposited under GenBank Accession Number
H04765; this clone provided by the IMAGE Consortium, LLNL"
source 653..1476
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="138429"
/note="EST deposited under GenBank Accession Number
R68612; this clone provided by the IMAGE Consortium, LLNL"
source 1063..3556
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="PCR25"
/tissue_type="liver"
source 1252..2559
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="cpdmak-01, mean size of 1.3 kb"
/cell_type="macrophage, differentiated in vitro"
/clone_lib="MAC library; M. Rehli et al. Biochem. Biophys.
Res. Commun. 217, 661-667 (1995)"
source 3511..5790
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="40521"
/note="EST deposited under GenBank Accession Number
R52214; this clone provided by the IMAGE Consortium, LLNL"
CDS 16..4158
/function="specifically cleaves C-terminal Arg or Lys
residues from peptides at an acidic pH optimum of 6.0 -
6.5"
/note="duck gp180 homolog"
/codon_start=1
/product="carboxypeptidase D"
/protein_id="AAC51775.2"
/translation="MASGRDERPHWRLGRLLLLMCLLLLGSSARAAHIKKAEATTTTT
SAGAEAAEGQFDRYYHEEELESALREAAAAGLPGLARLFSIGRSVEGRPLWVLRLTAG
LGSLIPEGDAGPDAAGPDAAGPLLPGRPQVKLVGNMHGDETVSRQVLIYLARELAAGY
RRGDPRLVRLLNTTDVYLLPSLNPDGFERAREGDCGFGDGGPSGASGRDNSRGRDLNR
SFPDQFSTGEPPALDEVPEVRALIEWIRRNKFVLSGNLHGGSVVASYPFDDSPEHKAT
GIYSKTSDDEVFKYLAKAYASNHPIMKTGEPHCPGDEDETFKDGITNGAHWYDVEGGM
QDYNYVWANCFEITLELSCCKYPPASQLRQEWENNRESLITLIEKVHIGVKGFVKDSI
TGSGLENATISVAGINHNITTGRFGDFYRLLVPGTYNLTVVLTGYMPLTVTNVVVKEG
PATEVDFSLRPTVTSVIPDTTEAVSTASTVAIPNILSGTSSSYQPIQPKDFHHHHFPD
MEIFLRRFANEYPNITRLYSLGKSVESRELYVMEISDNPGVHEPGEPEFKYIGNMHGN
EVVGRELLLNLIEYLCKNFGTDPEVTDLVHNTRIHLMPSMNPDGYEKSQEGDSISVIG
RNNSNNFDLNRNFPDQFVQITDPTQPETIAVMSWMKSYPFVLSANLHGGSLVVNYPFD
DDEQGLATYSKSPDDAVFQQIALSYSKENSQMFQGRPCKNMYPNEYFPHGITNGASWY
NVPGGMQDWNYLQTNCFEVTIELGCVKYPLEKELPNFWEQNRRSLIQFMKQVHQGVRG
FVLDATDGRGILNATISVAEINHPVTTYKTGDYWRLLVPGTYKITASARGYNPVTKNV
TVKSEGAIQVNFTLVRSSTDSNNESKKGKGASSSTNDASDPTTKEFETLIKDLSAENG
LESLMLRSSSNLALALYRYHSYKDLSEFLRGLVMNYPHITNLTNLGQSTEYRHIWSLE
ISNKPNVSEPEEPKIRFVAGIHGNAPVGTELLLALAEFLCLNYKKNPAVTQLVDRTRI
VIVPSLNPDGRERAQEKDCTSKIGQTNARGKDLDTDFTNNASQPETKAIIENLIQKQN
FSLSVALDGGSMLVTYPYDKPVQTVENKETLKHLASLYANNHPSMHMGQPSCPNKSDE
NIPGGVMRGAEWHSHLGSMKDYSVTYGHCPEITVYTSCCYFPSAARLPSLWADNKRSL
LSMLVEVHKGVHGFVKDKTGKPISKAVIVLNEGIKVQTKEGGYFHVLLAPGVHNIIAI
ADGYQQQHSQVFVHHDAASSVVIVFDTDNRIFGLPRELVVTVSGATMSALILTACIIW
CICSIKSNRHKDGFHRLRQHHDEYEDEIRMMSTGSKKSLLSHEFQDETDTEEETLYSS
KH"
sig_peptide 16..105
/inference="non-experimental evidence, no additional
details recorded"
misc_feature 3904..3981
/note="transmembrane region"
misc_feature 109..1404
/note="encodes carboxypeptidase domain 1"
misc_feature 529..531
/note="encodes potential glycosylation site"
misc_feature 1210..1212
/note="encodes potential glycosylation site"
misc_feature 1243..1245
/note="encodes potential glycosylation site"
misc_feature 1300..1302
/note="encodes potential glycosylation site"
misc_feature 1405..1506
/note="encodes putative connecting peptide"
misc_feature 1507..2625
/note="encodes carboxypeptidase domain 2"
misc_feature 1579..1581
/note="encodes potential glycosylation site"
misc_feature 1891..1893
/note="encodes potential glycosylation site"
misc_feature 2446..2448
/note="encodes potential glycosylation site"
misc_feature 2578..2580
/note="encodes potential glycosylation site"
misc_feature 2614..2616
/note="encodes potential glycosylation site"
misc_feature 2626..2712
/note="encodes putative connecting peptide"
misc_feature 2650..2652
/note="encodes potential glycosylation site"
misc_feature 2713..3912
/note="encodes carboxypeptidase domain 3"
misc_feature 2878..2880
/note="encodes potential glycosylation site"
misc_feature 2947..2949
/note="encodes potential glycosylation site"
misc_feature 3223..3225
/note="encodes potential glycosylation site"
misc_feature 3277..3279
/note="encodes potential glycosylation site"
misc_feature 3439..3441
/note="encodes potential glycosylation site"
misc_feature 3991..4155
/note="putative cytoplasmic domain"
3'UTR 4159..5790
regulatory 5738..5743
/regulatory_class="polyA_signal_sequence"
polyA_site 5763
BASE COUNT 1677 a 1245 c 1301 g 1567 t
ORIGIN
1 cggcgctgct ggaagatggc gagcggccgg gacgagcggc cgcattggcg gctagggcgg
61 ctcctgttgc tcatgtgcct gctgctgctg gggagctcgg cccgggcggc tcacatcaag
121 aaggcggagg cgactaccac aactacgagc gcgggcgccg aggcggccga gggccagttc
181 gaccgctact accacgaaga ggagttggag tcggcgctga gggaggcggc ggccgcgggc
241 ctccccggcc tggcccgcct ctttagcatc ggccgctcgg tggaaggccg gccgctgtgg
301 gtgcttcgcc tcaccgccgg cctggggtcg ctaatccctg agggcgacgc ggggcctgac
361 gctgccgggc ccgacgctgc ggggccgctg ctgcccggcc ggccccaggt gaagctggtg
421 ggcaacatgc atggcgacga gaccgtgtcg cgccaggtgt tgatctactt ggcccgcgag
481 ctggcggccg gctaccgccg cggggacccg cgcctggtcc gcctgctcaa caccaccgac
541 gtgtacctgc tgcccagcct caaccccgat ggcttcgagc gtgcccgcga gggcgactgt
601 ggcttcggcg acggcggccc gtccggggcc agcggccgcg acaatagtcg cggccgcgac
661 ctcaaccgaa gctttcccga ccagtttagc accggcgaac cccccgccct ggacgaggtg
721 cccgaggtgc gcgccctcat cgagtggatc cgcaggaaca agtttgtgct ttctggaaat
781 ctgcatggtg gctcagtggt agcaagctat ccttttgatg attctccaga acataaggcc
841 actggaatct atagcaaaac ctcagatgat gaagtattta aatacttggc aaaagcttat
901 gcttcaaacc accccataat gaaaactggt gagcctcatt gtccaggaga tgaagacgag
961 actttcaaag atggaatcac aaacggcgca cattggtatg atgtggaagg tggtatgcaa
1021 gattacaatt atgtgtgggc caactgtttt gagatcacat tagaactgtc ttgttgcaag
1081 tacccacctg cttcacagct tcgacaggaa tgggagaaca atcgtgagtc tttgatcaca
1141 ttgattgaaa aggttcacat tggagtgaaa ggatttgtta aagattccat aacaggatct
1201 gggttagaga atgcaaccat ctcagtggct ggtattaatc ataatatcac aacaggcaga
1261 tttggtgatt tctaccgatt acttgttcct ggaacttaca accttacagt agttttaact
1321 gggtatatgc cattgactgt tactaatgta gtggtgaaag aaggaccagc cacagaggtg
1381 gatttttctc ttaggccaac tgtaacttca gtaatccctg acacgacaga ggctgtatca
1441 actgctagca cagttgctat acctaatatt ctttctggaa catcatcctc ctaccagcca
1501 attcagccaa aggactttca ccaccaccat ttccctgata tggaaatctt cttgagaagg
1561 tttgccaatg aatatcctaa cattacccgg ctttattcct tgggaaaatc agtagagtca
1621 agagaacttt atgtgatgga gatatctgat aatccgggtg tccatgaacc aggtgaacca
1681 gaatttaagt acattggaaa tatgcatgga aatgaagtgg ttggaagaga actgctgttg
1741 aacctcatag aatacctttg taagaacttt ggaacagacc ctgaagtcac agatttggtt
1801 cataacacta gaattcacct tatgccatcc atgaatcctg atgggtatga aaagtcccag
1861 gaaggagatt caataagtgt aattggcaga aacaacagca acaactttga cctgaaccga
1921 aatttcccag accagtttgt tcagatcaca gatcctacgc aaccagaaac tattgctgta
1981 atgagctgga tgaagtccta tccatttgta ctttcagcaa acctgcatgg aggttctttg
2041 gtggttaact acccttttga tgatgatgaa caaggacttg ccacatatag taaatcacca
2101 gatgatgctg tgttccaaca aatagcactt tcttattcca aggaaaattc ccagatgttt
2161 caaggtagac cttgcaagaa tatgtatcct aatgaatatt ttcctcatgg aataacaaat
2221 ggagctagtt ggtataatgt gccaggagga atgcaggact ggaactattt acaaacaaat
2281 tgctttgaag tgactattga actaggttgt gtgaaatatc cacttgagaa agagctgcca
2341 aacttttggg aacagaatcg aagatcacta atccagttta tgaaacaggt tcatcagggc
2401 gtcagaggat ttgttctaga tgccacagat ggcaggggta tattaaatgc caccattagt
2461 gttgctgaga ttaatcaccc agtgactact tacaaaactg gagattactg gcgtctcttg
2521 gttccaggaa cttataaaat cacagcatct gctcgagggt ataatccagt taccaagaat
2581 gtgactgtca agagtgaagg cgctattcag gtcaacttca cacttgttcg atcctcaaca
2641 gattcaaaca atgaatcaaa gaaaggaaaa ggggctagca gcagcaccaa tgatgccagt
2701 gatccaacta ctaaagagtt tgaaacttta attaaagacc tttcagcgga gaatggtttg
2761 gaaagcctca tgttacgctc ctcctcaaat ctggctctgg ctctttatcg ataccattcc
2821 tacaaagact tatcagagtt tctgagagga cttgtaatga actatccaca tattacaaat
2881 cttaccaatt tgggacagag cactgaatat cgtcacattt ggtcccttga aatctccaat
2941 aagcccaatg tatctgagcc tgaagaacca aagattcgtt ttgttgctgg tatccatgga
3001 aatgcgccag ttggaactga actgcttttg gctctggcag aatttctctg cctgaactac
3061 aaaaagaacc cagctgttac ccaattggtt gacaggacta ggattgtgat tgtcccttct
3121 ctaaatccag atgggcgaga gagagctcaa gagaaagact gtacttcaaa aataggacaa
3181 acaaatgctc gtggcaaaga tttggataca gacttcacaa ataatgcctc ccaacctgag
3241 accaaagcca tcattgaaaa tttgattcaa aaacagaact ttagtctttc tgttgcctta
3301 gatggtggtt ccatgctggt cacatatcca tatgacaagc cagtacagac agtggaaaat
3361 aaagagactc tgaagcattt ggcatctctt tatgcaaata atcatccatc catgcacatg
3421 ggtcagccca gttgcccaaa taaatcagat gagaatattc caggaggagt aatgcgtgga
3481 gcagaatggc atagtcacct gggcagcatg aaggattata gtgtcaccta tggccattgt
3541 ccggaaatca cagtatacac aagctgctgt tactttccta gtgctgcacg actcccttcc
3601 ttgtgggcag acaataagag atctcttctt agtatgttag tggaggttca caagggagtt
3661 catggatttg ttaaagataa gactggaaag ccaatctcta aagcagtcat tgtacttaat
3721 gaaggaataa aggtacaaac aaaagaggga ggttatttcc atgtactctt agcgccaggt
3781 gtccataaca ttattgccat cgctgatggg taccagcaac aacattcaca ggtctttgtg
3841 catcatgatg cagctagttc tgtggtgata gtctttgaca cagataaccg gatatttggt
3901 ttgccaaggg agcttgtggt aactgtatca ggtgctacta tgtcggcatt gatcctaaca
3961 gcttgcatta tttggtgcat ctgctcaatc aagtctaata gacacaagga tggctttcat
4021 cggctcaggc agcatcatga tgagtatgaa gatgaaattc gcatgatgtc taccggctcc
4081 aagaagtccc tcctaagcca tgagttccag gatgaaacag acactgaaga ggaaacatta
4141 tattctagca aacattgaaa aacacatttt gcatatctcc cagcataagt accaagcaaa
4201 attacagttc ctcttgggag aacactgcat taagaagaga gactctcttg cttcttcaaa
4261 gagctttggg aaattaaatt gctaaatttg tattctctgt gaatttcact ggcagttttg
4321 aacttccctt ccttaaagta ctctaaacct ttaaaaaaaa atctgattta tgcagcagag
4381 atgggacagc cactttttct ttttaattta agatgagcta tttggagctt atgtaataat
4441 ggcataaagc caactagagg atgttgtatt ttgcacatca gatgtttact agtggcttta
4501 gtatttttct ttgttttaaa tggccaaaag aatccagaaa cattaaggca gggacagcag
4561 tcagaatcga cataaagctt taaaaactca aggttttttc aacctactga ggagtacttt
4621 tctctagttg ttaaatagct ggagtttttc ttattcaggt ttaatggagg ttgaattgat
4681 ttttaaacac atataacagt aggaaatgaa taaatgggct tctgcatttg gctttctacc
4741 tgttccaagg ctagatcgga actggtagac tacgctgtaa gcaggatttc actacctctc
4801 ttaaggttta gcaaacttct aaatagccca ttttaaggga gaacttacta actttattgt
4861 gaaaggtcta aatgcccact tgaatgaagc tgagagagag atctagcaaa agctaaaact
4921 catgttgtct atctttgaac ttggtaaaaa cccacaggtg ctgctgctta tatctgtgaa
4981 gcactagctt attctaggaa tgcctgattc tttaatattg cctaaatcgg aacctttttc
5041 tatgttgcac acatggtttt cagatgaccc agccatctac aagatctgaa ttctactgaa
5101 aatatctaga aatgtggaag agacctactt gcacattctt aacctgtatt tgaacacaaa
5161 atatctatac ttcatgctcc agcccaagcc tataccctgt aatagcatac tattattgaa
5221 atcgcttgac cggtcttgtt cacataggcc tctgggagtg atttggttct ttgccctaat
5281 gtttcatttg acggtctctt tttgatcaac caatttttct aaaagttcag tcgaaagctt
5341 ttaagtatag cttcctccct tgaaaaaaaa tgtaaactat gactgctgag tgataaaaca
5401 ctgtggtgtg aaagtgtcat cttcactgcc aatcaggcaa agaccggaaa gatttgcatt
5461 ttattatgtc tgtcttatca tgcaatggaa atgatgcttt ttgtaagtat gcatcttacc
5521 aatgatgtaa cggtttaata cctttgaatg ttttaataac caagttgctg ctgaacttat
5581 actaaatcag gggaccaaaa aacttgctct tatcttctca aattgtattc tatatccatt
5641 aatgtatcag ttatcccaaa gccttcaggt ggaggggttt accaccttcc taggtcgttc
5701 aaccaggttt tgtgaggaat gcattcaaag tggctttata aaagaagatt ttctttagca
5761 agaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//