LOCUS HSU65011 2148 bp mRNA linear HUM 21-MAR-1997
DEFINITION Human preferentially expressed antigen of melanoma (PRAME) mRNA,
complete cds.
ACCESSION U65011
VERSION U65011.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2148)
AUTHORS Ikeda,H., Lethe,B., Lehmann,F., Van Baren,N., Baurain,J.-F., De
Smet,C., Chambost,H., Vitale,M., Moretta,A., Boon,T. and
Coulie,P.G.
TITLE Characterization of an antigen that is recognized on a melanoma
showing partial HLA loss by CTL expressing an NK inhibitory
receptor
JOURNAL Immunity 6 (2), 199-208 (1997)
PUBMED 9047241
REFERENCE 2 (bases 1 to 2148)
AUTHORS Ikeda,H., Lethe,B., Baurain,J.-F. and Coulie,P.G.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-1996) Ludwig Institute for Cancer Research, 74
avenue Hippocrate UCL 7459, Brussels B-1200, Belgium
FEATURES Location/Qualifiers
source 1..2148
/db_xref="H-InvDB:HIT000220869"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/haplotype="A24 A28 B13 B44 Cw6 Cw7"
/cell_line="LB33-MEL.A"
/cell_type="melanoma cell"
gene 1..2148
/gene="PRAME"
CDS 236..1765
/gene="PRAME"
/note="encodes tumor antigen recognized by cytolytic T
lymphocytes"
/codon_start=1
/product="preferentially expressed antigen of melanoma"
/protein_id="AAC51160.1"
/translation="MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAA
LELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLD
GLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRK
VDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAM
PMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYI
SPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRL
SEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITD
DQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYED
IHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMP
N"
BASE COUNT 534 a 548 c 558 g 508 t
ORIGIN
1 gcttcagggt acagctcccc cgcagccaga agccgggcct gcagcccctc agcaccgctc
61 cgggacaccc cacccgcttc ccaggcgtga cctgtcaaca gcaacttcgc ggtgtggtga
121 actctctgag gaaaaaccat tttgattatt actctcagac gtgcgtggca acaagtgact
181 gagacctaga aatccaagcg ttggaggtcc tgaggccagc ctaagtcgct tcaaaatgga
241 acgaaggcgt ttgtggggtt ccattcagag ccgatacatc agcatgagtg tgtggacaag
301 cccacggaga cttgtggagc tggcagggca gagcctgctg aaggatgagg ccctggccat
361 tgccgccctg gagttgctgc ccagggagct cttcccgcca ctcttcatgg cagcctttga
421 cgggagacac agccagaccc tgaaggcaat ggtgcaggcc tggcccttca cctgcctccc
481 tctgggagtg ctgatgaagg gacaacatct tcacctggag accttcaaag ctgtgcttga
541 tggacttgat gtgctccttg cccaggaggt tcgccccagg aggtggaaac ttcaagtgct
601 ggatttacgg aagaactctc atcaggactt ctggactgta tggtctggaa acagggccag
661 tctgtactca tttccagagc cagaagcagc tcagcccatg acaaagaagc gaaaagtaga
721 tggtttgagc acagaggcag agcagccctt cattccagta gaggtgctcg tagacctgtt
781 cctcaaggaa ggtgcctgtg atgaattgtt ctcctacctc attgagaaag tgaagcgaaa
841 gaaaaatgta ctacgcctgt gctgtaagaa gctgaagatt tttgcaatgc ccatgcagga
901 tatcaagatg atcctgaaaa tggtgcagct ggactctatt gaagatttgg aagtgacttg
961 tacctggaag ctacccacct tggcgaaatt ttctccttac ctgggccaga tgattaatct
1021 gcgtagactc ctcctctccc acatccatgc atcttcctac atttccccgg agaaggaaga
1081 gcagtatatc gcccagttca cctctcagtt cctcagtctg cagtgcctgc aggctctcta
1141 tgtggactct ttatttttcc ttagaggccg cctggatcag ttgctcaggc acgtgatgaa
1201 ccccttggaa accctctcaa taactaactg ccggctttcg gaaggggatg tgatgcatct
1261 gtcccagagt cccagcgtca gtcagctaag tgtcctgagt ctaagtgggg tcatgctgac
1321 cgatgtaagt cccgagcccc tccaagctct gctggagaga gcctctgcca ccctccagga
1381 cctggtcttt gatgagtgtg ggatcacgga tgatcagctc cttgccctcc tgccttccct
1441 gagccactgc tcccagctta caaccttaag cttctacggg aattccatct ccatatctgc
1501 cttgcagagt ctcctgcagc acctcatcgg gctgagcaat ctgacccacg tgctgtatcc
1561 tgtccccctg gagagttatg aggacatcca tggtaccctc cacctggaga ggcttgccta
1621 tctgcatgcc aggctcaggg agttgctgtg tgagttgggg cggcccagca tggtctggct
1681 tagtgccaac ccctgtcctc actgtgggga cagaaccttc tatgacccgg agcccatcct
1741 gtgcccctgt ttcatgccta actagctggg tgcacatatc aaatgcttca ttctgcatac
1801 ttggacacta aagccaggat gtgcatgcat cttgaagcaa caaagcagcc acagtttcag
1861 acaaatgttc agtgtgagtg aggaaaacat gttcagtgag gaaaaaacat tcagacaaat
1921 gttcagtgag gaaaaaaagg ggaagttggg gataggcaga tgttgacttg aggagttaat
1981 gtgatctttg gggagataca tcttatagag ttagaaatag aatctgaatt tctaaaggga
2041 gattctggct tgggaagtac atgtaggagt taatccctgt gtagactgtt gtaaagaaac
2101 tgttgaaaat aaagagaagc aatgtgaagc aaaaaaaaaa aaaaaaaa
//