LOCUS HSU60519 3536 bp mRNA linear HUM 21-AUG-1996
DEFINITION Human apoptotic cysteine protease Mch4 (Mch4) mRNA, complete cds.
ACCESSION U60519
VERSION U60519.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2660)
AUTHORS Fernandes-Alnemri,T., Litwack,G. and Alnemri,E.S.
TITLE CPP32, a novel human apoptotic protein with homology to C. elegans
cell death protein CED-3 and mammalian interleukin-1-beta
converting enzyme
JOURNAL J. Biol. Chem. 269, 308761-308764 (1994)
REFERENCE 2 (bases 1 to 2309)
AUTHORS Fernandes-Alnemri,T.F., Takahashi,A., Armstrong,R., Krebs,J.,
Fritz,L., Tomaselli,K.J., Wang,L., Yu,Z., Croce,C.M., Salvesen,G.,
Earnshaw,W.C., Litwack,G. and Alnemri,E.S.
TITLE Mch3, a novel human apoptotic cysteine protease highly related to
CPP32
JOURNAL Cancer Res. 55 (24), 6045-6052 (1995)
PUBMED 8521391
REFERENCE 3 (bases 1 to 3536)
AUTHORS Fernandes-Alnemri,T.F., Armstrong,R.C., Krebs,J., Srinivasula,S.M.,
Wang,L., Bullrich,F., Fritz,L.C., Trapani,J.A., Tomaselli,K.J.,
Litwack,G. and Alnemri,E.S.
TITLE In vitro activation of CPP32 and Mch3 by Mch4, a novel human
apoptotic cysteine protease containing two FADD-like domains
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 93 (15), 7464-7469 (1996)
PUBMED 8755496
REFERENCE 4 (bases 1 to 3536)
AUTHORS Alnemri,E.S.
TITLE Direct Submission
JOURNAL Submitted (11-JUN-1996) Pharmacology, Thomas Jefferson University,
Jefferson Cancer Institute, 233, S. Tenth Street, Philadelphia, PA
19107, USA
FEATURES Location/Qualifiers
source 1..3536
/db_xref="H-InvDB:HIT000220637"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="t96-18B.1"
/cell_line="Jurkat"
/cell_type="T-lymphocyte"
gene 1..3536
/gene="Mch4"
CDS 148..1587
/gene="Mch4"
/function="activates CPP32 and Mch3"
/experiment="experimental evidence, no additional details
recorded"
/note="a new member of the aspartate-specific cysteine
protease family; the prodomain of this protease contains
FADD-like death effector domains"
/codon_start=1
/product="apoptotic cysteine protease Mch4"
/protein_id="AAC50644.1"
/translation="MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCI
GLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEV
ERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQG
KIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQT
DVKTFLEALPRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGF
TVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREI
MSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEAD
FLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRHEDILSILTAVNDDVSRRVDK
QGTKKQMPQPAFTLRKKLVFPVPLDALSI"
polyA_site 3536
/gene="Mch4"
BASE COUNT 944 a 820 c 818 g 954 t
ORIGIN
1 tgaagtctct tcccaagcaa atgggagctt ctttggacct tggagcacac agaggattct
61 actttcttta aaactttgtt ttcaggcaat ttccctgaga accgtttact tccagaagat
121 tggtggagct tgatctgaag gctggccatg aaatctcaag gtcaacattg gtattccagt
181 tcagataaaa actgtaaagt gagctttcgt gagaagcttc tgattattga ttcaaacctg
241 ggggtccaag atgtggagaa cctcaagttt ctctgcatag gattggtccc caacaagaag
301 ctggagaagt ccagctcagc ctcagatgtt tttgaacatc tcttggcaga ggatctgctg
361 agtgaggaag accctttctt cctggcagaa ctcctctata tcatacggca gaagaagctg
421 ctgcagcacc tcaactgtac caaagaggaa gtggagcgac tgctgcccac ccgacaaagg
481 gtttctctgt ttagaaacct gctctacgaa ctgtcagaag gcattgactc agagaactta
541 aaggacatga tcttccttct gaaagactcg cttcccaaaa ctgaaatgac ctccctaagt
601 ttcctggcat ttctagagaa acaaggtaaa atagatgaag ataatctgac atgcctggag
661 gacctctgca aaacagttgt acctaaactt ttgagaaaca tagagaaata caaaagagag
721 aaagctatcc agatagtgac acctcctgta gacaaggaag ccgagtcgta tcaaggagag
781 gaagaactag tttcccaaac agatgttaag acattcttgg aagccttacc gagggcagct
841 gtgtacagga tgaatcggaa ccacagaggc ctctgtgtca ttgtcaacaa ccacagcttt
901 acctccctga aggacagaca aggaacccat aaagatgctg agatcctgag tcatgtgttc
961 cagtggcttg ggttcacagt gcatatacac aataatgtga cgaaagtgga aatggagatg
1021 gtcctgcaga agcagaagtg caatccagcc catgccgacg gggactgctt cgtgttctgt
1081 attctgaccc atgggagatt tggagctgtc tactcttcgg atgaggccct cattcccatt
1141 cgggagatca tgtctcactt cacagccctg cagtgcccta gactggctga aaaacctaaa
1201 ctctttttca tccaggcctg ccaaggtgaa gagatacagc cttccgtatc catcgaagca
1261 gatgctctga accctgagca ggcacccact tccctgcagg acagtattcc tgccgaggct
1321 gacttcctac ttggtctggc cactgtccca ggctatgtat cctttcggca tgtggaggaa
1381 ggcagctggt atattcagtc tctgtgtaat catctgaaga aattggtccc aagacatgaa
1441 gacatcttat ccatcctcac tgctgtcaac gatgatgtga gtcgaagagt ggacaaacag
1501 ggaacaaaga aacagatgcc ccagcctgct ttcacactaa ggaaaaaact agtattccct
1561 gtgcccctgg atgcactttc aatatagcag agagtttttg ttggttctta gacctcaaac
1621 gaatcattgg gtataacctc cagcctcctg cccagcacag gaatcggtgg tctccacctg
1681 tcattctaga aacaggaaac accgtgtttt ctgacacagt caattctgat tttctttttc
1741 ttttgcaagt ctaaatgtta gaaaactttc tttttttgga gatagtctca ttctgtcacc
1801 cagactggag tgcagggggg caatcacggc tcactgtagt ctcgacctcc caggctcaag
1861 ctgtcctccc acctcagcct cccaagtagc tgagactaca ggtgtgtgtc catgcacagc
1921 taacttttta ttttttttgt ggagatgggg tttcactatg ttgcctaagc tggtctcaaa
1981 ctcctgggct caagcgatcc tcccacctca gcttctcaaa gttctgggac tacaggcatg
2041 aaatactgtg cctggcctgg ggaccaggtg cattttaagg ttccttggtg ttcaaaaacc
2101 acgttcttag cctagattga gcttagattg cctctctaga caactacccc ttagttataa
2161 ttctgtgtcc cctctgcatg cccttaaaca ttggacagtg aggtcacagt ccacccaccc
2221 tctctctgat ctcccccttc ctaagacttc tcttttgcac atctagtgag gtgaaaattt
2281 ggtctatgcc aggcccattt cctgcttttg tgtaaggaag gtgctcacat aggaagtttt
2341 tatttggtta gagacaggtt tccctgtagg aagatgatgg ctcatttaca ctcagctgct
2401 ctgcaagcag aaactttaca acctgatgtc atattccatt ttggactggg tgcggtgact
2461 catgcctgta atcccagtac tctgggaagc caaggcaggc agatcacttg aggtcaggag
2521 ttcgagacca gcctggccaa tacggcaaaa cctcatcatt actaaaaaca caaaaattag
2581 ccaggtgtgg cggcgagcac ctgtaatccc agctactcgg gaggctgaga caggagaatc
2641 tcttgaatcc aggaggcaga ggctgtggtg agccaagatg acacaactgc actccagctt
2701 gggcaacagg gcgagacctt gtttaaaaaa aaaattcaat attggggttg gaacatttca
2761 gttgccattg acagaacacc caattcaaat tgactgaagc aaagaaggga atttattgcc
2821 tctttcacat tgaaacccag gagtggataa cactggcttc aggcaaagct tgaatcagga
2881 ctcaatctac aggccagcac ctttctcttg gccggatgtc ctcagggctg gcagatgcag
2941 tagactgcag tggacagtcc ccaccttgtt actgctacta cactttgctc ctctggccca
3001 aggcatgagg agagaggctg tgtcagaaac tgaagctgtt ctcaggatca ctgggctctt
3061 cttggcagag gggatgtctg gcttgcctga agggagtggc tctgtaagga cgccttgatg
3121 ctttcttcat taagattttg agcattttta cgtacttgag cttttttttt tttttttttc
3181 aatttctaga ggaacttttt ctctgttaat tcctggaact gtattttgaa tccttaaagg
3241 tgagccctca tagggagatc caaagtcctg tggttaacgc cttcatttat agatgaggca
3301 gctgaggcct ggggatgtga acaacctgct cacagtcctc atttactgga tttgacttca
3361 gccaggtgaa ctggaatgcc ttggggcgtg gaagggcatt aggagtgttt catttgatat
3421 gtgaatgctc ataaaaaaat gtcaaggaat gaagaacaac aactctcagt ggtgcctgca
3481 tttataatta tttatgtgaa agtcaaattc atgtacagta aatttgttat aagaat
//