LOCUS HSU58996 1853 bp mRNA linear HUM 10-OCT-2001
DEFINITION Homo sapiens testis calpastatin mRNA, complete cds.
ACCESSION U58996
VERSION U58996.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1853)
AUTHORS Li,S., Liang,Z.G., Wang,G.Y., Yavetz,B., Kim,E.D. and Goldberg,E.
TITLE Molecular cloning and characterization of functional domains of a
human testis-specific isoform of calpastatin
JOURNAL Biol. Reprod. 63 (1), 172-178 (2000)
PUBMED 10859257
REFERENCE 2 (bases 1 to 1853)
AUTHORS O'Hern,P.A., Liang,Z.G., Wang,G.Y., Yavetz,B., Kim,E. and
Goldberg.,E.
TITLE A Novel Testis-specific Isoform of Calpastatin Detected with Serum
from an Infertile Patient
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 1853)
AUTHORS O'Hern,P.A., Liang,Z.G., Wang,G.Y., Yavetz,B., Kim,E. and
Goldberg.,E.
TITLE Direct Submission
JOURNAL Submitted (22-MAY-1996) Department of Biochemistry, Molecular
Biology and Cell Biology, Northwestern University, 2153 Sheridan
Road, Evanston, IL 60208, USA
REFERENCE 4 (bases 1 to 1853)
AUTHORS Goldberg,E.
TITLE Direct Submission
JOURNAL Submitted (15-APR-1998) Department of Biochemistry, Molecular
Biology and Cell Biology, Northwestern University, 2153 Sheridan
Road, Evanston, IL 60208, USA
REMARK Nucleotide and amino acid sequence updated by submitter
REFERENCE 5 (bases 1 to 1853)
AUTHORS Goldberg,E.
TITLE Direct Submission
JOURNAL Submitted (21-APR-1998) Department of Biochemistry, Molecular
Biology and Cell Biology, Northwestern University, 2153 Sheridan
Road, Evanston, IL 60208, USA
REMARK Nucleotide and amino acid sequences updated by submitter
REFERENCE 6 (bases 1 to 1853)
AUTHORS Goldberg,E.
TITLE Direct Submission
JOURNAL Submitted (11-JUN-1999) Department of Biochemistry, Molecular
Biology and Cell Biology, Northwestern University, 2153 Sheridan
Road, Evanston, IL 60208, USA
REMARK Sequence update by submitter
COMMENT On Jun 11, 1999 this sequence version replaced U58996.1.
FEATURES Location/Qualifiers
source 1..1853
/db_xref="H-InvDB:HIT000220565"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="testis"
CDS 67..1359
/function="inhibitor of calpain (calcium-dependent neutral
protease)"
/note="t-CAST; testis specific isoform of calpastatin"
/codon_start=1
/product="testis calpastatin"
/protein_id="AAC14353.1"
/translation="MGQFLSSTFLEGSPATVWHDKLCDGERRGAREAVRIFQDQAKAK
EEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRS
ECKEKPSKPTEKTEESKAAAPAPVSEAVSRTSMCSIQSAPPEPATLKGTVPDDAVEAL
ADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDNDGKPLL
PKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQA
GAPPRDTSSDKDLDDALDKLSDSLEQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDD
TIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQN
TAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD"
misc_feature 67..190
/note="encodes amino acids unique to testis isoform"
BASE COUNT 674 a 370 c 412 g 397 t
ORIGIN
1 gaattcgggg ggagtctccc tgacttccag caacaatcct tgagtctggg actgccctgg
61 cctaagatgg gccagtttct atcttcgact ttcttggagg gctcaccggc cacagtgtgg
121 cacgataagc tttgtgacgg tgaacgcaga ggagcaagag aagcagttcg tatcttccag
181 gaccaagcaa aagctaaaga agaaaaacta gagaagtgtg gtgaggatga tgaaacaatc
241 ccatctgagt acagattaaa accagccacg gataaagatg gaaaaccact attgccagag
301 cctgaagaaa aacccaagcc tcggagtgaa tcagaactca ttgatgaact ttcagaagat
361 tttgaccggt ctgaatgtaa agagaaacca tctaagccaa ctgaaaagac agaagaatct
421 aaggccgctg ctccagctcc tgtgtcggag gctgtgtctc ggacctccat gtgtagtata
481 cagtcagcac cccctgagcc ggctaccttg aagggcacag tgccagacga tgctgtagaa
541 gccttggctg atagcctggg gaaaaaggaa gcagatccag aagatggaaa acctgtgatg
601 gataaagtca aggagaaggc caaagaagaa gaccgtgaaa agcttggtga aaaagaagaa
661 acaattcctc ctgattatag attagaagag gtcaaggata atgatggaaa gccactcctg
721 ccaaaagagt ctaaggaaca gcttccaccc atgagtgaag acttccttct ggatgctttg
781 tctgaggact tctctggtcc acaaaatgct tcatctctta aatttgaaga tgctaaactt
841 gctgctgcca tctctgaagt ggtttcccaa accccagctt caacgaccca agctggagcc
901 ccaccccgtg atacctcgag tgacaaagac ctcgatgatg ccttggataa actctctgac
961 agtctagaac aaaggcagcc tgacccagat gagaacaaac caatggaaga taaagtaaag
1021 gaaaaagcta aagctgaaca tagagacaag cttggagaga gagatgacac tatcccacct
1081 gaatacagac atctcctgga tgataatgga caggacaaac cagtgaagcc acctacaaag
1141 aaatcagagg attcaaagaa acctgcagat gaccaagacc ccattgatgc tctctcagga
1201 gatctggaca gctgtccctc cactacagaa acctcacaga acacagcaaa ggataagtgc
1261 aagaaggctg cttccagctc caaagcacct aagaatggag gtaaagcgaa ggattcagca
1321 aagacaacag aggaaacttc caagccaaaa gatgactaaa gaaatacaag ttaaggtatc
1381 tggtatctgc atgtaaaatc ttcagctggt ggatggtgac ttttgaagaa caaaaggctt
1441 tggcaacaga aaacaattgt tctgggtgat ttctagaatg gtttttgttg agtctctgaa
1501 catcctaaat attggtttgt tattcttttc cagaaagaaa atgaatttga ctggttcacc
1561 tgtgtactga gtattgataa actttgaatt tttttaattg ccttcaattg ggagagaaag
1621 ctttatattt gtgtgaggac ttctgcatct tacagtcagc acagaacaca ctgagacttg
1681 aatcaagtca gcaacagagc aaaataaagg ttagataagt ccttgtgtag caaatttcga
1741 gcataagaaa taaaatctaa ttaattctta gggtaaaaaa aaaaaaaaaa aaaaaaaaaa
1801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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