LOCUS HSU48959 5926 bp mRNA linear HUM 14-MAR-2000
DEFINITION Homo sapiens myosin light chain kinase (MLCK) mRNA, complete cds.
ACCESSION U48959
VERSION U48959.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5926)
AUTHORS Garcia,J.G.N., Lazar,V., Gallagher,P.J., Patterson,C.E. and
Verin,A.D.
TITLE Endothelial cells contain a novel nonmuscle myosin light chain
kinase: DNA sequence and role in barrier function
JOURNAL Biochem. Biophys. Res. Commun. 1, 1-1 (1996)
REFERENCE 2 (bases 1 to 5926)
AUTHORS Garcia,J.G., Lazar,V., Gilbert-McClain,L.I., Gallagher,P.J. and
Verin,A.D.
TITLE Myosin light chain kinase in endothelium: molecular cloning and
regulation
JOURNAL Am. J. Respir. Cell Mol. Biol. 16 (5), 489-494 (1997)
PUBMED 9160829
REFERENCE 3 (bases 1 to 5926)
AUTHORS Lazar,V.
TITLE Direct Submission
JOURNAL Submitted (12-FEB-1996) Virginie Lazar, Pulmonary/Medicine, Indiana
University School of Medicine, 1001 W. 10th Street, OPW 425,
Indianapolis, IN 46202, USA
REFERENCE 4 (bases 1 to 5926)
AUTHORS Birukov,K.G. and Garcia,J.G.N.
TITLE Direct Submission
JOURNAL Submitted (14-MAR-2000) Pulmonary and Critical Care Division, Johns
Hopkins University School of Medicine, 501 Hopkins Bayview Circle,
Baltimore, MD 21224, USA
REMARK Sequence update by submitter
COMMENT On Mar 14, 2000 this sequence version replaced U48959.1.
FEATURES Location/Qualifiers
source 1..5926
/db_xref="H-InvDB:HIT000220074"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_type="endothelial; primary culture"
/tissue_type="umbilical vein"
gene 1..5926
/gene="MLCK"
CDS 120..5864
/gene="MLCK"
/function="catalyzes the phosphorylation of the 20 kDa
regulatory light chains of the myosin protein"
/note="protein kinase"
/codon_start=1
/product="myosin light chain kinase"
/protein_id="AAC18423.2"
/translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCI
KEGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRG
KYTCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSASAVETRPSIWGECP
PKFATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVL
EIHGVNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVT
NVISKESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQ
TPVLQKTSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQ
DVVSKAANRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEVSGIPKPEVAWFLE
GTPVRRQEGSIEVYEDAGSHYLCLLKARTRDSGTYSCTASNAQGQVSCSWTLQVERLA
VMEVAPSFSSVLKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVA
ELHIQDALPEDHGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPI
FLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLWI
QEVFPEDTGTYTCEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLI
SCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKN
RVGECSCQVSLMLQNSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPAR
GQGWLEEEDGEDVRGVLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDD
LKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVP
PPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAK
PAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNE
KRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIIL
SQEGSLCSVSIEKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSR
RPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVT
GTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQ
AQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTW
KELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVE
VSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKF
FKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIID
EDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLAR
RLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNE
TLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNM
EAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAE
KLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVW
FKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVE
TMEEGEGEGEEEEE"
BASE COUNT 1540 a 1582 c 1713 g 1091 t
ORIGIN
1 ccggctgcct ctgctgcagt tcagagcaac ttcaggagct tcccagccga gagcttcagg
61 acgcctttcc tgtcccactg gcccagttgc cacaacaaac aacagagaag acggtgacca
121 tgggggatgt gaagctggtt gcctcgtcac acatttccaa aacctccctc agtgtggatc
181 cctcaagagt tgactccatg cccctgacag aggcccctgc tttcattttg ccccctcgga
241 acctctgcat caaagaagga gccaccgcca agttcgaagg gcgggtccgg ggttacccag
301 agccccaggt gacatggcac agaaacgggc aacccatcac cagcgggggc cgcttcctgc
361 tggattgcgg catccggggg actttcagcc ttgtgattca tgctgtccat gaggaggaca
421 ggggaaagta tacctgtgaa gccaccaatg gcagtggtgc tcgccaggtg acagtggagt
481 tgacagtaga aggaagtttt gcgaagcagc ttggtcagcc tgttgtttcc aaaaccttag
541 gggatagatt ttcagcttca gcagtggaga cccgtcctag catctggggg gagtgcccac
601 caaagtttgc taccaagctg ggccgagttg tggtcaaaga aggacagatg ggacgattct
661 cctgcaagat cactggccgg ccccaaccgc aggtcacctg gctcaaggga aatgttccac
721 tgcagccgag tgcccgtgtg tctgtgtctg agaagaacgg catgcaggtt ctggaaatcc
781 atggagtcaa ccaagatgac gtgggagtgt acacgtgcct ggtggtgaac gggtcgggga
841 aggcctcgat gtcagctgaa ctttccatcc aaggtttgga cagtgccaat aggtcatttg
901 tgagagaaac aaaagccacc aattcagatg tcaggaaaga ggtgaccaat gtaatctcaa
961 aggagtcgaa gctggacagt ctggaggctg cagccaaaag caagaactgc tccagccccc
1021 agagaggtgg ctccccaccc tgggctgcaa acagccagcc tcagccccca agggagtcca
1081 agctggagtc atgcaaggac tcgcccagaa cggccccgca gaccccggtc cttcagaaga
1141 cttccagctc catcaccctg caggccgcaa gagttcagcc ggaaccaaga gcaccaggcc
1201 tgggggtcct atcaccttct ggagaagaga ggaagaggcc agctcctccc cgtccagcca
1261 ccttccccac caggcagcct ggcctgggga gccaagatgt tgtgagcaag gctgctaaca
1321 ggagaatccc catggagggc cagagggatt cagcattccc caaatttgag agcaagcccc
1381 aaagccagga ggtcaaggaa aatcaaactg tcaagttcag atgtgaagtt tccgggattc
1441 caaagcctga agtggcctgg ttcctggaag gcacccccgt gaggagacag gaaggcagca
1501 ttgaggttta tgaagatgct ggctcccatt acctctgcct gctgaaagcc cggaccaggg
1561 acagtgggac atacagctgc actgcttcca acgcccaagg ccaggtgtcc tgtagctgga
1621 ccctccaagt ggaaaggctt gccgtgatgg aggtggcccc ctccttctcc agtgtcctga
1681 aggactgcgc tgttattgag ggccaggatt ttgtgctgca gtgctccgta cgggggaccc
1741 cagtgccccg gatcacttgg ctgctgaatg ggcagcccat ccagtacgct cgctccacct
1801 gcgaggccgg cgtggctgag ctccacatcc aggatgccct gccggaggac catggcacct
1861 acacctgcct agctgagaat gccttggggc aggtgtcctg cagcgcctgg gtcaccgtcc
1921 atgaaaagaa gagtagcagg aagagtgagt accttctgcc tgtggctccc agcaagccca
1981 ctgcacccat cttcctgcag ggcctctctg atctcaaagt catggatgga agccaggtca
2041 ctatgactgt ccaagtgtca gggaatccac cccctgaagt catctggctg cacaatggga
2101 atgagatcca agagtcagag gacttccact ttgaacagag aggaactcag cacagccttt
2161 ggatccagga agtgttcccg gaggacacgg gcacgtacac ctgcgaggcc tggaacagcg
2221 ctggagaggt ccgcacccag gccgtgctca cggtacaaga gcctcacgat ggcacccagc
2281 cctggttcat cagtaagcct cgctcagtga cagcctccct gggccagagt gtcctcatct
2341 cctgcgccat agctggtgac ccctttccta ccgtgcactg gctcagagat ggcaaagccc
2401 tctgcaaaga cactggccac ttcgaggtgc ttcagaatga ggacgtgttc accctggttc
2461 taaagaaggt gcagccctgg catgccggcc agtatgagat cctgctcaag aaccgggttg
2521 gcgaatgcag ttgccaggtg tcactgatgc tacagaacag ctctgccaga gcccttccac
2581 gggggaggga gcctgccagc tgcgaggacc tctgtggtgg aggagttggt gctgatggtg
2641 gtggtagtga ccgctatggg tccctgaggc ctggctggcc agcaagaggg cagggttggc
2701 tagaggagga agacggcgag gacgtgcgag gggtgctgaa gaggcgcgtg gagacgaggc
2761 agcacactga ggaggcgatc cgccagcagg aggtggagca gctggacttc cgagacctcc
2821 tggggaagaa ggtgagtaca aagaccctat cggaagacga cctgaaggag atcccggccg
2881 agcagatgga tttccgtgcc aacctgcagc ggcaagtgaa gccaaagact gtgtctgagg
2941 aagagaggaa ggtgcacagc ccccagcagg tcgattttcg ctctgtcctg gccaagaagg
3001 ggacttccaa gacccccgtg cctgagaagg tgccaccgcc aaaacctgcc accccggatt
3061 ttcgctcagt gctgggtggc aagaagaaat taccagcaga gaatggcagc agcagtgccg
3121 agaccctgaa tgccaaggca gtggagagtt ccaagcccct gagcaatgca cagccttcag
3181 ggcccttgaa acccgtgggc aacgccaagc ctgctgagac cctgaagcca atgggcaacg
3241 ccaagcctgc cgagaccctg aagcccatgg gcaatgccaa gcctgatgag aacctgaaat
3301 ccgctagcaa agaagaactc aagaaagacg ttaagaatga tgtgaactgc aagagaggcc
3361 atgcagggac cacagataat gaaaagagat cagagagcca ggggacagcc ccagccttca
3421 agcagaagct gcaagatgtt catgtggcag agggcaagaa gctgctgctc cagtgccagg
3481 tgtcttctga ccccccagcc accatcatct ggacgctgaa tggaaagacc ctcaagacca
3541 ccaagttcat catcctctcc caggaaggct cactctgctc cgtctccatc gagaaggcac
3601 tgcctgagga cagaggctta tacaagtgtg tagccaagaa tgacgctggc caggcggagt
3661 gctcctgcca agtcaccgtg gatgatgctc cagccagtga gaacaccaag gccccagaga
3721 tgaaatcccg gaggcccaag agctctcttc ctcccgtgct aggaactgag agtgatgcga
3781 ctgtgaaaaa gaaacctgcc cccaagacac ctccgaaggc agcaatgccc cctcagatca
3841 tccagttccc tgaggaccag aaggtacgcg caggagagtc agtggagctg tttggcaaag
3901 tgacaggcac tcagcccatc acctgtacct ggatgaagtt ccgaaagcag atccaggaaa
3961 gcgagcacat gaaggtggag aacagcgaga atggcagcaa gctcaccatc ctggccgcgc
4021 gccaggagca ctgcggctgc tacacactgc tggtggagaa caagctgggc agcaggcagg
4081 cccaggtcaa cctcactgtc gtggataagc cagacccccc agctggcaca ccttgtgcct
4141 ctgacattcg gagctcctca ctgaccctgt cctggtatgg ctcctcatat gatgggggca
4201 gtgctgtaca gtcctacagc atcgagatct gggactcagc caacaagacg tggaaggaac
4261 tagccacatg ccgcagcacc tctttcaacg tccaggacct gctgcctgac cacgaatata
4321 agttccgtgt acgtgcaatc aacgtgtatg gaaccagtga gccaagccag gagtctgaac
4381 tcacaacggt aggagagaaa cctgaagagc cgaaggatga agtggaggtg tcagatgatg
4441 atgagaagga gcccgaggtt gattaccgga cagtgacaat caatactgaa caaaaagtat
4501 ctgacttcta cgacattgag gagagattag gatctgggaa atttggacag gtctttcgac
4561 ttgtagaaaa gaaaactcga aaagtctggg cagggaagtt cttcaaggca tattcagcaa
4621 aagagaaaga gaatatccgg caggagatta gcatcatgaa ctgcctccac caccctaagc
4681 tggtccagtg tgtggatgcc tttgaagaaa aggccaacat cgtcatggtc ctggagatcg
4741 tgtcaggagg ggagctgttt gagcgcatca ttgacgagga ctttgagctg acggagcgtg
4801 agtgcatcaa gtacatgcgg cagatctcgg agggagtgga gtacatccac aagcagggca
4861 tcgtgcacct ggacctcaag ccggagaaca tcatgtgtgt caacaagacg ggcaccagga
4921 tcaagctcat cgactttggt ctggccagga ggctggagaa tgcggggtct ctgaaggtcc
4981 tctttggcac cccagaattt gtggctcctg aagtgatcaa ctatgagccc atcggctacg
5041 ccacagacat gtggagcatc ggggtcatct gctacatcct agtcagtggc ctttccccct
5101 tcatgggaga caacgataac gaaaccttgg ccaacgttac ctcagccacc tgggacttcg
5161 acgacgaggc attcgatgag atctccgacg atgccaagga tttcatcagc aatctgctga
5221 agaaagatat gaaaaaccgc ctggactgca cgcagtgcct tcagcatcca tggctaatga
5281 aagataccaa gaacatggag gccaagaaac tctccaagga ccggatgaag aagtacatgg
5341 caagaaggaa atggcagaaa acgggcaatg ctgtgagagc cattggaaga ctgtcctcta
5401 tggcaatgat ctcagggctc agtggcagga aatcctcaac agggtcacca accagcccgc
5461 tcaatgcaga aaaactagaa tctgaagaag atgtgtccca agctttcctt gaggctgttg
5521 ctgaggaaaa gcctcatgta aaaccctatt tctctaagac cattcgcgat ttagaagttg
5581 tggagggaag tgctgctaga tttgactgca agattgaagg atacccagac cccgaggttg
5641 tctggttcaa agatgaccag tcaatcaggg agtcccgcca cttccagata gactacgatg
5701 aggacgggaa ctgctcttta attattagtg atgtttgcgg ggatgacgat gccaagtaca
5761 cctgcaaggc tgtcaacagt cttggagaag ccacctgcac agcagagctc attgtggaaa
5821 cgatggagga aggtgaaggg gaaggggaag aggaagaaga gtgaaacaaa gccagagaaa
5881 agcagtttct aagtcatatt aaaaggacta tttctctcaa aatcca
//