LOCUS HSU40434 2114 bp mRNA linear HUM 19-JAN-1996
DEFINITION Human mesothelin or CAK1 antigen precursor mRNA, complete cds.
ACCESSION U40434
VERSION U40434.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2114)
AUTHORS Chang,K. and Pastan,I.
TITLE Molecular cloning of mesothelin, a differentiation antigen present
on mesothelium, mesotheliomas, and ovarian cancers
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 93 (1), 136-140 (1996)
PUBMED 8552591
REFERENCE 2 (bases 1 to 2114)
AUTHORS Chang,K.
TITLE Direct Submission
JOURNAL Submitted (09-NOV-1995) Kai Chang, Laboratory of Molecular Biology,
National Cancer Institute, Building 37, Room 4B19, 37 Convent
Drive, MSC4255, Bethesda, MD MD20892-4255, USA
FEATURES Location/Qualifiers
source 1..2114
/db_xref="H-InvDB:HIT000219720"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="16"
/clone="pcD3CAK1-9"
/cell_line="KB, HeLa, OVCAR-3"
/cell_type="HeLa"
/tissue_type="ovarian cancers and squamous cell
carcinoma."
CDS 100..1986
/codon_start=1
/product="mesothelin or CAK1 antigen precursor"
/protein_id="AAC50348.1"
/translation="MALQRLDPCWSCGDRPGSLLFLLFSLGWVHPARTLAGETGTESA
PLGGVLTTPHNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCL
AHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRL
LPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQ
EAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRD
PSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQM
DRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLET
LKALLEVDKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSL
SPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGG
APTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVR
DWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSVQETLSGTPCLLGPGPVLTVLALLLA
STLA"
sig_peptide 100..192
mat_peptide 193..1857
/product="mesothelin"
misc_feature 193..978
/note="encodes putative cleaved N-terminal portion of
mesothelin"
misc_feature 973..978
/note="encodes putative proteolytic site"
misc_feature 979..1983
/note="encodes putative membrane bound portion of
mesothelin, bearing epitope(s) recognized by MAb K1"
misc_feature 1858..1983
/note="encodes putative hydrophobic region for GPI
anchorage"
regulatory 2087..2093
/regulatory_class="polyA_signal_sequence"
/note="putative"
polyA_site 2114
BASE COUNT 361 a 722 c 661 g 370 t
ORIGIN
1 aggaattccg gtggccggcc actcccgtct gctgtgacgc gcggacagag agctaccggt
61 ggacccacgg tgcctccctc cctgggatct acacagacca tggccttgca acggctcgac
121 ccctgttggt cctgtgggga ccgccctggc agcctcctgt tcctgctctt cagcctcgga
181 tgggtgcatc ccgcgaggac cctggctgga gagacaggga cggagtctgc ccccctgggg
241 ggagtcctga caacccccca taacatttcc agcctctccc ctcgccaact ccttggcttc
301 ccgtgtgcgg aggtgtccgg cctgagcacg gagcgtgtcc gggagctggc tgtggccttg
361 gcacagaaga atgtcaagct ctcaacagag cagctgcgct gtctggctca ccggctctct
421 gagccccccg aggacctgga cgccctccca ttggacctgc tgctattcct caacccagat
481 gcgttctcgg ggccccaggc ctgcacccgt ttcttctccc gcatcacgaa ggccaatgtg
541 gacctgctcc cgaggggggc tcccgagcga cagcggctgc tgcctgcggc tctggcctgc
601 tggggtgtgc gggggtctct gctgagcgag gctgatgtgc gggctctggg aggcctggct
661 tgcgacctgc ctgggcgctt tgtggccgag tcggccgaag tgctgctacc ccggctggtg
721 agctgcccgg gacccctgga ccaggaccag caggaggcag ccagggcggc tctgcagggc
781 gggggacccc cctacggccc cccgtcgaca tggtctgtct ccacgatgga cgctctgcgg
841 ggcctgctgc ccgtgctggg ccagcccatc atccgcagca tcccgcaggg catcgtggcc
901 gcgtggcggc aacgctcctc tcgggaccca tcctggcggc agcctgaacg gaccatcctc
961 cggccgcggt tccggcggga agtggagaag acagcctgtc cttcaggcaa gaaggcccgc
1021 gagatagacg agagcctcat cttctacaag aagtgggagc tggaagcctg cgtggatgcg
1081 gccctgctgg ccacccagat ggaccgcgtg aacgccatcc ccttcaccta cgagcagctg
1141 gacgtcctaa agcataaact ggatgagctc tacccacaag gttaccccga gtctgtgatc
1201 cagcacctgg gctacctctt cctcaagatg agccctgagg acattcgcaa gtggaatgtg
1261 acgtccctgg agaccctgaa ggctttgctt gaagtcgaca aagggcacga aatgagtcct
1321 caggctcctc ggcggcccct cccacaggtg gccaccctga tcgaccgctt tgtgaaggga
1381 aggggccagc tagacaaaga caccctagac accctgaccg ccttctaccc tgggtacctg
1441 tgctccctca gccccgagga gctgagctcc gtgcccccca gcagcatctg ggcggtcagg
1501 ccccaggacc tggacacgtg tgacccaagg cagctggacg tcctctatcc caaggcccgc
1561 cttgctttcc agaacatgaa cgggtccgaa tacttcgtga agatccagtc cttcctgggt
1621 ggggccccca cggaggattt gaaggcgctc agtcagcaga atgtgagcat ggacttggcc
1681 acgttcatga agctgcggac ggatgcggtg ctgccgttga ctgtggctga ggtgcagaaa
1741 cttctgggac cccacgtgga gggcctgaag gcggaggagc ggcaccgccc ggtgcgggac
1801 tggatcctac ggcagcggca ggacgacctg gacacgctgg ggctggggct acagggcggc
1861 atccccaacg gctacctggt cctagacctc agcgtgcaag agaccctctc ggggacgccc
1921 tgcctcctag gacctggacc tgttctcacc gtcctggcac tgctcctagc ctccaccctg
1981 gcctgagggc cccactccct tgctggcccc agccctgctg gggatccccg cctggccagg
2041 agcaggcacg ggtgatcccc gttccacccc aagagaactc gcgctcagta aacgggaaca
2101 tgccccctgc agac
//