LOCUS HSU30246 4098 bp mRNA linear HUM 06-JAN-2005
DEFINITION Human bumetanide-sensitive Na-K-Cl cotransporter (NKCC1) mRNA,
complete cds.
ACCESSION U30246
VERSION U30246.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4098)
AUTHORS Payne,J.A., Xu,J.C., Haas,M., Lytle,C.Y., Ward,D. and Forbush,B.
III.
TITLE Primary structure, functional expression, and chromosomal
localization of the bumetanide-sensitive Na-K-Cl cotransporter in
human colon
JOURNAL J. Biol. Chem. 270 (30), 17977-17985 (1995)
PUBMED 7629105
REFERENCE 2 (bases 1 to 1212)
AUTHORS Gillen,C.M., Brill,S., Payne,J.A. and Forbush,B. III.
TITLE Molecular cloning and functional expression of the K-Cl
cotransporter from rabbit, rat, and human. A new member of the
cation-chloride cotransporter family
JOURNAL J. Biol. Chem. 271 (27), 16237-16244 (1996)
PUBMED 8663127
REFERENCE 3 (bases 1 to 4098)
AUTHORS Payne,J.A.
TITLE Direct Submission
JOURNAL Submitted (23-JUN-1995) John A. Payne, Human Physiology, University
of California, School of Medicine, Medical Sciences Bldg.1A #4138,
Davis, CA 95616, USA
FEATURES Location/Qualifiers
source 1..4098
/db_xref="H-InvDB:HIT000219025"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="TEF 1-1 and TEF 11a"
/cell_line="T84 cell line"
/tissue_type="colon tumor"
/clone_lib="Stratagene T84 library of J. Riordan"
gene 1..4098
/gene="NKCC1"
CDS 165..3803
/gene="NKCC1"
/codon_start=1
/product="bumetanide-sensitive Na-K-Cl cotransporter"
/protein_id="AAC50561.1"
/translation="MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPE
DAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAA
AAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAA
GVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTM
DAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYT
AESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTIT
GLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVV
ELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFV
IGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANIS
GDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSA
ACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPK
IFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFF
LASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYV
IVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAP
NSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYA
PVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQ
YGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKP
ITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLF
DDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMV
LGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKT
YRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNH
QSVLTFYS"
regulatory 4075..4080
/regulatory_class="polyA_signal_sequence"
/gene="NKCC1"
BASE COUNT 1090 a 890 c 1037 g 1081 t
ORIGIN
1 ggtggcctct gtggccgtcc aggctagcgg cggcccgcag gcggcgggga gaaagactct
61 ctcacctggt cttgcggctg tggccaccgc cggccagggg tgtggagggc gtgctgccgg
121 agacgtccgc cgggctctgc agttccgccg ggggtcgggc agctatggag ccgcggccca
181 cggcgccctc ctccggcgcc ccgggactgg ccggggtcgg ggagacgccg tcagccgctg
241 cgctggccgc agccagggtg gaactgcccg gcacggctgt gccctcggtg ccggaggatg
301 ctgcgcccgc gagccgggac ggcggcgggg tccgcgatga gggccccgcg gcggccgggg
361 acgggctggg cagacccttg gggcccaccc cgagccagag ccgtttccag gtggacctgg
421 tttccgagaa cgccgggcgg gccgctgctg cggcggcggc ggcggcggcg gcagcggcgg
481 cggctggtgc tggggcgggg gccaagcaga cccccgcgga cggggaagcc agcggcgaga
541 gcgagccagc taaaggcagc gaggaagcca agggccgctt ccgcgtgaac ttcgtggacc
601 cagctgcctc ctcgtcggct gaagacagcc tgtcagatgc tgccggggtc ggagtcgacg
661 ggcccaacgt gagcttccag aacggcgggg acacggtgct gagcgagggc agcagcctgc
721 actccggcgg cggcggcggc agtgggcacc accagcacta ctattatgat acccacacca
781 acacctacta cctgcgcacc ttcggccaca acaccatgga cgctgtgccc aggatcgatc
841 actaccggca cacagccgcg cagctgggcg agaagctgct ccggcctagc ctggcggagc
901 tccacgacga gctggaaaag gaaccttttg aggatggctt tgcaaatggg gaagaaagta
961 ctccaaccag agatgctgtg gtcacgtata ctgcagaaag taaaggagtc gtgaagtttg
1021 gctggatcaa gggtgtatta gtacgttgta tgttaaacat ttggggtgtg atgcttttca
1081 ttagattgtc atggattgtg ggtcaagctg gaataggtct atcagtcctt gtaataatga
1141 tggccactgt tgtgacaact atcacaggat tgtctacttc agcaatagca actaatggat
1201 ttgtaagagg aggaggagca tattatttaa tatctagaag tctagggcca gaatttggtg
1261 gtgcaattgg tctaatcttc gcctttgcca acgctgttgc agttgctatg tatgtggttg
1321 gatttgcaga aaccgtggtg gagttgctta aggaacattc catacttatg atagatgaaa
1381 tcaatgatat ccgaattatt ggagccatta cagtcgtgat tcttttaggt atctcagtag
1441 ctggaatgga gtgggaagca aaagctcaga ttgttctttt ggtgatccta cttcttgcta
1501 ttggtgattt cgtcatagga acatttatcc cactggagag caagaagcca aaagggtttt
1561 ttggttataa atctgaaata tttaatgaga actttgggcc cgattttcga gaggaagaga
1621 ctttcttttc tgtatttgcc atcttttttc ctgctgcaac tggtattctg gctggagcaa
1681 atatctcagg tgatcttgca gatcctcagt cagccatacc caaaggaaca ctcctagcca
1741 ttttaattac tacattggtt tacgtaggaa ttgcagtatc tgtaggttct tgtgttgttc
1801 gagatgccac tggaaacgtt aatgacacta tcgtaacaga gctaacaaac tgtacttctg
1861 cagcctgcaa attaaacttt gatttttcat cttgtgaaag cagtccttgt tcctatggcc
1921 taatgaacaa cttccaggta atgagtatgg tgtcaggatt tacaccacta atttctgcag
1981 gtatattttc agccactctt tcttcagcat tagcatccct agtgagtgct cccaaaatat
2041 ttcaggctct atgtaaggac aacatctacc cagctttcca gatgtttgct aaaggttatg
2101 ggaaaaataa tgaacctctt cgtggctaca tcttaacatt cttaattgca cttggattca
2161 tcttaattgc tgaactgaat gttattgcac caattatctc aaacttcttc cttgcatcat
2221 atgcattgat caatttttca gtattccatg catcacttgc aaaatctcca ggatggcgtc
2281 ctgcattcaa atactacaac atgtggatat cacttcttgg agcaattctt tgttgcatag
2341 taatgttcgt cattaactgg tgggctgcat tgctaacata tgtgatagtc cttgggctgt
2401 atatttatgt tacctacaaa aaaccagatg tgaattgggg atcctctaca caagccctga
2461 cttacctgaa tgcactgcag cattcaattc gtctttctgg agtggaagac cacgtgaaaa
2521 actttaggcc acagtgtctt gttatgacag gtgctccaaa ctcacgtcca gctttacttc
2581 atcttgttca tgatttcaca aaaaatgttg gtttgatgat ctgtggccat gtacatatgg
2641 gtcctcgaag acaagccatg aaagagatgt ccatcgatca agccaaatat cagcgatggc
2701 ttattaagaa caaaatgaag gcattttatg ctccagtaca tgcagatgac ttgagagaag
2761 gtgcacagta tttgatgcag gctgctggtc ttggtcgtat gaagccaaac acacttgtcc
2821 ttggatttaa gaaagattgg ttgcaagcag atatgaggga tgtggatatg tatataaact
2881 tatttcatga tgcttttgac atacaatatg gagtagtggt tattcgccta aaagaaggtc
2941 tggatatatc tcatcttcaa ggacaagaag aattattgtc atcacaagag aaatctcctg
3001 gcaccaagga tgtggtagta agtgtggaat atagtaaaaa gtccgattta gatacttcca
3061 aaccactcag tgaaaaacca attacacaca aagttgagga agaggatggc aagactgcaa
3121 ctcaaccact gttgaaaaaa gaatccaaag gccctattgt gcctttaaat gtagctgacc
3181 aaaagcttct tgaagctagt acacagtttc agaaaaaaca aggaaagaat actattgatg
3241 tctggtggct ttttgatgat ggaggtttga ccttattgat accttacctt ctgacgacca
3301 agaaaaaatg gaaagactgt aagatcagag tattcattgg tggaaagata aacagaatag
3361 accatgaccg gagagcgatg gctactttgc ttagcaagtt ccggatagac ttttctgata
3421 tcatggttct aggagatatc aataccaaac caaagaaaga aaatattata gcttttgagg
3481 aaatcattga gccatacaga cttcatgaag atgataaaga gcaagatatt gcagataaaa
3541 tgaaagaaga tgaaccatgg cgaataacag ataatgagct tgaactttat aagaccaaga
3601 cataccggca gatcaggtta aatgagttat taaaggaaca ttcaagcaca gctaatatta
3661 ttgtcatgag tctcccagtt gcacgaaaag gtgctgtgtc tagtgctctc tacatggcat
3721 ggttagaagc tctatctaag gacctaccac caatcctcct agttcgtggg aatcatcaga
3781 gtgtccttac cttctattca taaatgttct atacagtgga cagccctcca gaatggtact
3841 tcagtgccta gtgtagtaac ctgaaatctt caatgacaca ttaacatcac aatggcgaat
3901 ggtgactttt ctttcacgat ttcattaatt tgaaagcaca caggaaagct tgctccattg
3961 ataacgtgta tggagacttc ggttttagtc aattccatat ctcaatctta atggtgattc
4021 ttctctgttg aactgaagtt tgtgagagta gttttccttt gctacttgaa tagcaataaa
4081 agcgtgttaa ctttttgg
//