LOCUS HSU28946 4264 bp mRNA linear HUM 04-MAY-1996
DEFINITION Human G/T mismatch binding protein (GTBP) mRNA, complete cds.
ACCESSION U28946
VERSION U28946.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4264)
AUTHORS Palombo,F., Gallinari,P., Iaccarino,I., Lettieri,T., Hughes,M.,
D'Arrigo,A., Truong,O., Hsuan,J.J. and Jiricny,J.
TITLE GTBP, a 160-kilodalton protein essential for mismatch-binding
activity in human cells
JOURNAL Science 268 (5219), 1912-1914 (1995)
PUBMED 7604265
REFERENCE 2 (bases 1 to 4264)
AUTHORS Nicolaides,N.C., Palombo,F., Kinzler,K.W., Vogelstein,B. and
Jiricny,J.
TITLE Molecular cloning of the N-terminus of GTBP
JOURNAL Genomics 31 (3), 395-397 (1996)
PUBMED 8838326
REFERENCE 3 (bases 1 to 4264)
AUTHORS Jiricny,J.
TITLE Direct Submission
JOURNAL Submitted (12-JUN-1995) Josef Jiricny, Genetics Department,
Istituto di Ricerche di Biol. Molecolare P. Angeletti (IRBM), Via
Pontina Km 30.600, Pomezia, 00040, Italy
COMMENT On May 4, 1996 this sequence version replaced gi:902495.
FEATURES Location/Qualifiers
source 1..4264
/db_xref="H-InvDB:HIT000218941"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="2"
/map="2p16"
/clone="C1"
/cell_line="HeLa"
/clone_lib="HeLa S3 cDNA in lambda Uni-ZAP XR
(Stratagene)"
gene 1..4264
/gene="GTBP"
CDS 88..4170
/gene="GTBP"
/note="homolog of bacterial MutS proteins; binds to G/T
mismatches through heterodimerization with hMSH2; similar
to ORF YD8557.04c, probable DNA repair protein, from S.
cerevisiae chromosome IV cosmid 8557, PIR Accession Number
S51246; somatic mutations found in colon cancer"
/codon_start=1
/product="G/T mismatch binding protein"
/protein_id="AAC50461.1"
/translation="MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSP
GGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEG
YPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQ
KGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDK
SEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSD
EISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETK
NTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFD
ASTLYVPEDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLV
FMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRV
VRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLG
KFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQEGLIPG
SQFWDASKTLRTLLEEEYFREKLSDGIGVMLPQVLKGMTSESDSIGLTPGEKSELALS
ALGGCVFYLKKCLIDQELLSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTL
NNLEIFLNGTNGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDL
MVVPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKII
DFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLTVELNRWDT
AFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGI
GRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLK
DCMRRLFYNFDKNYKDWQSAVECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPF
LELKGSRHPCITKTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQ
AGLLAVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHA
TAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDYSQNVAV
RLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKA
REFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTLIKEL"
BASE COUNT 1249 a 840 c 1076 g 1099 t
ORIGIN
1 atttcccgcc agcaggagcc gcgcggtaga tgcggtgctt ttaggagctc cgtccgacag
61 aacggttggg ccttgccggc tgtcggtatg tcgcgacaga gcaccctgta cagcttcttc
121 cccaagtctc cggcgctgag tgatgccaac aaggcctcgg ccagggcctc acgcgaaggc
181 ggccgtgccg ccgctgcccc cggggcctct ccttccccag gcggggatgc ggcctggagc
241 gaggctgggc ctgggcccag gcccttggcg cgatccgcgt caccgcccaa ggcgaagaac
301 ctcaacggag ggctgcggag atcggtagcg cctgctgccc ccaccagttg tgacttctca
361 ccaggagatt tggtttgggc caagatggag ggttacccct ggtggccttg tctggtttac
421 aaccacccct ttgatggaac attcatccgc gagaaaggga aatcagtccg tgttcatgta
481 cagttttttg atgacagccc aacaaggggc tgggttagca aaaggctttt aaagccatat
541 acaggttcaa aatcaaagga agcccagaag ggaggtcatt tttacagtgc aaagcctgaa
601 atactgagag caatgcaacg tgcagatgaa gccttaaata aagacaagat taagaggctt
661 gaattggcag tttgtgatga gccctcagag ccagaagagg aagaagagat ggaggtaggc
721 acaacttacg taacagataa gagtgaagaa gataatgaaa ttgagagtga agaggaagta
781 cagcctaaga cacaaggatc taggcgaagt agccgccaaa taaaaaaacg aagggtcata
841 tcagattctg agagtgacat tggtggctct gatgtggaat ttaagccaga cactaaggag
901 gaaggaagca gtgatgaaat aagcagtgga gtgggggata gtgagagtga aggcctgaac
961 agccctgtca aagttgctcg aaagcggaag agaatggtga ctggaaatgg ctctcttaaa
1021 aggaaaagct ctaggaagga aacgccctca gccaccaaac aagcaactag catttcatca
1081 gaaaccaaga atactttgag agctttctct gcccctcaaa attctgaatc ccaagcccac
1141 gttagtggag gtggtgatga cagtagtcgc cctactgttt ggtatcatga aactttagaa
1201 tggcttaagg aggaaaagag aagagatgag cacaggagga ggcctgatca ccccgatttt
1261 gatgcatcta cactctatgt gcctgaggat ttcctcaatt cttgtactcc tgggatgagg
1321 aagtggtggc agattaagtc tcagaacttt gatcttgtca tctgttacaa ggtggggaaa
1381 ttttatgagc tgtaccacat ggatgctctt attggagtca gtgaactggg gctggtattc
1441 atgaaaggca actgggccca ttctggcttt cctgaaattg catttggccg ttattcagat
1501 tccctggtgc agaagggcta taaagtagca cgagtggaac agactgagac tccagaaatg
1561 atggaggcac gatgtagaaa gatggcacat atatccaagt atgatagagt ggtgaggagg
1621 gagatctgta ggatcattac caagggtaca cagacttaca gtgtgctgga aggtgatccc
1681 tctgagaact acagtaagta tcttcttagc ctcaaagaaa aagaggaaga ttcttctggc
1741 catactcgtg catatggtgt gtgctttgtt gatacttcac tgggaaagtt tttcataggt
1801 cagttttcag atgatcgcca ttgttcgaga tttaggactc tagtggcaca ctatccccca
1861 gtacaagttt tatttgaaaa aggaaatctc tcaaaggaaa ctaaaacaat tctaaagagt
1921 tcattgtcct gttctcttca ggaaggtctg atacccggct cccagttttg ggatgcatcc
1981 aaaactttga gaactctcct tgaggaagaa tattttaggg aaaagctaag tgatggcatt
2041 ggggtgatgt taccccaggt gcttaaaggt atgacttcag agtctgattc cattgggttg
2101 acaccaggag agaaaagtga attggccctc tctgctctag gtggttgtgt cttctacctc
2161 aaaaaatgcc ttattgatca ggagctttta tcaatggcta attttgaaga atatattccc
2221 ttggattctg acacagtcag cactacaaga tctggtgcta tcttcaccaa agcctatcaa
2281 cgaatggtgc tagatgcagt gacattaaac aacttggaga tttttctgaa tggaacaaat
2341 ggttctactg aaggaaccct actagagagg gttgatactt gccatactcc ttttggtaag
2401 cggctcctaa agcaatggct ttgtgcccca ctctgtaacc attatgctat taatgatcgt
2461 ctagatgcca tagaagacct catggttgtg cctgacaaaa tctccgaagt tgtagagctt
2521 ctaaagaagc ttccagatct tgagaggcta ctcagtaaaa ttcataatgt tgggtctccc
2581 ctgaagagtc agaaccaccc agacagcagg gctataatgt atgaagaaac tacatacagc
2641 aagaagaaga ttattgattt tctttctgct ctggaaggat tcaaagtaat gtgtaaaatt
2701 atagggatca tggaagaagt tgctgatggt tttaagtcta aaatccttaa gcaggtcatc
2761 tctctgcaga caaaaaatcc tgaaggtcgt tttcctgatt tgactgtaga attgaaccga
2821 tgggatacag cctttgacca tgaaaaggct cgaaagactg gacttattac tcccaaagca
2881 ggctttgact ctgattatga ccaagctctt gctgacataa gagaaaatga acagagcctc
2941 ctggaatacc tagagaaaca gcgcaacaga attggctgta ggaccatagt ctattggggg
3001 attggtagga accgttacca gctggaaatt cctgagaatt tcaccactcg caatttgcca
3061 gaagaatacg agttgaaatc taccaagaag ggctgtaaac gatactggac caaaactatt
3121 gaaaagaagt tggctaatct cataaatgct gaagaacgga gggatgtatc attgaaggac
3181 tgcatgcggc gactgttcta taactttgat aaaaattaca aggactggca gtctgctgta
3241 gagtgtatcg cagtgttgga tgttttactg tgcctggcta actatagtcg agggggtgat
3301 ggtcctatgt gtcgcccagt aattctgttg ccggaagata cccccccctt cttagagctt
3361 aaaggatcac gccatccttg cattacgaag actttttttg gagatgattt tattcctaat
3421 gacattctaa taggctgtga ggaagaggag caggaaaatg gcaaagccta ttgtgtgctt
3481 gttactggac caaatatggg gggcaagtct acgcttatga gacaggctgg cttattagct
3541 gtaatggccc agatgggttg ttacgtccct gctgaagtgt gcaggctcac accaattgat
3601 agagtgttta ctagacttgg tgcctcagac agaataatgt caggtgaaag tacatttttt
3661 gttgaattaa gtgaaactgc cagcatactc atgcatgcaa cagcacattc tctggtgctt
3721 gtggatgaat taggaagagg tactgcaaca tttgatggga cggcaatagc aaatgcagtt
3781 gttaaagaac ttgctgagac tataaaatgt cgtacattat tttcaactca ctaccattca
3841 ttagtagaag attattctca aaatgttgct gtgcgcctag gacatatggc atgcatggta
3901 gaaaatgaat gtgaagaccc cagccaggag actattacgt tcctctataa attcattaag
3961 ggagcttgtc ctaaaagcta tggctttaat gcagcaaggc ttgctaatct cccagaggaa
4021 gttattcaaa agggacatag aaaagcaaga gaatttgaga agatgaatca gtcactacga
4081 ttatttcggg aagtttgcct ggctagtgaa aggtcaactg tagatgctga agctgtccat
4141 aaattgctga ctttgattaa ggaattatag actgactaca ttggaagctt tgagttgact
4201 tctgaccaaa ggtggtaaat tcagacaaca ttatgatcta ataaacttta ttttttaaaa
4261 atga
//