LOCUS HSU27109 4198 bp mRNA linear HUM 04-AUG-1995
DEFINITION Human prepromultimerin mRNA, complete cds.
ACCESSION U27109
VERSION U27109.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4198)
AUTHORS Hayward,C.P., Hassell,J.A., Denomme,G.A., Rachubinski,R.A.,
Brown,C. and Kelton,J.G.
TITLE The cDNA sequence of human endothelial cell multimerin. A unique
protein with RGDS, coiled-coil, and epidermal growth factor-like
domains and a carboxyl terminus similar to the globular domain of
complement C1q and collagens type VIII and X
JOURNAL J. Biol. Chem. 270 (31), 18246-18251 (1995)
PUBMED 7629143
REFERENCE 2 (bases 1 to 4198)
AUTHORS Hayward,C.P.
TITLE Direct Submission
JOURNAL Submitted (15-MAY-1995) Catherine P. M. Hayward, Pathology,
McMaster University, HSC 2N32, 1200 Main St. W., Hamilton, Ontario
L8N 3Z5, Canada
FEATURES Location/Qualifiers
source 1..4198
/db_xref="H-InvDB:HIT000218849"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="overlapping clones: mmlambda 4, 5, 7, 11 & 17"
/cell_type="endothelial"
/clone_lib="Clonetech library and libraries VII-91-4 and
VII-91-5 (J Biol Chem 262 page 3718)"
5'UTR 1..71
CDS 72..3758
/codon_start=1
/product="prepromultimerin"
/protein_id="AAC52065.1"
/translation="MKGARLFVLLSSLWSGGIGLNNSKHSWTIPEDGNSQKTMPSASV
PPNKIQSLQILPTTRVMSAEIATTPEARTSEDSLLKSTLPPSETSAPAEGVRNQTLTS
TEKAEGVVKLQNLTLPTNASIKFNPGAESVVLSNSTLKFLQSFARKSNEQATSLNTVG
GTGGIGGVGGTGGVGNRAPRETYLSRGDSSSSQRTDYQKSNFETTRGKNWCAYVHTRL
SPTVTLDNQVTYVPGGKGPCGWTGGSCPQRSQKISNPVYRMQHKIVTSLDWRCCPGYS
GPKCQLRAQEQQSLIHTNQAESHTAVGRGVAEQQQQQGCGDPEVMQKMTDQVNYQAMK
LTLLQKKIDNISLTVNDVRNTYSSLEGKVSEDKSREFQSLLKGLKSKSINVLIRDIVR
EQFKIFQNDMQETVAQLFKTVSSLSEDLESTRQIIQKVNESVVSIAAQQKFVLVQENR
PTLTDIVELRNHIVNVRQEMTLTCEKPIKELEVKQTHLEGALEQEHSRSILYYESLNK
TLSKLKEVHEQLLSTEQVSDQKNAPAAESVSNNVTEYMSTLHENIKKQSLMMLQMFED
LHIQESKINNLTVSLEMEKESLRGECEDMLSKCRNDFKFQLKDTEENLHVLNQTLAEV
LFPMDNKMDKMSEQLNDLTYDMEILQPLLEQGASLRQTMTYEQPKEAIVIRKKIENLT
SAVNSLNFIIKELTKRHNLLRNEVQGRDDALERRINEYALEMEDGLNKTMTIINNAID
FIQDNYALKETLSTIKDNSEIHHKCTSDMETILTFIPQFHRLNDSIQTLVNDNQRYNF
VLQVAKTLAGIPRDEKLNQSNFQKMYQMFNETTSQVRKYQQNMSHLEEKLLLTTKISK
NFETRLQDIESKVTQTLIPYYISVKKGSVVTNERDQALQLQVLNSRFKALEAKSIHLS
INFFSLNKTLHEVLTMCHNASTSVSELNATIPKWIKHSLPDIQLLQKGLTEFVEPIIQ
IKTQAALSNSTCCIDRSLPGSLANVVKSQKQVKSLPKKINALKKPTVNLTTVLIGRTQ
RNTDNIIYPEEYSSCSRHPCQNGGTCINGRTSFTCACRHPFTGDNCTIKLVEENALAP
DFSKGSYRYAPMVAFFASHTYGMTIPGPILFNNLDVNYGASYTPRTGKFRIPYLGVYV
FKYTIESFSAHISGFLVVDGIDKLAFESENINSEIHCDRVLTGDALLELNYGQEVWLR
LAKGTIPAKFPPVTTFSGYLLYRT"
sig_peptide 72..128
mat_peptide 129..3755
/product="multimerin"
misc_structure 627..638
/note="encodes RGDS domain"
misc_structure 876..911
/note="encodes partial EGF-like domain"
misc_structure 1020..1196
/note="encodes putative coiled-coil structures in protein"
misc_feature 1173..1199
/note="encodes sequence confirmed from protein sequencing"
misc_structure 1269..1406
/note="encodes putative coiled-coil structures in protein"
misc_structure 2073..2285
/note="encodes putative coiled-coil structures in protein"
misc_structure 2523..2690
/note="encodes putative coiled-coil structures in protein"
misc_feature 3183..3185
/note="encodes putative tyrosine sulfation site"
misc_feature 3243..3245
/note="encodes putative asparagine hydroxylation site"
misc_structure 3264..3299
/note="encodes EGF-like domain"
misc_structure 3420..3755
/note="encodes putative globular head domain in protein"
3'UTR 3759..4198
regulatory 4179..4184
/regulatory_class="polyA_signal_sequence"
BASE COUNT 1436 a 791 c 808 g 1163 t
ORIGIN
1 ctgctatcaa aaaggccata aggattttgt ccccaaattt cacatgagct accttgcttc
61 aaactactga gatgaagggg gcaagattat ttgtccttct ttctagttta tggagtgggg
121 gcattgggct taacaacagt aagcattctt ggactatacc tgaggatggg aactctcaga
181 agactatgcc ttctgcttca gttcctccaa ataaaataca aagtttgcaa atactgccaa
241 ccactcgggt catgtcggcg gagatagcta caactccaga ggcaagaact tctgaagaca
301 gtcttcttaa atcaacactg cctccctcag aaacaagtgc acctgctgag ggtgtgagaa
361 atcaaactct cacatccaca gagaaagcag aaggagtggt caagttacag aatcttaccc
421 tcccaaccaa cgctagcatc aagttcaatc ctggagcaga atcagtggtc ctttccaatt
481 ctacactgaa atttcttcag agctttgcca gaaagtcaaa tgaacaagca acttctctaa
541 acacagttgg aggcactgga ggcattggag gcgttggagg cactggaggc gtgggaaatc
601 gagccccacg ggaaacatac ctcagccggg gtgacagcag ttccagccaa agaactgact
661 accaaaaatc aaatttcgaa acaactagag gaaagaattg gtgtgcttat gtacatacca
721 ggttatctcc cacagtgaca ttggacaacc aggtcactta tgtcccaggt gggaaaggac
781 cttgtggctg gaccggtgga tcctgtcctc agagatctca gaagatatcc aatcctgtct
841 ataggatgca acataaaatt gtcacctcat tggattggag gtgctgtcct ggatacagtg
901 ggccgaaatg tcaactaaga gcccaggaac agcaaagttt gatacacacc aaccaggctg
961 aaagtcatac agctgttggc agaggagtag ctgagcagca gcagcagcaa ggctgtggtg
1021 acccagaagt gatgcaaaaa atgactgatc aggtgaacta ccaggcaatg aaactgactc
1081 ttctgcagaa gaagattgac aatatttctt tgactgtgaa tgatgtaagg aacacttact
1141 cctccctaga aggaaaagtc agcgaagata aaagcagaga atttcaatct cttctaaaag
1201 gtctaaaatc caaaagcatt aatgtactga taagagacat agtaagagaa caatttaaaa
1261 tttttcaaaa tgacatgcaa gagactgtag cacagctctt caagactgta tcaagtctat
1321 cagaggacct cgaaagcacc aggcaaataa ttcaaaaagt taatgaatct gtggtttcaa
1381 tagcagccca gcaaaagttt gttttggtgc aagagaatcg gcccactttg actgatatag
1441 tggaactaag gaatcacatt gtgaatgtaa ggcaagaaat gactcttaca tgtgagaagc
1501 ctattaaaga actagaagta aagcagactc atttagaagg tgctctagaa caggaacact
1561 caagaagcat tctgtattat gaatccctca ataaaactct ttctaaattg aaggaagtac
1621 atgagcagct tttatcaact gaacaggtat cagaccagaa gaatgctcca gctgctgagt
1681 cagttagcaa taatgtcact gagtacatgt ctactttaca tgaaaatata aagaagcaga
1741 gtttgatgat gctgcaaatg tttgaagatt tgcacattca agaaagcaag attaacaatc
1801 tcaccgtctc tttggagatg gagaaagagt ctctcagagg tgaatgtgaa gacatgttat
1861 ccaaatgcag aaatgatttt aaatttcaac ttaaggacac agaagagaat ttacatgtgt
1921 taaatcaaac attggctgaa gttctctttc caatggacaa taagatggac aaaatgagtg
1981 agcaactaaa tgatttgact tatgatatgg agatccttca acccttgctt gagcagggag
2041 catcactcag acagacaatg acatatgaac aaccaaagga agcaatagtg ataaggaaaa
2101 agatagaaaa tctgactagt gctgtcaata gtctaaattt tattatcaaa gaacttacaa
2161 aaagacacaa cttacttaga aatgaagtac agggtcgtga tgatgcctta gaaagacgta
2221 tcaatgaata tgccttagaa atggaagatg gcctcaataa gacaatgact attataaata
2281 atgctattga tttcattcaa gataactatg ccctaaaaga gactttaagt actattaagg
2341 ataatagtga gatccatcat aaatgtacct ccgatatgga aactattttg acatttattc
2401 ctcagttcca ccgtctgaat gattctattc agactttggt caatgacaat cagagatata
2461 actttgtttt gcaagtcgcc aagacccttg caggtattcc cagagatgag aaactaaatc
2521 agtccaactt ccaaaagatg tatcaaatgt tcaatgaaac cacttcccaa gtgagaaaat
2581 accagcaaaa tatgagtcat ttggaagaaa aactactctt aactaccaag atttccaaaa
2641 attttgagac tcggttgcaa gacattgagt ctaaagttac ccagacgctc ataccttatt
2701 atatttcagt taaaaaaggc agtgtagtta caaatgagag agatcaggct cttcaactgc
2761 aagtattaaa ttccagattt aaggcgttgg aagcaaaatc tatccatctt tcaattaact
2821 tcttttcgct taacaaaact ctccacgaag ttttaacaat gtgtcacaat gcttctacaa
2881 gtgtgtcaga actgaatgct accatcccta agtggataaa acattccctg ccagatattc
2941 aacttcttca gaaaggtcta acagaatttg tggaaccaat aattcaaata aaaactcaag
3001 ctgccctatc taattcaact tgttgtatag atcgatcgtt gcctggtagt ctggcaaatg
3061 ttgtcaagtc tcagaagcaa gtaaaatcat tgccaaagaa aattaacgca cttaagaaac
3121 caacggtaaa tcttaccaca gtcctgatag gccggactca aagaaacacg gacaacataa
3181 tatatcctga ggagtattca agctgtagtc ggcatccgtg ccaaaatggg ggcacgtgca
3241 taaatggaag aactagcttt acctgtgcct gcagacatcc ttttactggt gacaactgca
3301 ctatcaagct tgtggaagaa aatgctttag ctccagattt ttccaaagga tcttacagat
3361 atgcacccat ggtggcattt tttgcatctc atacgtatgg aatgactata cctggtccta
3421 tcctgtttaa taacttggat gtcaattatg gagcttcata taccccaaga actggaaaat
3481 ttagaattcc gtatcttgga gtatatgttt tcaagtacac catcgagtca tttagtgctc
3541 atatttctgg atttttagtg gttgatggaa tagacaagct tgcatttgag tctgaaaata
3601 ttaacagtga aatacactgt gatagggttt taactgggga tgccttatta gaattaaatt
3661 atgggcagga agtctggtta cgacttgcaa aaggaacaat tccagccaag tttccccctg
3721 ttactacatt tagtggctat ttattatatc gtacataagt tagtatgaaa aacagactat
3781 cacctttatt gagaaacagc cagtgttttc atttatcttt gcttgcacat ctgctctgtt
3841 ttggtttttc tacaggaaat gaaaatcaac ttgttttttt aatatgagta aacttgtatg
3901 tctattttat aaaattattt gaatattgtt taatgtctga atatgaaaga gttcttgatc
3961 ctaaagaaat ttagtggcac agaaaacaaa gtgaatttgt tagcataatt attcctattc
4021 ttatttcttc attttaagtc attgcaatgg aaagtaatat tataaaacgg taattacaac
4081 atattatcag tcacagtttt ctttccaatt aaacacttaa cttttgttat tccctgtata
4141 taaatatata acacacattt tctagattca caaatttaaa taaattactc aaaaaatg
//