LOCUS HSU13697 1185 bp mRNA linear HUM 18-MAR-1995
DEFINITION Human interleukin 1-beta converting enzyme isoform beta (IL1BCE)
mRNA, complete cds.
ACCESSION U13697
VERSION U13697.1
KEYWORDS cysteine protease; interleukin 1-beta converting enzyme; apoptotic
protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1185)
AUTHORS Alnemri,E.S., Fernandes-Alnemri,T. and Litwack,G.
TITLE Cloning and expression of four novel isoforms of human
interleukin-1 beta converting enzyme with different apoptotic
activities
JOURNAL J. Biol. Chem. 270 (9), 4312-4317 (1995)
PUBMED 7876192
REFERENCE 2 (bases 1 to 1185)
AUTHORS Alnemri,E.S.
TITLE Direct Submission
JOURNAL Submitted (16-AUG-1994) Emad S. Alnemri, Pharmacology, Jefferson
Cancer Institute, Thomas Jefferson University, 233 S. Tenth Street,
Philadelphia, PA 19107, USA
FEATURES Location/Qualifiers
source 1..1185
/db_xref="H-InvDB:HIT000218144"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="11"
/map="11q23"
/clone="ICE-beta"
/cell_line="Jurkat"
/cell_type="T-lymphocyte"
gene 1..1185
/gene="IL1BCE"
CDS 1..1152
/gene="IL1BCE"
/function="Cleaves pro-interleukin 1-beta to generate
active interleukin 1-beta cytokine"
/note="This is an alternatively spliced isoform which
lacks the entire exon 3 which codes for the C-terminal
part of the propeptide. See D.P. Cerretti et al., Genomics
20:468-473(1994); The gene product of this sequence
designated as ICE-beta is an active protein as determined
by its ability to cause apoptosis; The gene product of the
related sequence, GenBank Accession Number M87507, was
designated as ICE-alpha"
/codon_start=1
/product="Interleukin 1-beta converting enzyme isoform
beta"
/protein_id="AAC50107.1"
/translation="MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVK
RENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSAAPQAVQDNPAM
PTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTG
AEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGI
REGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKD
SVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIE
HMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH"
mat_peptide 295..828
/gene="IL1BCE"
/product="P20 subunit of interleukin 1-beta converting
enzyme isoform beta"
/standard_name="P20 subunit"
/experiment="experimental evidence, no additional details
recorded"
/note="Active interleukin 1-beta converting enzyme is a
heterotetramer made of two P20 and two P10 subunits. The
P20 and P10 subunits are derived from one parental peptide
by proteolytic cleavage; N.A. Thornberry et al., Nature
356:768-774(1992)"
mat_peptide 886..1149
/gene="IL1BCE"
/product="P10 subunit of interleukin 1-beta converting
enzyme isoform beta"
/standard_name="P10 subunit"
/experiment="experimental evidence, no additional details
recorded"
/note="Active interleukin 1-beta converting enzyme is a
heterotetramer made of two P20 and two P10 subunits. The
P20 and P10 subunits are derived from one parental peptide
by proteolytic cleavage; N.A. Thornberry et al., Nature
356:768-774(1992)"
BASE COUNT 362 a 247 c 289 g 287 t
ORIGIN
1 atggccgaca aggtcctgaa ggagaagaga aagctgttta tccgttccat gggtgaaggt
61 acaataaatg gcttactgga tgaattatta cagacaaggg tgctgaacaa ggaagagatg
121 gagaaagtaa aacgtgaaaa tgctacagtt atggataaga cccgagcttt gattgactcc
181 gttattccga aaggggcaca ggcatgccaa atttgcatca catacatttg tgaagaagac
241 agttacctgg cagggacgct gggactctca gcagctcctc aggcagtgca ggacaaccca
301 gctatgccca catcctcagg ctcagaaggg aatgtcaagc tttgctccct agaagaagct
361 caaaggatat ggaaacaaaa gtcggcagag atttatccaa taatggacaa gtcaagccgc
421 acacgtcttg ctctcattat ctgcaatgaa gaatttgaca gtattcctag aagaactgga
481 gctgaggttg acatcacagg catgacaatg ctgctacaaa atctggggta cagcgtagat
541 gtgaaaaaaa atctcactgc ttcggacatg actacagagc tggaggcatt tgcacaccgc
601 ccagagcaca agacctctga cagcacgttc ctggtgttca tgtctcatgg tattcgggaa
661 ggcatttgtg ggaagaaaca ctctgagcaa gtcccagata tactacaact caatgcaatc
721 tttaacatgt tgaataccaa gaactgccca agtttgaagg acaaaccgaa ggtgatcatc
781 atccaggcct gccgtggtga cagccctggt gtggtgtggt ttaaagattc agtaggagtt
841 tctggaaacc tatctttacc aactacagaa gagtttgagg atgatgctat taagaaagcc
901 cacatagaga aggattttat cgctttctgc tcttccacac cagataatgt ttcttggaga
961 catcccacaa tgggctctgt ttttattgga agactcattg aacatatgca agaatatgcc
1021 tgttcctgtg atgtggagga aattttccgc aaggttcgat tttcatttga gcagccagat
1081 ggtagagcgc agatgcccac cactgaaaga gtgactttga caagatgttt ctacctcttc
1141 ccaggacatt aaaataagga aactgtatga atgtctgcgg gcagg
//