LOCUS HSU12387 1774 bp mRNA linear HUM 10-MAY-1995
DEFINITION Human thiopurine methyltransferase (TPMT) mRNA, complete cds.
ACCESSION U12387
VERSION U12387.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1774)
AUTHORS Lee,D., Szumlanski,C., Houtman,J., Honchel,R., Rojas,K.,
Overhauser,J., Wieben,E.D. and Weinshilboum,R.M.
TITLE Thiopurine methyltransferase pharmacogenetics. Cloning of human
liver cDNA and a processed pseudogene on human chromosome 18q21.1
JOURNAL Drug Metab. Dispos. 23 (3), 398-405 (1995)
PUBMED 7628307
REFERENCE 2 (bases 1 to 1774)
AUTHORS Szumlanski,C.L.
TITLE Direct Submission
JOURNAL Submitted (18-JUL-1994) Carol L. Szumlanski, Dept. of Pharmacology,
Mayo Medical School/Mayo Clinic/Mayo Foundation, 200 First St. SW,
Rochester, MN 55905, USA
FEATURES Location/Qualifiers
source 1..1774
/db_xref="H-InvDB:HIT000218080"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="liver"
/clone_lib="Human liver 5' stretch plus cDNA library in
the lambda GT11 vector, Clontech catalog No.HL3006B"
gene 1..1774
/gene="TPMT"
CDS 77..814
/gene="TPMT"
/EC_number="2.1.1.67"
/function="thiopurine methylation; S-methylation"
/note="35 kDa monomer; cytosolic protein"
/codon_start=1
/product="thiopurine methyltransferase"
/protein_id="AAC50130.1"
/translation="MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQ
GHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTE
QNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINP
GDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRCLE
KVDAFEERHKSWGIDCLFEKLYLLTEK"
regulatory 1747..1752
/regulatory_class="polyA_signal_sequence"
/gene="TPMT"
polyA_site 1774
/gene="TPMT"
/note="11 A residues"
BASE COUNT 568 a 306 c 353 g 547 t
ORIGIN
1 gaggcaatgg ccggcaacca gctgtaagcg aggcacggaa gacatatgct tgtgagacaa
61 aggtgtctct gaaactatgg atggtacaag aacttcactt gacattgaag agtactcgga
121 tactgaggta cagaaaaacc aagtactaac tctggaagaa tggcaagaca agtgggtgaa
181 cggcaagact gcttttcatc aggaacaagg acatcagcta ttaaagaagc atttagatac
241 tttccttaaa ggcaagagtg gactgagggt attttttcct ctttgcggaa aagcggttga
301 gatgaaatgg tttgcagacc ggggacacag tgtagttggt gtggaaatca gtgaacttgg
361 gatacaagaa ttttttacag agcagaatct ttcttactca gaagaaccaa tcaccgaaat
421 tcctggaacc aaagtattta agagttcttc ggggaacatt tcattgtact gttgcagtat
481 ttttgatctt cccaggacaa atattggcaa atttgacatg atttgggata gaggagcatt
541 agttgccatt aatccaggtg atcgcaaatg ctatgcagat acaatgtttt ccctcctggg
601 aaagaagttt cagtatctcc tgtgtgttct ttcttatgat ccaactaaac atccaggtcc
661 accattttat gttccacatg ctgaaattga aaggttgttt ggtaaaatat gcaatatacg
721 ttgtcttgag aaggttgatg cttttgaaga acgacataaa agttggggaa ttgactgtct
781 ttttgaaaag ttatatctac ttacagaaaa gtaaatgaga catagataaa ataaaatcac
841 actgacatgt ttttgaggaa ttgaaaatta tgctaaagcc tgaaaatgta atggatgaat
901 ttttaaaatt gtttataaat catatgatag atctttacta aaaatggctt tttagtaaag
961 ccatttactt tttctaaaaa agttttagaa gaaaaagatg taactaaact tttaaagtag
1021 ctcctttgga gaggagatta tgatgtgaaa gattatgcct atgtgtcttg cagattgcaa
1081 gatattttac caatcagcat gtgttacctg tacaattaaa aaaatatttc aaaatgcaat
1141 gcatattaaa tataatacac acagaaaaac tggcatttat tttgttttat ttttttgaga
1201 tggagtttcg ttcttgttgc ccaacctgga gtgcaatggt gcaatctcag ctcactgcaa
1261 cctctgcctc ccaggttcag gtgattctcc tgcctcagcc tcctgagtag ctgggattac
1321 aggtgtgcgc caccacgccc agctaatttt ttgtattttt agtagagaca gggtttcacc
1381 atgttggtca ggctgatctc gagctcctga cctcaggtga tctacccacc tcggcctccc
1441 aaagtgctgg gattacaggc gtgagccact gcacctggcc tgacattctt tatgaaattt
1501 agaattgttg aagaactata acatttcagt agggttcaag gtggtcccaa aagttatata
1561 aaagattagt ttttactata aacccttgtc ttttactcag atcctagcat cccttttcac
1621 atggtttctc catgtatata acagaatcaa gaaacaaatt ttaattaaac aatctgtaac
1681 agaatcaaga aacaaataca ttttaattaa acaatctata tggaacaaac attcccaaat
1741 tctaagaata aatttttctt taagttttct ctga
//