LOCUS HSU09178 3951 bp mRNA linear HUM 28-DEC-1994
DEFINITION Human dihydropyrimidine dehydrogenase mRNA, complete cds.
ACCESSION U09178
VERSION U09178.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3951)
AUTHORS Yokota,H., Fernandez-Salguero,P., Furuya,H., Lin,K., McBride,O.W.,
Podschun,B., Schnackerz,K.D. and Gonzalez,F.J.
TITLE cDNA cloning and chromosome mapping of human dihydropyrimidine
dehydrogenase, an enzyme associated with 5-fluorouracil toxicity
and congenital thymine uraciluria
JOURNAL J. Biol. Chem. 269 (37), 23192-23196 (1994)
PUBMED 8083224
REFERENCE 2 (sites)
AUTHORS Eggink,G., Engel,H., Vriend,G., Terpstra,P. and Witholt,B.
TITLE Rubredoxin reductase of Pseudomonas oleovorans. Structural
relationship to other flavoprotein oxidoreductases based on one NAD
and two FAD fingerprints
JOURNAL J. Mol. Biol. 212 (1), 135-142 (1990)
PUBMED 2319593
REFERENCE 3 (sites)
AUTHORS Porter,D.J., Chestnut,W.G., Merrill,B.M. and Spector,T.
TITLE Mechanism-based inactivation of dihydropyrimidine dehydrogenase by
5-ethynyluracil
JOURNAL J. Biol. Chem. 267 (8), 5236-5242 (1992)
PUBMED 1544906
REFERENCE 4 (sites)
AUTHORS Dupuis,A., Skehel,J.M. and Walker,J.E.
TITLE A homologue of a nuclear-coded iron-sulfur protein subunit of
bovine mitochondrial complex I is encoded in chloroplast genomes
JOURNAL Biochemistry 30 (11), 2954-2960 (1991)
PUBMED 1901022
REFERENCE 5 (sites)
AUTHORS Wierenga,R.K., De Maeyer,M.C.H. and Hol,W.G.J.
TITLE Interaction of pyrophosphatase moieties with alfa-helixes in
dinucleotide binding proteins
JOURNAL Biochemistry 24, 1346-1357 (1985)
REFERENCE 6 (bases 1 to 3951)
AUTHORS Gonzalez,F.J.
TITLE Direct Submission
JOURNAL Submitted (22-APR-1994) Frank J. Gonzalez, National Cancer
Institute, National Institutes of Health, 9000 Rockville Pike,
Bethesda, MD 20892, USA
FEATURES Location/Qualifiers
source 1..3951
/db_xref="H-InvDB:HIT000217887"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="liver"
CDS 82..3159
/codon_start=1
/product="dihydropyrimidine dehydrogenase"
/protein_id="AAA57474.1"
/translation="MAPVLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRN
PDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFIT
SIANKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGPINIGGLQQFAT
EVFKAMSIPQIRNPSLPPPEKMSEAYSAKIALFGAGPASISCASFLARLGYSDITIFE
KQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSVNEMTLSTLKEKGY
KAAFIGIGLPEPNKDAIFQGLTQDQGFYTSKDFLPLVAKGSKAGMCACHSPLPSIRGV
VIVLGAGDTAFDCATSALRCGARRVFIVFRKGFVNIRAVPEEMELAKEEKCEFLPFLS
PRKVIVKGGRIVAMQFVRTEQDETGKWNEDEDQMVHLKADVVISAFGSVLSDPKVKEA
LSPIKFNRWGLPEVDPETMQTSEAWVFAGGDVVGLANTTVESVNDGKQASWYIHKYVQ
SQYGASVSAKPELPLFYTPIDLVDISVEMAGLKFINPFGLASATPATSTSMIRRAFEA
GWGFALTKTFSLDKDIVTNVSPRIIRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQS
VTELKADFPDNIVIASIMCSYNKNDWTELAKKSEDSGADALELNLSCPHGMGERGMGL
ACGQDPELVRNICRWVRQAVQIPFFAKLTPNVTDIVSIARAAKEGGANGVTATNTVSG
LMGLKSDGTPWPAVGIAKRTTYGGVSGTAIRPIALRAVTSIARALPGFPILATGGIDS
AESGLQFLHSGASVLQVCSAIQNQDFTVIEDYCTGLKALLYLKSIEELQDWDGQSPAT
VSHQKGKPVPRIAELMDKKLPSFGPYLEQRKKIIAENKIRLKEQNVAFSPLKRSCFIP
KRPIPTIKDVIGKALQYLGTFGELSNVEQVVAMIDEEMCINCGKCYMTCNDSGYQAIQ
FDPETHLPTITDTCTGCTLCLSVCPIVDCIKMVSRTTPYEPKRGVPLSVNPVC"
misc_feature 1084..1134
/citation=[5]
/function="catalytic cofactor NADPH/NADP binding site"
misc_feature 1492..1524
/citation=[2]
/function="electron transfer center, FAD binding site"
misc_feature 2062..2175
/citation=[3]
/function="uracil (substrate) binding site"
misc_feature 2938..2973
/standard_name="iron-sulfur center"
/citation=[4]
/function="catalytic cofactor [4Fe-4S] binding site"
BASE COUNT 1153 a 785 c 896 g 1117 t
ORIGIN
1 gctgtcactt ggctctctgg ctggagcttg aggacgcaag gagggtttgt cactggcaga
61 ctcgagactg taggcactgc catggcccct gtgctcagta aggactcggc ggacatcgag
121 agtatcctgg ctttaaatcc tcgaacacaa actcatgcaa ctctgtgttc cacttcggcc
181 aagaaattag acaagaaaca ttggaaaaga aatcctgata agaactgctt taattgtgag
241 aagctggaga ataattttga tgacatcaag cacacgactc ttggtgagcg aggagctctc
301 cgagaagcaa tgagatgcct gaaatgtgca gatgccccgt gtcagaagag ctgtccaact
361 aatcttgata ttaaatcatt catcacaagt attgcaaaca agaactatta tggagctgct
421 aagatgatat tttctgacaa cccacttggt ctgacttgtg gaatggtatg tccaacctct
481 gatctatgtg taggtggatg caatttatat gccactgaag agggacccat taatattggt
541 ggattgcagc aatttgctac tgaggtattc aaagcaatga gtatcccaca gatcagaaat
601 ccttcgctgc ctcccccaga aaaaatgtct gaagcctatt ctgcaaagat tgctcttttt
661 ggtgctgggc ctgcaagtat aagttgtgct tcctttttgg ctcgattggg gtactctgac
721 atcactatat ttgaaaaaca agaatatgtt ggtggtttaa gtacttctga aattcctcag
781 ttccggctgc cgtatgatgt agtgaatttt gagattgagc taatgaagga ccttggtgta
841 aagataattt gcggtaaaag cctttcagtg aatgaaatga ctcttagcac tttgaaagaa
901 aaaggctaca aagctgcttt cattggaata ggtttgccag aacccaataa agatgccatc
961 ttccaaggcc tgacgcagga ccaggggttt tatacatcca aagacttttt gccacttgta
1021 gccaaaggca gtaaagcagg aatgtgcgcc tgtcactctc cattgccatc gatacgggga
1081 gtcgtgattg tacttggagc tggagacact gccttcgact gtgcaacatc tgctctacgt
1141 tgtggagctc gccgagtgtt catcgtcttc agaaaaggct ttgttaatat aagagctgtc
1201 cctgaggaga tggagcttgc taaggaagaa aagtgtgaat ttctgccatt cctgtcccca
1261 cggaaggtta tagtaaaagg tgggagaatt gttgctatgc agtttgttcg gacagagcaa
1321 gatgaaactg gaaaatggaa tgaagatgaa gatcagatgg tccatctgaa agccgatgtg
1381 gtcatcagtg cctttggttc agttctgagt gatcctaaag taaaagaagc cttgagccct
1441 ataaaattta acagatgggg tctcccagaa gtagatccag aaactatgca aactagtgaa
1501 gcatgggtat ttgcaggtgg tgatgtcgtt ggtttggcta acactacagt ggaatcggtg
1561 aatgatggaa agcaagcttc ttggtacatt cacaaatacg tacagtcaca atatggagct
1621 tccgtttctg ccaagcctga actacccctc ttttacactc ctattgatct ggtggacatt
1681 agtgtagaaa tggccggatt gaagtttata aatccttttg gtcttgctag cgcaactcca
1741 gccaccagca catcaatgat tcgaagagct tttgaagctg gatggggttt tgccctcacc
1801 aaaactttct ctcttgataa ggacattgtg acaaatgttt cccccagaat catccgggga
1861 accacctctg gccccatgta tggccctgga caaagctcct ttctgaatat tgagctcatc
1921 agtgagaaaa cggctgcata ttggtgtcaa agtgtcactg aactaaaggc tgacttccca
1981 gacaacattg tgattgctag cattatgtgc agttacaata aaaatgactg gacggaactt
2041 gccaagaagt ctgaggattc tggagcagat gccctggagt taaatttatc atgtccacat
2101 ggcatgggag aaagaggaat gggcctggcc tgtgggcagg atccagagct ggtgcggaac
2161 atctgccgct gggttaggca agctgttcag attccttttt ttgccaagct gaccccaaat
2221 gtcactgata ttgtgagcat cgcaagagct gcaaaggaag gtggtgccaa tggcgttaca
2281 gccaccaaca ctgtctcagg tctgatggga ttaaaatctg atggcacacc ttggccagca
2341 gtggggattg caaagcgaac tacatatgga ggagtgtctg ggacagcaat cagacctatt
2401 gctttgagag ctgtgacctc cattgctcgt gctctgcctg gatttcccat tttggctact
2461 ggtggaattg actctgctga aagtggtctt cagtttctcc atagtggtgc ttccgtcctc
2521 caggtatgca gtgccattca gaatcaggat ttcactgtga tcgaagacta ctgcactggc
2581 ctcaaagccc tgctttatct gaaaagcatt gaagaactac aagactggga tggacagagt
2641 ccagctactg tgagtcacca gaaagggaaa ccagttccac gtatagctga actcatggac
2701 aagaaactgc caagttttgg accttatctg gaacagcgca agaaaatcat agcagaaaac
2761 aagattagac tgaaagaaca aaatgtagct ttttcaccac ttaagagaag ctgttttatc
2821 cccaaaaggc ctattcctac catcaaggat gtaataggaa aagcactgca gtaccttgga
2881 acatttggtg aattgagcaa cgtagagcaa gttgtggcta tgattgatga agaaatgtgt
2941 atcaactgtg gtaaatgcta catgacctgt aatgattctg gctaccaggc tatacagttt
3001 gatccagaaa cccacctgcc caccataacc gacacttgta caggctgtac tctgtgtctc
3061 agtgtttgcc ctattgtcga ctgcatcaaa atggtttcca ggacaacacc ttatgaacca
3121 aagagaggcg tacccttatc tgtgaatccg gtgtgttaag gtgatttgtg aaacagttgc
3181 tgtgaacttt catgtcacct acatatgctg atctcttaaa atcatgatcc ttgtgttcag
3241 ctctttccaa attaaaacaa atatacattt tctaaataaa aatatgtaat ttcaaaatac
3301 atttgtaagt gtaaaaaatg tctcatgtca atgaccattc aattagtggc ataaaataga
3361 ataattcttt tctgaggata gtagttaaat aactgtgtgg cagttaattg gatgttcact
3421 gccagttgtc ttatgtgaaa aattaacttt ttgtgtggca attagtgtga cagtttccaa
3481 attgccctat gctgtgctcc atatttgatt tctaattgta agtgaaatta agcattttga
3541 aacaaagtac tctttaacat acaagaaaat gtatccaagg aaacatttta tcaataaaaa
3601 ttacctttaa ttttaatgct gtttctaaga aaatgtagtt agctccataa agtacaaatg
3661 aagaaagtca aaaattattt gctatggcag gataagaaag cctaaaattg agtttgtgga
3721 ctttattaag taaaatcccc ttcgctgaaa ttgcttattt ttggtgttgg atagaggata
3781 gggagaatat ttactaacta aataccattc actactcatg cgtgagatgg gtgtacaaac
3841 tcatcctctt ttaatggcat ttctctttaa actatgttcc taaccaaatg agatgatagg
3901 atagatcctg gttaccactc ttttactgtg cacatatggg ccccggaatt c
//