LOCUS HSU08379 1591 bp mRNA linear HUM 26-OCT-1995
DEFINITION Human Mi-2 autoantigen 240 kDa protein mRNA, partial cds.
ACCESSION U08379
VERSION U08379.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1591)
AUTHORS Ge,Q., Nilasena,D.S., O'Brien,C.A., Frank,M.B. and Targoff,I.N.
TITLE Molecular analysis of a major antigenic region of the 240-kD
protein of Mi-2 autoantigen
JOURNAL J. Clin. Invest. 96 (4), 1730-1737 (1995)
PUBMED 7560064
REFERENCE 2 (bases 1 to 1591)
AUTHORS Frank,M.B.
TITLE Direct Submission
JOURNAL Submitted (06-APR-1994) M. Bart Frank, Oklahoma Medical Research
Foundation, Arthritis and Immunology Program, 825 NE 13th Street,
Oklahoma City, OK 73104, USA
FEATURES Location/Qualifiers
source 1..1591
/db_xref="H-InvDB:HIT000217840"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="C4"
/cell_type="thymocyte"
/tissue_type="thymus"
/clone_lib="thymocyte and 5' stretch thymocyte libraries
(Clontech)"
mRNA <1..>1591
/experiment="experimental evidence, no additional details
recorded"
/note="Northern blot with this cDNA shows hybridization of
a single band in 7.5 kb region"
CDS <1..>1591
/note="The partial cDNA encodes an antigenic region of a
nuclear protein to which autoantibodies are produced by
15-20% of dermatomyositis patients"
/codon_start=2
/product="Mi-2 autoantigen 240 kDa protein"
/protein_id="AAC50228.1"
/translation="PHFQQKQKQVEQKSSATLLLTWGLEDVEHVFSEEDYHTLTNYKA
FSQFMRPLIAKKNPKIPMSKMMTILGAKWREFSANNPFKGSAAAVAAAAAAAAAAVAE
QVSAAVSSATPIAPSGPPALPPPPAADIQPPPIRRAKTKEGKGPGHKRRSKSPRVPDG
RKKLRGKKMAPLKIKLGLLGGKRKKGGSSDEGPEPEAEESDLDSGSVHSASGRPDGPV
RTKKLKRGRPGRKKKKVLGCPAVAGEEEVDGYETDHQDYCEVCQQGGEIILCDTCPRA
YHLVCLDPELDRAPEGKWSCPHCEKEGVQWEAKEEEEEYEEEGEEEGEKEEEDDHMEY
CRVCKDGGELLCCDACISSYHIHCLNPPLPDIPNGEWLCPRCTCPVLKGRVQKILHWR
WGEPPVAVPAPQQADGNPDVPPPRPLQGRSEREFFVKWVGLSYWHCSWAKELQLEIFH
LVMYRNYQRKNDMDEPPPLDYGSGEDDGKSDKRKVKDPHYAEMEEKYYRFGIKPEWMT
VHRIINHSVDKKGNYHYLVKGR"
BASE COUNT 418 a 417 c 478 g 278 t
ORIGIN
1 accccacttc cagcagaagc aaaagcaagt ggaacagaag tcatcagcaa ctctgcttct
61 gacctggggc ctggaggatg tggagcatgt gttctctgag gaggattacc acacgctcac
121 caactacaaa gccttcagcc agttcatgag gcccctaatt gctaagaaga atcctaagat
181 cccaatgtct aagatgatga ccatccttgg ggccaaatgg agagagttca gtgccaacaa
241 ccccttcaag gggtcagcag ctgctgtggc ggcggcagcg gcagcagcag cagcagctgt
301 agctgagcag gtgtcagctg ctgtctcgtc ggccaccccc atagcaccct ccggaccccc
361 cgcccttcca ccaccccctg ctgctgatat ccagccccca cccatccgaa gagccaaaac
421 caaagagggc aaaggtccag gccataagag gcggagtaag agcccccgag tgcctgatgg
481 acgcaagaag cttcggggaa agaaaatggc accactcaaa ataaaactag ggcttctggg
541 tggcaagagg aagaaaggag gctcgagcga cgaaggtcct gaaccagagg ctgaggaatc
601 agacctggac agtggcagtg tccacagtgc ctcaggccgg cctgatggcc ctgtccgcac
661 caagaaacta aagagaggcc ggccaggaag gaagaagaag aaggtcctgg gctgtcctgc
721 agtggccggg gaggaggagg ttgatggcta cgagacggat caccaggatt actgtgaggt
781 gtgccagcag ggtggggaaa ttattctgtg tgacacctgc cctcgtgcct accacctcgt
841 ctgccttgat cctgagcttg accgggctcc agagggcaaa tggagctgcc ctcactgtga
901 gaaggagggg gtccagtggg aggccaagga ggaagaagaa gaatacgaag aggagggaga
961 ggaagaaggg gagaaggagg aggaggatga tcacatggag tactgccgcg tatgcaagga
1021 cggcggggag ctcctgtgct gtgacgcgtg catctcctcc taccacattc attgtctaaa
1081 ccctcccctg cctgacattc ccaatggtga atggctgtgt ccccgatgca catgccccgt
1141 gctgaagggt cgagtgcaga agatcctaca ttggcggtgg ggggagccac ctgtagcagt
1201 gccagcccct caacaggcag atggaaatcc agatgtccca cccccccgtc ctcttcaagg
1261 cagatcagag cgagagttct ttgtcaagtg ggtaggacta tcctactggc actgctcctg
1321 ggccaaggag cttcagctgg aaatcttcca tttggttatg tatcgaaact accagcggaa
1381 gaatgacatg gatgagcccc cacccctgga ctatggctcc ggcgaggatg atgggaagag
1441 cgacaagcgt aaagtgaaag acccgcacta tgctgagatg gaggagaagt actatcgttt
1501 tggcatcaag ccagagtgga tgaccgtcca ccgcatcatc aaccacagtg tggataaaaa
1561 ggggaattac cactatctag taaaagggag g
//